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Electrochemical biosensor for detecting inorganic trivalent arsenic and detection method thereof

A technology of biosensors and detection methods, applied in electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problem of less use of arsenic ions in detection, and achieve the effects of strong anti-interference ability, high detection sensitivity and simple operation.

Inactive Publication Date: 2019-01-04
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection technology based on nucleic acid aptamer has also been successfully applied to the qualitative analysis and quantitative detection of many substances, but the application of detection of arsenic ions is still relatively small

Method used

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  • Electrochemical biosensor for detecting inorganic trivalent arsenic and detection method thereof
  • Electrochemical biosensor for detecting inorganic trivalent arsenic and detection method thereof
  • Electrochemical biosensor for detecting inorganic trivalent arsenic and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Biosensor and method for detecting inorganic trivalent arsenic using the biosensor such as figure 1 As shown, the details are as follows:

[0039] 1) Preparation of measurement electrodes: the bare gold electrodes were respectively made of Al 2 o 3 Powder polishing for 5 minutes, followed by ultrasonic cleaning with absolute ethanol and ultrapure water for five minutes, soaking in piranha (concentrated H 2 SO 4 with 30wt%H 2 o 2 According to the ratio of 3:1 (V / V) mixed uniformly) in the solution for 10 minutes, washed with ultrapure water for five minutes, and dried under nitrogen flow. Finally with 0.5M H 2 SO 4 The gold electrode was activated by cyclic voltammetry, with the potential set at -0.2-1.6V and the rate of 0.05V / S until a stable cyclic voltammetry was obtained.

[0040] 2) Modification of the measurement electrode: put 10 μL of 100 μM DNA into a PCR machine for annealing at 95°C for 3 minutes, then drop to 25°C suddenly, then add TCEP to open the d...

Embodiment 2

[0047] An electrochemical biosensor for detecting inorganic trivalent arsenic, the biosensor includes a working electrode, a counter electrode and a reference electrode, the working electrode is a measuring electrode, the counter electrode is a platinum electrode, and the reference electrode is an Ag / AgCl electrode. The measuring electrode is a gold electrode, and the surface of the measuring electrode is modified by a nucleic acid aptamer, and the sequence of the nucleic acid aptamer is 5'-SH-ACAGAACAACCAACGTCGCTCCGGGTACTTCTTC-Fc-3'.

[0048] The method for modifying the measuring electrode comprises the following steps:

[0049] 1) Use Al for the measuring electrode 2 o 3 After powder polishing, perform ultrasonic cleaning, and then immerse the measuring electrode in H 2 SO 4 with H 2 o 2 in the mixed solution for 5min, then washed, dried, and pretreated; then cyclic voltammetry was used, and H 2 SO 4 Activate the measuring electrode, set the potential to -0.2-1.6V, a...

Embodiment 3

[0054] An electrochemical biosensor for detecting inorganic trivalent arsenic, the biosensor includes a working electrode, a counter electrode and a reference electrode, the working electrode is a measuring electrode, the counter electrode is a platinum electrode, and the reference electrode is an Ag / AgCl electrode. The measuring electrode is a gold electrode, and the surface of the measuring electrode is modified by a nucleic acid aptamer, and the sequence of the nucleic acid aptamer is 5'-SH-ACAGAACAACCAACGTCGCTCCGGGTACTTCTTC-Fc-3'.

[0055] The method for modifying the measuring electrode comprises the following steps:

[0056] 1) Use Al for the measuring electrode 2 o 3 After powder polishing, perform ultrasonic cleaning, and then immerse the measuring electrode in H 2 SO 4 with H 2 o 2 in the mixed solution for 15min, then washed, dried, and pretreated; then cyclic voltammetry was used, and H 2 SO 4 Activate the measuring electrode, set the potential to -0.2-1.6V, ...

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Abstract

The invention relates to an electrochemical biosensor for detecting inorganic trivalent arsenic and a detection method thereof. The biosensor comprises a determination electrode, wherein the surface of the determination electrode is modified by an aptamer, the sequence of the aptamer is 5'-SH-ACAGAACAACCAACGTCGCTCCGGGTACTTCTTC-Fc-3'; the detection method comprises the following steps that (1) a standard curve is drawn by using an electrochemical analysis method; (2) a to-be-measured solution is measured, and compared with the standard curve, the concentration of the inorganic trivalent arsenicin the to-be-measured solution can be measured. Compared with the prior art, DNA aptamer modification is carried out on the surface of the determination electrode, a detection system of As (III) is constructed by using an electrochemical workstation, based on the change of an electrochemical signal, the quantitative detection of the As (III) is achieved by determining peak current, the operationis simple, the cost is low, the capacity of resisting disturbance is strong, the detection sensitivity is high, the specificity is strong, and the efficient detection of the As (III) can be achieved.

Description

technical field [0001] The invention belongs to the technical field of element detection, and relates to an electrochemical biosensor and a detection method for detecting inorganic trivalent arsenic. Background technique [0002] Nowadays, the process of industrialization is accelerating, and the resulting series of heavy metal pollution is becoming more and more serious, especially in water bodies. About 90% of China's groundwater is polluted to varying degrees, of which 60% are seriously polluted, while inorganic trivalent arsenic is It is a substance that seriously pollutes water resources, and arsenic-contaminated water is often used to irrigate crops, causing soil and crops to be polluted by arsenic, thus posing a serious threat to the environment and human health. Atomic arsenic is basically non-toxic, but its oxide and arsenate are highly toxic, and trivalent arsenic is more toxic than pentavalent arsenic. Arsenic compounds can be absorbed through the respiratory tra...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/416G01N27/48
CPCG01N27/3277G01N27/416G01N27/48
Inventor 袁敏徐斐费佳钰叶泰于劲松曹慧
Owner UNIV OF SHANGHAI FOR SCI & TECH
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