Acidic Macadamia nut glycoprotein and production method thereof

A technology of acid glycoprotein and production method, applied in the field of acid macadamia nut glycoprotein and production thereof, can solve problems such as the application of residues not mentioned, and achieve the effects of good application prospect, simple production process, and no waste of resources

Active Publication Date: 2018-12-21
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Patent documents CN 103652313 A, CN 103719531 A, and ZL201410522940.7 all relate to methods for extracting and producing protein or (and) polysaccharides from macadamia nut oil meal and water after low-temperature oil extraction, but after obtaining the above products, there are still 30 ~50% (W / W, based on oil meal) residues, the application of these residues is not mentioned in these technologies

Method used

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  • Acidic Macadamia nut glycoprotein and production method thereof
  • Acidic Macadamia nut glycoprotein and production method thereof
  • Acidic Macadamia nut glycoprotein and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Take 250Kg of macadamia nuts without insects and rot in the shell. After drying and dehulling, the kernels are about 100Kg. After the oil is defatted by a press, about 25Kg of oil meal is obtained; the oil meal is air-dried and crushed through a 40-mesh sieve , Then soak in about 200Kg of water for about 1 hour, quickly heat to boiling, and then take out the extract after maintaining for 1 hour; the residue is extracted twice according to this method, after collecting the above three extracts, adjust the pH to 4.3, and let it stand to get the precipitated protein. After centrifugation, collection and drying, the protein product is obtained. The supernatant after protein precipitation, after adjusting the pH to neutral, is concentrated by vacuum evaporation at 60°C to a water content of less than 70%, and spray-dried or freeze-dried to obtain neutral polysaccharides.

[0051] Add about 8 times (w / v) pH 13 soda solution to the remaining residue of the above extraction, heat ...

Embodiment 2

[0053] Take about 25Kg of macadamia nut oil meal; dry the oil meal and pulverize it through an 80-mesh sieve, let it stand for 1 hour, and then heat it with about 750Kg of water to boiling for 10 minutes, after cooling, take out and separate the extract; the residue is repeated according to the method Extract twice. After collecting all the extracts, adjust the pH to 4.5 until turbidity appears, and then centrifuge ( <4000rpm), the protein product obtained after collecting the precipitate and drying. After precipitating the protein, the supernatant is adjusted to neutral pH with soda, and then concentrated by vacuum evaporation at 60°C to a water content of less than 70%. After spray dehydration or freeze drying, neutral polysaccharides are obtained.

[0054] Then add about 20 times (w / v) of a pH 10 aqueous sodium bicarbonate solution to the residue after the above extraction, heat it to boiling and keep it for 2 hours to obtain an alkaline extract containing glycoproteins; then a...

Embodiment 3

[0056] Take 25Kg of oil dregs from a macadamia nut company in Yunnan that uses low-temperature supercritical carbon dioxide as a solvent to extract oil. After drying, pulverize through a 20-mesh sieve, and then use about 300Kg of water to heat to boiling for 0.5h. After cooling, take it out to separate and extract. The residue was extracted twice according to this method. After collecting all the extracts, neutral polysaccharides and proteins were obtained according to the method of Example 1. Then add about 20 times (w / v) of the sodium bicarbonate solution of pH 8 to the residue, heat it to boiling and keep it for 1.5 hours to obtain an alkaline extract containing glycoprotein; then repeat the extraction of the residue twice according to the above method; The glycoprotein extract was collected three times, and after centrifugation to remove the residue, acid glycoprotein was obtained according to the method described in Example 1, with a yield of 2.4% (w / w).

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Abstract

The invention provides an acidic Macadamia nut glycoprotein and a production method thereof. The production method mainly comprises the steps of adding water into defatted oil meal, carrying out boiling, adding an alkaline solution into precipitates to extract glycoprotein, separating acidic protein by an acidity gradient precipitation separating technology, and then, carrying out dewatering and drying, thereby obtaining the acidic Macadamia nut glycoprotein. According to the method, oil meal resources of Macadamia nuts are fully utilized, the production process is simple, the purification method is easy to operate, the product purity reaches up to 96% or more, the utilization ratio of resources is further increased, and waste discharge is also reduced. The invention further provides a glycoprotein product which has the bioactivity of resisting oxidation, propagating probiotics and the like. Simultaneously, the invention further provides a coproduction method of Macadamia nut proteins,neutral polysaccharides and acid glycoproteins. Three kinds of products, i.e., the Macadamia nut proteins, the neutral polysaccharides and the acid glycoproteins can be simply, conveniently and efficiently obtained from the defatted oil meal, the process is simple, production and popularization are facilitated, and thus, the method has a good application prospect.

Description

Technical field [0001] The invention belongs to the field of deep processing of agricultural products, and particularly relates to an acidic macadamia nut glycoprotein and a production method thereof. Background technique [0002] Macadamia (Macadamia ternifolia F.Muell), also known as macadamia nuts, macadamia nuts, queensland nuts, etc., is a dicotyledonous evergreen tree of the Proteaceae family (Proteaceae), macadamia (Macadamia), native to Queensland, Australia and New South Wales Subtropical rain forest. my country has cultivated more than 3 million mu of macadamia nuts, and the scale of cultivation will increase year by year in the next five years. Macadamia nuts are rich in nutrients and have a unique and elegant flavor. They are called the "Queen of Dried Fruits". [0003] In addition to being mainly used for eating dried fruits, nuts have high oil content (approximately 80% of the weight of nuts), and their oils have anti-oxidant, prevent diabetes, prevent and reduce ca...

Claims

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Application Information

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IPC IPC(8): A23J1/14C08B37/00C07K1/14C07K1/30
CPCA23J1/14C07K1/145C07K1/30C08B37/00C08B37/0003
Inventor 黄雪松王文林陈海生郑树芳谭秋锦黄锡云
Owner JINAN UNIVERSITY
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