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Gold nanomaterial as well as preparation method and application thereof

A technology of gold nanometer and gold nanocluster, which is applied in the field of gold nanomaterials, can solve the problem of low activity and achieve the effects of widening application, mild reaction conditions and low biological toxicity

Active Publication Date: 2018-12-28
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these particles often have the defect of low activity

Method used

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  • Gold nanomaterial as well as preparation method and application thereof
  • Gold nanomaterial as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] This example is used to illustrate the synthesis method of the multifunctional fluorescent gold nanocluster provided by the present invention.

[0062] Method 1: Mix 300 μl of 120 mM auric acid chloride (HAuCl 4 ), 10ml of different ratios and different ligands (including thiol quaternary ammonium salt (QA), polyarginine (R9), mercaptopyrimidine (DAPT), cysteine, lysozyme and other thiol ligands and proteins) and 10ml of glutathione (GSH) with a concentration of 3mM was used to obtain a mixed solution, and the solvent was deionized water. The mixture was heated at 70°C for 24 hours to synthesize various functionalized modified fluorescent gold nanoclusters (AuNCs).

[0063] Method 2: Mix 10 μl of 100 mM chloroauric acid (HAuCl4), 20 μl of different ratios (the molar mass ratio of chloroauric acid is 1:1, 1:2, 1:5, etc.) and different ligands (including thiol quaternary Ammonium salt (QA), polyarginine (R9), mercaptopyrimidine (DAPT), cysteine, lysozyme and other sulfh...

Embodiment 2

[0067] This example is used to study the physicochemical properties of gold nanoclusters with different ligands.

[0068] 1. Use a fluorescence spectrometer (model RF-5301PC purchased from Japan Shimadzu Company) to test the fluorescence of the synthetic material.

[0069] 2. Use an inductively coupled plasma optical emission spectrometer (ICP-OES) (instrument model Optima 5300DV, purchased from Pekin-Elmer, USA) to measure the gold element content (μg / ml) of gold nanoclusters used in antibacterial experiments.

[0070] 3. Use a nanoparticle size and Zeta potential analyzer (NZS) (instrument model Zetasizer Nano ZS, purchased from Malvern Instrument Co., Ltd., UK) to measure the size and potential of the material.

[0071] 4. Use a lanthanum hexaboride transmission electron microscope (T-20) (instrument model Tecnai G2 20S-TWIN, purchased from FEI, USA) to observe the appearance of gold nanoclusters.

Embodiment 6

[0115] This test example is used to test the effect of different ligand-modified gold nanocluster materials synthesized in Example 1 on the regulation of bacterial growth in vivo.

[0116] Firstly, a mouse intraperitoneal bacterial infection model was constructed:

[0117] 1. Cultivate the bacteria to the logarithmic phase, collect the bacteria by centrifugation, wash twice with normal saline, and then dilute the bacteria to 6×10 with normal saline. 5 CFU / mL.

[0118] 2. Prepare 10% yeast (angie yeast) solution with physiological saline, and sterilize it for use. The bacterial solution and the yeast solution were mixed at a volume ratio of 1:1 as a mouse intraperitoneal injection. Each mouse was intraperitoneally injected with 500 μL of diluted bacterial solution, and the abdomen of the mouse was lightly rubbed to establish a mouse abdominal infection model.

[0119] 3. After half an hour, the first injection of different concentrations of AuNCs saline solution, simple sali...

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Abstract

The invention provides a gold nanomaterial which is a functionally modified gold nano-cluster, and further provides a preparation method and an application of the gold nanomaterial. The preparation method of the gold nanomaterial is simple, reaction conditions are quite mild and include normal temperature, normal pressure, water phase and about neutral pH, and the biological or medical applicationof the gold nanomaterial is facilitated. The material has good adjusting performance for bacteria growth, and can be applied to tumor imaging and treatment. The functional gold nano-cluster is synthesized from different ligands and applied to growth adjustment. The synthesized gold nano-cluster has the adjusting function for growth of multiple bacteria, and because of lower biotoxicity, the biological and medical application of the material is widened; the material is used for tumor detection and treatment by adjusting growth of the bacteria.

Description

technical field [0001] The invention belongs to the field of materials, and in particular relates to a gold nanomaterial, its preparation method and application. Background technique [0002] The incidence of liver cancer in China is relatively high; moreover, many cancers of the digestive system, including bowel cancer and pancreatic cancer, usually metastasize to the liver, and tumor metastasis is one of the most important factors causing cancer death. The liver is a very regenerative organ; if early cancers can be detected in time and intervened (such as resection of the lesion), the survival rate of cancer patients can be greatly improved. [0003] There has always been an inseparable relationship between humans and bacteria. From ancient times to the present, humans have never stopped developing and utilizing bacteria while fighting against pathogenic bacteria. One hundred years ago, researchers discovered that when patients with sarcoma were acutely infected by strept...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K9/14A61K33/24A61P35/00
CPCA61K9/14A61K33/24A61K49/0052A61K49/0056A61K49/0065A61P35/00
Inventor 蒋兴宇谢阳州昀何俊龙
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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