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Functional nano-crystalline cellulose hydrogel and preparation method thereof

A nanocellulose and oxidized cellulose technology, applied in medical science, prosthesis, etc., can solve problems such as unfavorable cell mixing, harsh conditions, and inability of cells to grow and migrate, and achieve good cell compatibility, high water content, biological good compatibility

Active Publication Date: 2019-01-11
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods either need to introduce biotoxic chemical cross-linking agents, or need to modify the cellulose, or the conditions are relatively harsh (such as heating or freezing), which is not conducive to mixing directly with cells, and usually the prepared hydrogel With a nanopore structure, cells can only grow on the surface of the gel, and cells cannot grow and migrate inside the gel

Method used

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  • Functional nano-crystalline cellulose hydrogel and preparation method thereof
  • Functional nano-crystalline cellulose hydrogel and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0033] A functionalized nanocellulose hydrogel for 3D bioprinting, the preparation method is as follows:

[0034] 1) Add 0.01g TEMPO and 0.1g NaBr into 100mL deionized water, stir magnetically at 10°C and 300r / min for 10min to completely dissolve TEMPO and NaBr, then add 1g of cotton linters (length 2-3mm ) and 1gNaClO, adjust the pH of the system to 8 by adding 0.1mol / L NaOH solution dropwise, after reacting at 10°C for 3h, continue to add 1gNaClO 2 Stir and react at room temperature for 1 day, filter the oxidized cellulose pulp, wash with deionized water for more than 3 times, and dry to obtain TEMPO oxidized cellulose (TOC). 2 Perform acidification to oxidize incompletely oxidized aldehyde groups into carboxylate groups, which is conducive to better dispersion of nanofibers;

[0035] 2) Prepare TOCN dispersion: add 0.1g TOC to 99.9g distilled water and mechanically stir at 1600r / min for 10min to obtain 0.1wt% TOCN dispersion;

[0036]3) Preparation of nanocellulose gel: T...

Embodiment 2

[0039] A functionalized nanocellulose hydrogel for 3D bioprinting, the preparation method is as follows:

[0040] 1) Add 0.1g TEMPO and 0.1g NaBr to 100mL deionized water, stir magnetically at 10°C and 300r / min for 10min to completely dissolve TEMPO and NaBr, then add 1g cotton linters (length 2-3mm) to the above system and 1gNaClO, by dropping 0.1mol / L NaOH solution to adjust the pH value of the system to 8, after reacting at 10°C for 4h, continue to add 1gNaClO 2 Stir and react at room temperature for 1 day, filter the oxidized cellulose pulp, wash with deionized water for more than 3 times, and dry to obtain TEMPO oxidized cellulose (TOC);

[0041] 2) Prepare TOCN dispersion: add 0.1g TOC to 99.9g distilled water and mechanically stir at 1600r / min for 10min to obtain 0.1wt% TOCN dispersion;

[0042] 3) Preparation of nanocellulose gel: Concentrate the above TOCN dispersion to a concentration of 0.2%, heat it in a sterilized pot at 121°C for 30 minutes, and then mix the 0.2...

Embodiment 3

[0045] A 3D bioprinted nanocellulose gel can be prepared by the following method:

[0046] 1) Add 0.01g TEMPO and 0.1g NaBr to 100mL deionized water, stir magnetically at 10°C and 300r / min for 10min to completely dissolve TEMPO and NaBr, then add 1g cotton linters (length 2-3mm) to the above system and 0.5gNaClO, adjust the pH value of the system to 8 by adding 0.1mol / L NaOH solution dropwise, after reacting at 10°C for 3h, continue to add 0.5gNaClO 2 Stir and react at room temperature for 1 day, filter the oxidized cellulose pulp, wash with deionized water for more than 3 times, and dry to obtain TEMPO oxidized cellulose (TOC);

[0047] 2) Prepare TOCN dispersion: add 0.1g TOC to 99.9g distilled water and mechanically stir at 1600r / min for 10min to obtain 0.1wt% TOCN dispersion;

[0048] 3) Preparation of nanocellulose gel: Concentrate the above TOCN dispersion to 0.5%, heat it in a sterilized pot at 121°C for 30 minutes, and then mix the nanocellulose solution with a concen...

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Abstract

The invention relates to functional nano-crystalline cellulose hydrogel and a preparation method thereof. The interior of the hydrogel is of a uniform porous mesh structure, and the pore size of the hydrogel is 30-100 microns. The hydrogel is prepared by the following steps: dispersing TEMPO oxidized cellulose in water to prepare nano-cellulose dispersion, mixing with cells to prepare bio-ink, andprinting into an ionic crosslinking agent by utilizing a 3D bio-printing technology, thereby obtaining the product. The shape of the product can be designed according to needs. The renewable resourcecellulose serves as a raw material, and the functional nano-crystalline cellulose hydrogel with advantages of being excellent in mechanical property, excellent in biocompatibility and applicable to bio-printing is prepared. The prepared hydrogel serves as a nano-cellulose based tissue engineering material, is particularly one of tissues such as bones, cartilage, skin, blood vessels, liver, heartand the like, the application range of the cellulose is widened, and a novel pathway is provided for application research of the cellulose in the biomedical field.

Description

technical field [0001] The invention belongs to the field of biological materials, and relates to a functionalized nano-cellulose hydrogel and a preparation method thereof. Background technique [0002] 3D bioprinting technology is a method of using computer-assisted transfer process to simultaneously write living cells and biomaterials in a predetermined layer-by-layer stacking manner to prepare bioengineered tissues. 3D bioprinting technology uses water dispersion and cells as the main raw materials, and the main form of the product is hydrogel. There are many requirements for hydrogel, such as good biocompatibility, and no clogging of needles, easy to print, and at the same time during the printing process The shear force has little damage to cells, so there are not many hydrogels that can be used for 3D bioprinting. [0003] As a polysaccharide with a crystalline structure, cellulose is the most abundant natural polymer on the earth. The cellulose hydrogel processed fro...

Claims

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Application Information

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IPC IPC(8): C08J9/00C08J3/075C08J3/24C08L1/04A61L27/20A61L27/38A61L27/56A61L27/52A61L27/50
CPCA61L27/20A61L27/3834A61L27/50A61L27/52A61L27/56C08J3/075C08J3/24C08J9/00C08J2301/04C08L1/04
Inventor 石竹群徐海宇杨全岭刘妍熊传溪
Owner WUHAN UNIV OF TECH
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