A method for preparing bacteriophage Bdellovibrio preparation by enrichment culture
A technology of phage Vibrio and enrichment culture, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of limited lysis ability and insignificant application effect of various pathogenic bacteria
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[0028] A kind of enrichment culture method prepares the method for phage Bdellovibrio preparation, concrete steps are as follows:
[0029] Step 1: Isolation of host bacteria: Take 0.1 ml of water 20 cm below the water surface of seawater shrimp ponds in the middle stage of culture, inoculate it into TCBS Vibrio selective medium, spread it evenly, and place it in a constant temperature incubator at 35°C for upside-down culture 24 hours; the blue-green colonies that appear are Vibrio parahaemolyticus; pick a single colony of Vibrio parahaemolyticus and inoculate it continuously on a TCBS plate for 3 to 5 times, until the microscopic observation shows that the bacteria have the same shape and the same colony shape Bacterial colonies, inoculated in NB test tubes and cultured for 24 hours, are the host bacterial strains of Vibrio parahaemolyticus in this preparation;
[0030] The cultivation conditions of the TCBS Vibrio selective medium are as follows: the controlled temperature i...
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