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A kind of human placental mesenchymal stem cell transport liquid

A technology of stem cells and human placenta, applied in the field of stem cells, can solve the problems of reducing the number of living cells and being unsuitable for long-distance transportation, and achieve the effect of improving the survival rate

Active Publication Date: 2021-01-29
深圳华大基因细胞科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of viable cells of the three types of cells (human amniotic fluid, umbilical cord, and placenta-derived mesenchymal stem cells) decreased significantly after being placed in the cell transport solution at room temperature for 72 hours, which is not suitable for long-distance transport

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] 1. Experimental materials

[0015] Choose fresh placentas from healthy puerperas, sign the informed consent form, and agree to be used in scientific research experiments.

[0016] Human placental mesenchymal stem cell culture medium was purchased from Saiye Company; fetal bovine serum was purchased from American Thermo Company; DMEM medium was purchased from American Gibco Company; The culture medium was purchased from Shanghai Raas Blood Products Co., Ltd.; the stem cell osteogenic differentiation medium was purchased from PromoCell; the neural differentiation medium: 20 μg / L epidermal growth factor and 20 μg / L were added to the DMEM / F12 medium containing 2% B27 Formulated with Basic Fibroblast Growth Factor of L.

[0017] 2. Experimental method

[0018] 1. Isolation and culture of human placental mesenchymal stem cells (hPMSCs)

[0019] Peel off the amniotic membrane, take the placental tissue from the fetal face, cut it into pieces, wash it several times with PBS ...

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Abstract

The invention relates to a human placenta mesenchymal stem cell transport solution. The present invention adds 5 μ M heterophylla cyclic peptide C on the basis of the prior art, which can effectively overcome the obvious deficiency of the cell viability reduction after being suspended for 72 hours in the transport liquid of the prior art. The formula of the transport liquid of the present invention is: 30% of 0.2% trehalose solution (PBS solution of trehalose), 25% red blood cell storage solution, 5% 0.2% heparin calcium solution (heparin calcium PBS solution) and 40% human albumin injection are mixed uniformly at room temperature. And contain 5μM heterophyllin C. The transport solution can not only improve the viability of human placental mesenchymal stem cells transported for 72 hours, but also not affect their multidirectional differentiation ability.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a human placenta mesenchymal stem cell transport solution. Background technique [0002] Stem cells are a class of cells with self-renewal and multilineage differentiation capabilities. It is because of its regenerative potential and plasticity that it is widely used in regenerative medicine and stem cell research. Stem cells offer hope for many diseases that currently lack effective treatments, including stroke, Huntington's disease, Alzheimer's disease and Parkinson's disease. [0003] Mesenchymal stem cells are multipotent stromal cells isolated from adult and fetal tissues, defined as adherent fibroblast-like cells. Mesenchymal stem cells have intrinsic homing ability, can migrate to damaged tissues, and actively participate in tissue repair. Although mesenchymal stem cells are pluripotent, their self-renewal ability gradually decreases leading to stagnation of cell division, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 卢珊珊郑桂纯
Owner 深圳华大基因细胞科技有限责任公司
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