Preservation method of hilsa herring sperm

Abstract

The invention discloses a preservation method of a hilsa herring sperm. The preservation method comprises mixing an antifreeze agent, the hilsa herring sperm and a diluent at the volume ratio of 1:1:5, and preserving at the temperature of 5 DEG C, wherein the diluent comprises the ingredients in percent by volume: 50% of sodium chloride injection, 15-20% of pure water, 7-14% of potassium chloride,5-10% of calcium chloride, 5-10% of sodium bicarbonate, 1-2% of chlorogenic acid, 1-3% of glycerinum, 3-5% of glucose, 8-15% of lecithin, 1-3% of sodium citrate, 1-2% of streptomycin, 1-3% of poly-sodium glutamate, 1-3% of bifidobacterium, and the balance of lactic acid bacteria by volume; and the antifreeze agent comprises the ingredients in percent by volume: 30% of dimethyl sulfoxide, 20% of glycerinum, 30% of ethylene glycol, and 20% of dimethylacetamide. The preservation method effectively inhibits the growth and propagation of harmful microbes, reduces the metabolic rate of the sperm, effectively keeps the preservation quality of the hilsa herring sperm, and saves the production cost, and operation steps are convenient.

Description

technical field [0001] The invention relates to the technical field of aquatic reproduction, in particular to a preservation method for shad semen. Background technique [0002] Shad is a migratory fish that goes upriver to spawn. It is very precious because it enters the river in early summer every year and does not appear at other times. Therefore, people attach great importance to the reproduction of shad. There are mainly two kinds of semen used in artificial insemination of shad. , that is, fresh semen and frozen semen, the former is often referred to as fresh semen, and the latter is referred to as frozen semen. In order to improve the utilization rate of shad species and facilitate the storage and transportation of semen, some shad species farms often produce frozen semen for breeding of shad shad. However, the equipment for producing frozen semen is expensive, liquid nitrogen needs to be replenished in time during storage and transportation, the technical requiremen...

AI Technical Summary

Problems solved by technology

However, the equipment for producing frozen semen is expensive, liquid nitrogen needs to be replenished in time during storage and transportation, the technical requirements for semen thawing are relatively high, and the fertilization rate of shad frozen semen is low. Farms and farmers; therefore, the use of frozen semen greatly limits the popularization and application of shad estrus synchronization and artificial insemination related technologies;

[0003] The production line often uses fresh semen to dilute with normal saline. This method can only meet the production needs of on-site semen collection, on-site dilution, and instant insemination. At present, there are a large number of shad frozen semen diluents and diluted preservation solutions on the market; There are few fresh semen diluents and dilute preservation solutions, and their main components are mostly glucose, sodium citrate, and antibiotics. The sperm motility rate, plasma membrane integrity rate, and acrosome integrity rate are not ideal, and there will be some symptoms after 48 hours. significant reduction