Preparation method of tetracycline carbon dot fluorescent molecular imprinting material

A carbon dot fluorescence and molecular imprinting technology is applied in the field of preparation of carbon dot molecularly imprinted fluorescent composite materials, which can solve the problems of complex and expensive instruments and equipment, and achieve the effects of easy control of reaction conditions, simple experimental operation and good stability.

Inactive Publication Date: 2019-01-08
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in these methods, complex and expensive instruments and equipment or complex sample pretreatment are usually required, and these shortcomings make them have certain limitations in practical application.

Method used

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  • Preparation method of tetracycline carbon dot fluorescent molecular imprinting material
  • Preparation method of tetracycline carbon dot fluorescent molecular imprinting material
  • Preparation method of tetracycline carbon dot fluorescent molecular imprinting material

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The invention provides a method for preparing a tetracycline carbon dot fluorescent molecularly imprinted composite material. The specific preparation route is as follows: figure 1 Shown:

[0034] (1) Dissolve 10g of citric acid (CA) in 40ml of deionized water, add 4.2g of magnesium hydroxide (Mg(OH) 2 ) and 5ml of ethylenediamine (EDA), stirred until the solution was colorless and clear, reacted in a reaction kettle at 200°C for 3 hours, and the resulting product was dialyzed for 48 hours in a dialysis bag and freeze-dried for 24 hours to obtain highly fluorescent carbon dots ( CDs), protected from light, sealed and stored in a refrigerator at 4°C.

[0035] (2) Add 3 mg of carbon dots (CDs) into a solution of 4.5 ml of methanol and 0.5 ml of deionized water, and ultrasonically treat it to dissolve and disperse evenly. Then add 44.443 mg tetracycline (TC), 0.8 mmol methacrylic acid (MAA), ultrasonic treatment, then add 7 mmol ethylene glycol dimethacrylate (EGDMA) and...

Embodiment 2

[0043] Research embodiment 1 obtains the adsorption performance of fluorescent composite material (such as Figure 6 shown). Put 1 mg of fluorescent molecular imprinted composite material in a 2 ml centrifuge tube, add 2 ml of different concentrations (0, 0.05, 5, 20, 40, 60, 80, 100, 140, 180 μg L -1 ) TC standard solution, shake at room temperature for 3.5h, and measure the fluorescence intensity value with a spectrofluorometer (λ=360nm). At the same time, the adsorption experiment of non-imprinted composite materials on TC was done in parallel. from Figure 6 It can be seen that with the increase of TC concentration, the fluorescence intensity weakens, and finally maintains a balance gradually. At the same concentration, the fluorescence quenching of CDs@MIP was greater than that of CDs@NIP.

Embodiment 3

[0045] Research Example 1 obtains the adsorption kinetics performance (such as Figure 7 shown). Weigh 1mg of fluorescent molecularly imprinted and non-imprinted composite materials into 2ml centrifuge tubes, add 2ml of 100μg L -1 TC standard solution, shake at room temperature for 0, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 min respectively, and measure the fluorescence intensity with a fluorescence spectrophotometer. like Figure 7 As shown, the adsorption of the imprinted composite to the target almost reached equilibrium at 210 min.

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Abstract

The invention provides a preparation method of a tetracycline carbon dot fluorescent molecular imprinting material. The method comprises the following steps: adopting CDs as a carrier, adopting methanol / water as a solvent, and adding tetracycline, methacrylic acid, ethylene glycol dimethacrylate and azodiisobutyronitrile to form the tetracycline carbon dot fluorescent molecular imprinting material. The method has the advantages that green and stable carbon dots are taken as fluorescence detection signals, and are combined with the selectivity of a molecular imprinting technology, so that the fluorescent molecular imprinting composite material CDs@MIP with high selectivity on tetracycline is synthesized; the preparation technology is scientific, the cost is low, the method is simple to operate, and reaction conditions are easy to control; and the prepared tetracycline adsorption function material is applicable to adsorption detection on trace tetracycline in water environment, and has an excellent application prospect.

Description

technical field [0001] The invention belongs to the research field of polymer optical sensing material preparation technology and antibiotic detection method, and in particular relates to a preparation method of a carbon dot molecularly imprinted fluorescent composite material and its application in recognition and detection of tetracycline. Background technique [0002] Tetracyclines (TCs) have been widely used in veterinary medicine because of their good oral absorption capacity, low toxicity, low cost and broad-spectrum antibacterial activity. However, there are certain safety hazards in the residual tetracycline: consumption of animal meat and other products may cause allergic reactions in people, in addition, the residue may also increase the resistance of bacteria to antibiotics, and what is worse, water and soil environments are also will be polluted. Due to the potential toxicity to human health, many countries have restricted the use of tetracyclines and made stric...

Claims

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Application Information

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IPC IPC(8): C08F222/14C08F220/06C09K11/06G01N21/64B01J20/281
CPCB01J20/281C08F222/10C09K11/06C08F222/102G01N21/6428G01N2021/6432C08F220/06
Inventor 方国臻赵悦清胡雪莲赵鹏王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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