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Preparation method of paracetamol ratio fluorescence sensor based on copper nanocluster/carbon dot/arginine compound

A technology of copper nano-clusters and paracetamol, which is applied in the direction of nano-medicine, chemical instruments and methods, nano-technology, etc., to achieve the effect of simple operation, strong anti-interference ability and good accuracy

Active Publication Date: 2019-01-08
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no domestic and foreign literatures and patent reports on the use of CuNCs / CDs / arginine complexes to construct ratiometric fluorescent sensors, and the use of ratiometric fluorescence methods to detect paracetamol

Method used

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  • Preparation method of paracetamol ratio fluorescence sensor based on copper nanocluster/carbon dot/arginine compound
  • Preparation method of paracetamol ratio fluorescence sensor based on copper nanocluster/carbon dot/arginine compound

Examples

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Effect test

Embodiment 1

[0023] The preparation method of the paracetamol ratiometric fluorescent sensor based on the copper nanocluster / carbon dot / arginine complex involved in this example, its preparation process and the schematic diagram of the principle of ratiometric fluorescence detection of paracetamol are as follows figure 1 As shown, the specific process steps are as follows:

[0024] Preparation of CuNCs: Dissolve DNA dry powder in ultrapure water to prepare DNA solution, dilute to pH 7.5 with buffer containing 3-morpholine propane sulfonic acid and NaCl, the concentrations of 3-morpholine propane sulfonic acid and NaCl are 10 mM and 15 mM, respectively , add ascorbic acid to the above DNA mixed solution, adjust the final concentration of ascorbic acid to 2mM, add CuCl dropwise at room temperature and under magnetic stirring 2 , CuCl2 The final concentration was adjusted to 1mM, and the mixed solution was reacted in a dark place for 20min. The product solution was dialyzed with a dialysis ba...

Embodiment 2

[0028] Example 2: The preparation process of the paracetamol ratiometric fluorescence sensor involved in this example and the schematic diagram of the principle of the ratiometric fluorescence detection of paracetamol, the process steps for the preparation of CuNCs and CDs are the same as in Example 1, wherein the average size of CuNCs is 3nm, and the average size of CDs is 4nm. Other specific process steps are as follows:

[0029] Preparation of CuNCs / CDs / arginine complex: under magnetic stirring, add CDs aqueous dispersion dropwise to CuNCs aqueous dispersion to form CuNCs / CDs homogeneous mixture, and then add arginine dropwise to this mixture acid aqueous solution to form a homogeneous CuNCs / CDs / arginine complex mixture, in which the concentrations of CuNCs, CDs and arginine were 5 mg mL -1 , 5mgmL -1 and 20 μM.

[0030] At room temperature and under magnetic stirring, a certain amount of paracetamol was added to the CuNCs / CDs / arginine complex mixture to form a homogeneou...

Embodiment 3

[0031] Example 3: The preparation process of the paracetamol ratiometric fluorescence sensor involved in this example and the schematic diagram of the principle of the ratiometric fluorescence detection of paracetamol. The process steps for the preparation of CuNCs and CDs are the same as in Example 1, wherein the average size of CuNCs is 3nm, and the average size of CDs is 5nm. Other specific process steps are as follows:

[0032] Preparation of CuNCs / CDs / arginine complex: under magnetic stirring, add CDs aqueous dispersion dropwise to CuNCs aqueous dispersion to form CuNCs / CDs homogeneous mixture, and then add arginine dropwise to this mixture acid aqueous solution to form a homogeneous CuNCs / CDs / arginine complex mixture, in which the concentrations of CuNCs, CDs and arginine were 2mg mL -1 , 5mgmL -1 and 10 μM.

[0033] At room temperature and under magnetic stirring, a certain amount of paracetamol was added to the CuNCs / CDs / arginine complex mixture to form a homogeneous...

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Abstract

The invention specifically relates to a preparation method of a paracetamol ratio fluorescence sensor based on a copper nanocluster / carbon dot / arginine compound. The preparation method comprises the following steps: combining red fluorescent copper nanoclusters CuNCs with blue fluorescent carbon dots CDs by action of electrostatic adsorption and hydrogen bonds, and adding arginine to form a CuNCs / CDs / arginine compound, wherein blue fluorescence of CDs is weakened by adding arginine and then paracetamol is added, arginine and CDs are separated as the paracetamol is specifically combined with arginine, blue fluorescence of CDs is recovered and arginine and paracetamol do not cause remarkable change of fluorescence of CuNCs; responded to fluorescence of CDs, fitting a linear relationship between the fluorescent peak intensity ratio of CDs / CuNCs and concentration of paracetamol to establish the paracetamol ratio fluorescence sensor. Compared with the prior art, the method is simple to operate, high in interference resistance of ratio signals, good in accuracy and high in sensitivity and selectivity, and can be used as a novel ratio fluorescence sensor to detect paracetamol effectively.

Description

Technical field: [0001] The invention belongs to the technical field of preparation of nanocomposite materials and fluorescent sensors, and in particular relates to a method for preparing a paracetamol ratio fluorescent sensor based on a copper nanocluster / carbon dot / arginine compound, and the prepared sensor can be used for high sensitivity of paracetamol and highly selective quantitative detection. Background technique: [0002] Paracetamol, also known as acetaminophen, is a commonly used phenolic analgesic drug with anti-inflammatory and antipyretic effects. Paracetamol is also a popular over-the-counter analgesic and antipyretic drug. Small doses of paracetamol are beneficial to the human body, but overdose can cause health problems, such as kidney failure and liver necrosis. The overdose of paracetamol is one of the main causes of liver failure. The development of a simple, accurate and quantitative detection method for paracetamol in human serum is of great significan...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432A61K31/167B82Y5/00C09K11/06G01N21/6489G01N2021/7786
Inventor 金辉桂日军卜祥宁付永鑫
Owner QINGDAO UNIV
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