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A kind of method utilizing xylose as raw material to produce ethylene glycol and glycolic acid

A technology of xylose acid and ethylene glycol, which is applied in the field of bioengineering to achieve the effects of high production intensity, high substrate conversion rate, and tenacious growth of bacteria

Active Publication Date: 2021-06-25
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, there is no report on the production of glycolic acid and ethylene glycol by using wild-type microorganisms. The common way of biosynthesizing glycolic acid and ethylene glycol is to use Escherichia coli, Corynebacterium glutamicum or other microorganisms as hosts. Construct ethylene glycol or glycolic acid synthesis pathway, use xylose or glucose as carbon source to ferment and produce glycolic acid and ethylene glycol

Method used

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  • A kind of method utilizing xylose as raw material to produce ethylene glycol and glycolic acid

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Effect test

Embodiment 1

[0027] Production of xylonic acid from xylose by conversion of xylose by Lebsiella pneumoniae.

[0028] Klebsiella pneumoniae was inoculated into a 250mL Erlenmeyer flask containing 50mL of seed medium, and the shaker cabinet was rotated at 200 rpm and kept at a constant temperature of 35°C for seed cultivation.

[0029] The components of the seed medium are: peptone 10g / L, yeast extract 5g / L, sodium chloride 5g / L.

[0030] The composition of the fermentation medium is: xylose 50g / L, corn steep liquor 50g / L, potassium dihydrogen phosphate 3g / L, dipotassium hydrogen phosphate 2g / L, calcium chloride 1g / L, magnesium sulfate 4g / L.

[0031] After the seeds were cultured for 12 hours, they were inoculated into a 5L fermenter with 3L of fermentation medium, and the dissolved oxygen concentration during the fermentation process was kept greater than 1% of the saturated dissolved oxygen concentration. The fermentation temperature was 35°C, the pH was controlled at 6, and cultured for 5...

Embodiment 2

[0033] Glucose oxidase and catalase are used as composite catalysts to catalyze the conversion of xylose to produce xylonic acid.

[0034] Glucose oxidase was added to the 5L reaction at an enzyme activity ratio of 2000 units per kilogram of xylose, and catalase was contained in 3L of 10g / L xylose solution. The reaction temperature was 25°C, the conversion process was ventilated with air and stirred, the pH value of the conversion process was maintained at 4.5 by using ammonia water, and the conversion was completed in 5 hours to obtain 10 g / L xylonic acid.

[0035] The obtained xylonic acid solution is used as raw material for the subsequent production of glycolic acid and ethylene glycol.

Embodiment 3

[0037] Glucose oxidase and catalase are used as composite catalysts to catalyze the conversion of xylose to produce xylonic acid. Glucose oxidase and catalase are industrial grade products.

[0038] Glucose oxidase is added to the 5L reaction at an enzyme activity ratio of 50,000 units per kilogram of xylose, and catalase is contained in 2L of 500g / L xylose solution. The reaction temperature was 50°C, the conversion process was aired and stirred, and the pH value of the conversion process was maintained at 7 by using sodium hydroxide solution. After 150 hours of conversion, a 480 g / L xylonic acid solution was obtained.

[0039] The obtained xylonic acid solution is used as raw material for the subsequent production of glycolic acid and ethylene glycol.

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Abstract

The invention discloses a method for producing ethylene glycol and glycolic acid by using xylose as a raw material. In this method, xylose is used as a raw material to generate xylose acid by enzymatic conversion method or fermentation conversion method, and enterobacter cloacae and Escherichia coli are used as substrates to ferment xylose acid under neutral pH and aerobic conditions to produce glycolic acid and ethylene glycol. The two production strains adopted in the invention are wild-type bacteria with high genetic stability and tenacious growth. The production of glycolic acid and ethylene glycol by these two bacterial strains has the advantages of simple production process, high substrate conversion rate, low by-product content, etc., and has good application value and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to converting xylose into xylonic acid, and then using enterobacter cloacae or Escherichia coli as a production strain to produce glycolic acid and ethylene glycol by using xylonic acid as a carbon source. Background technique [0002] Xylose is the second most abundant monosaccharide in nature after glucose, and its main application is as a raw material for the production of furfural. Xylose can also be used as a carbon source for microbial growth, but the growth of most microorganisms using xylose as a carbon source is not as good as using glucose. How to efficiently utilize xylose is a research hotspot in the world. Xylonic acid is an oxidation product of xylose, and its main application is as a raw material for the production of esters and as a chelating agent. [0003] Glycolic acid, also known as glycolic acid, is an alpha hydroxy acid with a small structur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/58C12P7/18C12P7/42C12R1/22C12R1/19C12R1/01
CPCC12P7/18C12P7/42C12P7/58
Inventor 郝健张忠喜史吉平姜标
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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