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Petroleum hydrocarbon degrading bacterium and fermentation method thereof

A technology of petroleum hydrocarbon degrading bacteria and fermentation method, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low degradation efficiency, slow biochemical degradation speed, and complicated bacterial culture process, and achieve the fermentation cycle. The effect of short, simple production process and easy-to-obtain ingredients

Active Publication Date: 2018-12-28
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The shortcomings of the existing technology are: the biochemical degradation speed of the current oily sewage or sludge is slow, the degradation efficiency is low, and the bacterial cultivation process is complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A genus of Bacillus brevis (named Brevibacillus parabrevis in Latin literature), strain name: GWN-2, preserved in the General Microorganism Collection Center of the China Committee for the Collection of Microorganisms, and the preservation number: CGMCC No.13903.

[0023] Its gene sequence is shown in the sequence listing.

[0024] Use this type of Bacillus brevus to ferment to obtain petroleum hydrocarbon degrading bacteria products. The specific fermentation method is carried out according to the following steps:

[0025] 1) Streak inoculation of Bacillus pseudobrevius preserved in glycerol at -70°C on a solid medium in a pre-sterilized Petri dish in an ultra-clean workbench. The medium is composed of the following components in terms of mass percentage: 0.4% yeast extract, 0.8% peptone, 1.5% agar powder, and water as the balance, pH 7.0, sterilized at 120°C and 0.11Mpa for 30min. The culture dish was placed upside down in an incubator at 33° C. for 3 days to obtain ...

Embodiment 2

[0031] Utilize the class Bacillus brevus of embodiment 1 to carry out fermentation, obtain petroleum hydrocarbon degrading bacterium product, concrete fermentation method is carried out according to following steps:

[0032] 1) Streak-inoculate the Bacillus brevis preserved in glycerol at -70°C on the solid medium in the pre-sterilized petri dish in the ultra-clean workbench (the medium is composed of the following components according to the mass percentage: yeast extract 0.5%, peptone 1%, agar powder 1.8%, the balance is water, pH 7.2, 120℃, 0.11 ± 0.02Mpa continuous sterilization for 30min). The culture dish was placed upside down in an incubator at 33° C. for 3 days to obtain a single colony of activated Bacillus pseudobrevius.

[0033]2) Pick a single colony and suspend it in 20ml sterilized distilled water aseptically. Sterilize the pipette to draw 1ml and inoculate it on the slant of solid medium in a sterile square bottle with a capacity of 250mL (the medium is compos...

Embodiment 3

[0038] Utilize the class Bacillus brevus of embodiment 1 to carry out fermentation, obtain petroleum hydrocarbon degrading bacterium product, concrete fermentation method is carried out according to following steps:

[0039] 1) Streak-inoculate the Bacillus brevis preserved in glycerol at -70°C on the solid medium in the pre-sterilized petri dish in the ultra-clean workbench. The medium is composed of the following components according to the mass percentage: yeast extract 0.6%, peptone 1.2%, agar powder 2%, the balance is water, pH 7.2. The culture dish was placed upside down in an incubator at 33° C. for 2 days to obtain a single colony of activated Bacillus pseudobrevii.

[0040] 2. Pick a single colony and suspend it in 20ml sterilized distilled water by aseptic operation. Sterilize the pipette to draw 1ml and inoculate it on the inclined surface of the solid medium in a sterile square bottle with a capacity of 250mL, and shake the square bottle gently to spread the singl...

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Abstract

The invention discloses a petroleum hydrocarbon degrading bacterium and a fermentation method thereof. The petroleum hydrocarbon degrading bacterium is brevibacillus parabrevis with strain name of GWN-2, and is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No. 13903. The degrading bacterium is characterized in that the gene sequence is shownin a sequence table. After inoculation and proliferation culture of the petroleum hydrocarbon degrading bacterium, a petroleum hydrocarbon degrading bacterium product capable of degrading oily sludgeis obtained, the oil content is reduced by about 80% after the oily sludge is treated for 20 days, and a novel approach is provided for treatment of the oily sludge in oil fields.

Description

technical field [0001] The invention relates to a sludge-removing bacillus fermentation production method, which is especially suitable for the treatment of oily sludge produced in fields such as crude oil exploitation and treatment in oilfields, oily cuttings produced in drilling processes, and ground oil, and belongs to the field of biotechnology. Background technique [0002] Oily sludge is a solid waste composed of crude oil, water, heavy metals, and sludge produced in the process of oil extraction, oil and gas gathering and transportation, refining and processing, and oily sewage treatment. In addition to a large amount of residual oil, the sludge also contains toxic substances such as benzene series, anthracene, phenols, and pyrene, as well as toxic and harmful substances such as heavy metals, polychlorinated biphenyls, dioxins, and radioactive elements. On the one hand, oil entering the soil changes the composition and structure of soil organic matter, causing changes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20C12R2001/01C12N1/205
Inventor 林刚郭鹏庄建全纪艳娟
Owner CHINA PETROLEUM & CHEM CORP
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