Cucumber hypocotyledonary axis grafting method for stock-scion interaction research

Abstract

The invention relates to a cucumber hypocotyledonary axis grafting method for stock-scion interaction research. The method comprises the steps of scion and stock cultivation, wherein stock and scion seeds are subjected to seedling growing, after scions grow to obtain one leaf and one heart, stock seed leaves are completely unfolded, and when the thicknesses of stock and scion hypocotyledonary axesare kept consistent, grafting is performed; grafting, wherein a 30-degree slope is cut out at the position 1 cm below scion seed leaves, all stock seed leaves are removed, one cut is formed at the position 1 cm below the stock seed leaves, the section length and the scions are consistent, the scions and the stock section are aligned and tightly attached and fixed through a grafting clamp; original stock pumpkin leaves are partially grafted to an original scion cucumber section, and fixed through a grafting clamp, and stock-scion grafting is performed; post-grafting management, wherein grafting seedlings are placed into a seedling growing bag, a transparent plastic top cover is sufficiently wetted by a sprinkling can, a seedling growing substrate is watered, and the humidity of the small environment inside the seedling growing bag is kept; the seedling growing bag is sealed with a heat preservation film and placed into a 28+ / -2 DEG C cultivation box for dark cultivation for 2-3 d, then, weak light cultivation is performed for 3-4 d, and normal illumination cultivation is performed.

Description

technical field [0001] The invention relates to a cucumber hypocotyl grafting method used for the research on rootstock-ear interaction. Background technique [0002] Cucumber (Cucumis sativus L.) is the main vegetable crop under protected cultivation in northern my country. In 2017, the total cultivated area reached 155,840 hectares, accounting for 58.7% of the world's total cultivated area, and the output was 59.38 million tons, ranking first in the world (FAO, 2017). Solar greenhouses and plastic greenhouses provide favorable conditions for the cultivation of cucumbers, but cucumbers have shallow root systems, which are prone to adversity damage such as soil continuous cropping obstacles, high temperature and low temperature stress, and soil salinization, resulting in reduced yields or even extinction (Kang Shaozhong, 1998). [0003] Grafting is an effective means to overcome soil continuous cropping obstacles, improve plant stress resistance, and prevent and control vari...

AI Technical Summary

Problems solved by technology

The grafting method is to join the scion cucumber seedlings with roots and the rootstock pumpkin seedlings in the middle and upper part of the melon seedlings, and then join them together. Because of the grafting with roots, the cucumber roots need to be cut off after survival, which is time-consuming; The direction of material and information transport between cotyledon and grafted cucumber rootstock and ear is chaotic, which is not conducive to the later research and mechanism explanation of rootstock-ear interaction

The sticking method is to use a blade to cut off a cotyledon and the growth point of the rootstock, and then stick to the slope of the scion stem. Compared with the butting method, this method is faster and does not require root removal in the later stage, but it still exists due to the cotyledons left on the rootstock. This leads to the confusion of the direction of material and information transportation between grafted cucumber roots and ears, which is not conducive to the later research and mechanism interpretation of root-ear interactions.

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