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A kind of porcine circovirus type 2 strain, vaccine composition and its preparation method and application

A technology for porcine circovirus and vaccine composition, applied in the field of animal virology, can solve problems such as protection of incapable strains, and achieve the effects of reducing production costs, low antigen content, and good immune protection.

Active Publication Date: 2022-05-24
PU LIKE BIO ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the prevalence of this new PCV2 gene subtype strain, and the difference between its antigen and the existing genotype, and the existing commercial vaccines are all prepared with PCV2a or PCV2b as the vaccine strain, it cannot be used for the latest Therefore, it is very important to prepare new vaccines against the latest circulating strains for disease control in swine farms

Method used

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  • A kind of porcine circovirus type 2 strain, vaccine composition and its preparation method and application
  • A kind of porcine circovirus type 2 strain, vaccine composition and its preparation method and application
  • A kind of porcine circovirus type 2 strain, vaccine composition and its preparation method and application

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Experimental program
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Embodiment approach

[0028] As an embodiment of the present invention, the antigen of the porcine circovirus type 2 HH3 strain is a whole virus inactivated antigen of the porcine circovirus type 2 HH3 strain or its culture.

[0029] As an embodiment of the present invention, the antigen of the porcine circovirus type 2 HH3 strain is a split antigen of the porcine circovirus type 2 HH3 strain or a culture thereof.

[0030] The gene sequences of different generations and sub-cultures of the virus may only have slight variations, which can ensure the same immunogenicity, and the inactivated vaccines prepared by them can have similar immune efficacy.

[0031] As an embodiment of the present invention, the porcine circovirus type 2 HH3 strain culture is a culture of ≥1 passage.

[0032] As a preferred embodiment of the present invention, the porcine circovirus type 2 HH3 strain culture is a culture of ≥5 passages.

[0033] As a more preferred embodiment of the present invention, the culture of the por...

Embodiment 1

[0074] Example 1 Isolation and identification of porcine circovirus type 2

[0075] 1. Source of disease materials

[0076] In a domestic pig farm that was immunized with commercialized PCV2 vaccine, there were sporadic occurrences of miscarriage and increased mummified fetuses in sows. Affected sows exhibited symptoms of anorexia and aborted litters containing mummified fetuses of various gestational ages, consistent with symptoms of PCV2-related abortion. PCV2 was positive by immunohistochemistry and quantitative PCR. PCV2 was still detected in the affected pig tissue after immunization with the commercial PCV2 vaccine. The reason is worthy of further investigation. In order to further find out the reason, the disease materials of each tissue were selected for pathogen isolation.

[0077] 2. Isolation and culture of virus strains

[0078] The diseased material was added to DMEM culture medium at 1:10 (volume ratio), ground, and tissue suspension was prepared; after repeat...

Embodiment 2

[0082] Example 2 Screening of porcine circovirus type 2 vaccine strains

[0083] Specific primers were designed according to the porcine circovirus type 2 isolated above, and 42 suspected PCV2-positive samples collected from pig farms with sporadic disease after immunization with commercialized PCV2 vaccine were analyzed by quantitative PCR, and 16 strains of PCV2 virus were screened and isolated. Among these 16 strains, the homology of the nucleotide sequence of the genome is as high as 99.6-100%, and the homology of the amino acid sequence of the Cap protein is as high as 99.5-100%. Further analysis belongs to the same genotype. Through the animal pathogenicity test and immunogenicity test, a PCV2 virus strain with strong pathogenicity, good immunogenicity and broad-spectrum was finally screened. The porcine circovirus type 2 virus was named as porcine circovirus type 2 HH3 strain and submitted for deposit.

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Abstract

The invention relates to a strain of a new gene subtype of porcine circovirus type 2, which has good and broad-spectrum immunogenicity. The prepared vaccine composition can prevent and / or treat related diseases caused by porcine circovirus type 2 infection, and can have good immune protection effect against strains from different regions.

Description

technical field [0001] The invention relates to a virus strain, a vaccine composition prepared by the same, a preparation method and application, and belongs to the field of animal virology. Background technique [0002] Porcine circoviruses (PCVs) are single-stranded circular DNA viruses with a genome length of about 1.7 kb, which is one of the smallest animal DNA viruses. Two types of PCV have been identified, porcine circovirus type 1 (PCV1) and porcine circovirus type 2 (PCV2). PCV1 was first identified as a contaminant in PK cell cultures in 1974 and was not pathogenic in pigs. PCV2, which was first reported in 1998, can cause porcine circovirus associated diseases (PCVAD) in pigs under clinical conditions, mainly causing multisystem wasting syndrome, pneumonia, porcine dermatitis and nephrotic syndrome in weaned piglets and reproductive disorders, mainly manifested as respiratory, urinary, intestinal, lymphatic, cardiovascular, nervous, reproductive system and skin d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/12A61K39/187A61K39/295A61K39/245A61K39/23A61K39/102A61K39/02A61K39/116A61P31/20A61P31/14A61P31/22A61P31/04C12R1/93
CPCA61K39/0241A61K39/102A61K39/12C12N7/00A61K2039/70A61K2039/552A61K2039/5252C12N2710/16734C12N2770/10034C12N2750/10021C12N2750/10034C12N2750/14034C12N2770/24334
Inventor 田克恭姚亚丽孙进忠张许科
Owner PU LIKE BIO ENG
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