Application of astragaloside III in preparation of drugs
A technology of astragaloside IV and medicine, which is applied in the field of application of astragaloside IV in the preparation of medicines, and can solve problems such as side effects of healthy cells
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Embodiment 1
[0049] Example 1 Astragaloside III increases the percentage of NK1.1+
[0050] In order to study the effect of astragaloside IV on NK cell products, the inventors activated the lymphocytes of wild-type mice with astragaloside IV for 48 hours, and analyzed the percentage of NK1.1+ cells by flow cytometry. We observed that astragaloside III increased the proportion of NK1.1+ cells in a significant dose-dependent manner.
[0051] Splenocytes from C57 mice were cultured in astragaloside IV concentrate for 48 hours in vitro. At least one-third of independent experiments showed that astragaloside III enhanced NK cell expansion in vitro. The experimental result is figure 1 : Representative flow cytometry NK1.1+ cells scatter plot. figure 2 : is the action curve of astragaloside III on NK1.1+ expression.
Embodiment 2
[0052] Example 2 The effect of astragaloside III on cell division
[0053] Based on the fact that astragaloside III can increase the percentage of NK cells cultured in vitro, the inventors tried to prove whether the compound can produce NK cells in NK gene knockout mice. Wild C57 mice were injected with anti-mouse NK1.1+48 hours to remove NK cells in their bodies. Splenocytes from NK-deficient mice were activated with astragaloside III for 48 hours, and the percentages of NK cells and NKT cells were analyzed by flow cytometry. It was observed that astragaloside IV greatly promoted the proliferation of NK cells. NK cells were enriched and stained with CFSE. NK cells were treated with astragaloside Ⅲ for 48 hours, and then the division of NK cells was analyzed by flow cytometry. We observed that astragaloside III can promote NK cell division.
[0054] Splenocytes from NK cell-deficient mice were activated with 100 nM astragaloside III for 48 hours. Astragaloside IV can pr...
Embodiment 3
[0055] Example 3 The Anticancer Effect of Astragaloside IV
[0056] The treatment of tumors by NK cells varies with tumor cells. In order to confirm the therapeutic effect of astragaloside Ⅲ, its effects on osteosarcoma U20S cells and B16 melanoma cells were compared. Osteosarcoma U20S cells and B16 melanoma cells co-cultured with NK cells were activated with astragaloside IV for 6 hours overnight. Tumor cells were analyzed by MTT. It was observed that the inhibitory effect of astragaloside Ⅲ on B16 melanoma cells was stronger than that on osteosarcoma U20S cells. B16 melanoma cells were stained with CFSE and co-cultured with NK cells, and treated with astragaloside IV for 6 hours overnight. It was observed that astragaloside IV could inhibit the proliferation of B16 melanoma cultured by NK cells.
[0057] In vitro therapeutic effect of astragaloside IV on different cancer cells. NK cell-deficient C57 mice were cultured overnight with astragaloside IV. Different tumor c...
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