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A kind of acidic macadamia nut glycoprotein and its production method

A technology of acidic glycoprotein and production method, which is applied in the field of acidic macadamia glycoprotein and its production, can solve the problems of unmentioned residue application, achieve good application prospects, high purity, and improve the effect of resource utilization

Active Publication Date: 2021-09-21
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Patent documents CN 103652313 A, CN 103719531 A, and ZL201410522940.7 all relate to methods for extracting and producing protein or (and) polysaccharides from macadamia nut oil meal and water after low-temperature oil extraction, but after obtaining the above products, there are still 30 ~50% (W / W, based on oil meal) residues, the application of these residues is not mentioned in these technologies

Method used

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  • A kind of acidic macadamia nut glycoprotein and its production method
  • A kind of acidic macadamia nut glycoprotein and its production method
  • A kind of acidic macadamia nut glycoprotein and its production method

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Take 250Kg of shelled macadamia nuts without insects and rot, and about 100Kg of dried and shelled nuts, and about 25Kg of oily meal after oil degreasing in a press; air-dry the oily meal and crush it through a 40-mesh sieve , and then soaked in about 200Kg water for about 1 hour, quickly heated to boiling, and took out the extract after maintaining for 1 hour; the residue was extracted twice according to this method, after collecting the above three extracts, adjust the pH to 4.3, and stand still to obtain the precipitated protein. After centrifugation, precipitation collection and drying, the protein product is obtained. The supernatant after protein precipitation is adjusted to neutral pH, then concentrated by vacuum evaporation at 60°C to a water content below 70%, and then spray-dried or freeze-dried to obtain neutral polysaccharides.

[0051] Add about 8 times (w / v) soda aqueous solution with pH 13 to the remaining residue of the above extraction, heat to boiling ...

Embodiment 2

[0053] Take about 25Kg of macadamia nut oil meal; air-dry the oil meal and crush it through an 80-mesh sieve, let it stand and soak for 1 hour, then heat it to boiling with about 750Kg of water, keep it for 10 minutes, take it out after cooling, and separate the extract; repeat the method for the residue Extract twice, collect all the extracts, adjust the pH to 4.5 until turbidity appears, then centrifuge (<4000rpm), collect the precipitate, and dry to obtain the protein product. The supernatant after protein precipitation is adjusted to neutral pH with soda, then concentrated by vacuum evaporation at 60°C to a water content below 70%, and then spray-dried or freeze-dried to obtain neutral polysaccharides.

[0054] Then add about 20 times (w / v) sodium bicarbonate aqueous solution of pH 10 to the residue after the above extraction, heat to boiling and keep warm for 2 hours to obtain an alkaline extract containing glycoprotein; The extraction was repeated twice; the glycoprotein...

Embodiment 3

[0056] Take 25Kg of oil residue from a macadamia nut company in Yunnan, which uses low-temperature supercritical carbon dioxide as a solvent to extract oil. After drying, crush and pass through a 20-mesh sieve, heat it to boiling with about 300Kg of water and keep it for 0.5h. After cooling, take it out and separate it for extraction. solution; the residue was extracted twice according to this method, and after collecting all the extracts, neutral polysaccharides and proteins were obtained according to the method of Example 1. Then add about 20 times (w / v) sodium bicarbonate aqueous solution of pH 8 to the residue, heat to boiling and keep warm for 1.5h to obtain an alkaline extract containing glycoprotein; then repeat the extraction of the residue 2 times according to the above method; The glycoprotein extracts were collected three times, and after the residue was removed by centrifugation, the acid glycoprotein was obtained according to the method described in Example 1, with...

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Abstract

The invention provides an acidic macadamia nut glycoprotein and a production method thereof. The production method mainly includes adding water to the degreased oil residue and boiling it, adding an alkali solution to the precipitate to extract the glycoprotein, separating the acidic protein through the acidity gradient precipitation separation technology, and then dehydrating and drying to obtain the described macadamia acid sugar protein. The method makes full use of the macadamia oil meal resource, has simple production process, easy operation of the purification method, and the product purity is as high as over 96%, which further improves the resource utilization rate and reduces waste discharge. The invention also provides a glycoprotein product, which has biological activities such as anti-oxidation and multiplication of probiotics. At the same time, it also provides a co-production method of macadamia nut protein, neutral polysaccharide and acid glycoprotein, which can easily and efficiently obtain three products of macadamia nut protein, neutral polysaccharide and acid glycoprotein from defatted oil meal, and the process is simple , easy to produce and promote, and has good application prospects.

Description

technical field [0001] The invention belongs to the field of deep processing of agricultural products, and in particular relates to an acidic macadamia nut glycoprotein and a production method thereof. Background technique [0002] Macadamia ternifolia F.Muell, also known as macadamia nut, Australian walnut, Queensland fruit, etc., is a dicotyledonous evergreen tree of the genus Macadamia of the family Proteaceae, native to Queensland and New South Wales, Australia subtropical rainforest. my country has cultivated more than 3 million mu of macadamia nuts, and the scale of cultivation will increase year by year in the next five years. Macadamia nuts are rich in nutrition and have a unique and elegant flavor, known as the "Queen of Dried Fruits". [0003] Nuts are mainly used as dried fruits, because of their high oil content (accounting for about 80% of the weight of the nuts), their oils have nutritional and health effects such as anti-oxidation, diabetes prevention, preve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/14C08B37/00C07K1/14C07K1/30
CPCA23J1/14C07K1/145C07K1/30C08B37/00C08B37/0003
Inventor 黄雪松王文林陈海生郑树芳谭秋锦黄锡云
Owner JINAN UNIVERSITY
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