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Preparation method for injectable poly(L-lactic acid) microsphere

A technology of L-polylactic acid and microspheres, which is applied in the field of medical cosmetic materials, can solve problems such as poor redissolving and dispersibility of polylactic acid microspheres, and achieve the effect of good effect and simple process steps.

Active Publication Date: 2018-12-18
普丽妍(南京)医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Purpose of the invention: To solve the problem of poor reconstitution and dispersibility of polylactic acid microspheres prepared by existing methods, the present invention provides a preparation method for injectable L-lactic acid microspheres, which can obtain L-polylactic acid microspheres with good reconstitution and dispersibility

Method used

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  • Preparation method for injectable poly(L-lactic acid) microsphere

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Dissolve PLLA with an intrinsic viscosity of 1.5g / dL in chloroform to form a solution with a concentration of 1g / L; dissolve sodium carboxymethylcellulose and mannitol (mass ratio 1:20) with a molecular weight of 500,000Da in distilled water , dubbed a solution with a concentration of 0.5%. The chloroform solution of PLLA was slowly added dropwise to the sodium carboxymethylcellulose / mannitol aqueous solution under stirring, and the volume ratio of the two solutions was 1:100. Stirring is continued for 12 hours, and the mixed solution is vacuumed by an oil pump for 5 hours, and then the solution is freeze-dried to obtain injectable poly-L-lactic acid microspheres; the yield of the injectable poly-L-lactic acid microspheres is 80%.

[0020] Dissolve the freeze-dried poly-L-lactic acid microspheres in water, and after the water is air-dried, obtain its scanning electron micrograph, as shown in figure 1 , It can be seen that the L-polylactic acid microspheres prepared in ...

Embodiment 2

[0022] Dissolve PLLA with an intrinsic viscosity of 0.1 g / dL in chloroform to form a solution with a concentration of 100 g / L. Sodium carboxymethyl cellulose with a molecular weight of 5000 Da and mannitol (20:1 by mass ratio) were dissolved in distilled water to prepare a solution with a concentration of 5 wt%. The chloroform solution of PLLA was slowly added dropwise to the sodium carboxymethylcellulose / mannitol aqueous solution under stirring, and the volume ratio of the two solutions was 1:5. Stirring was continued for 36 hours, and the mixed solution was vacuum pumped by an oil pump for 3 hours, and then the solution was freeze-dried to obtain injectable poly-L-lactic acid microspheres; the yield of the injectable poly-L-lactic acid microspheres was 73%.

[0023] After it was freeze-dried, it was dissolved in water, and the obtained scanning electron micrograph and figure 1 similar.

Embodiment 3

[0025] Dissolve PLLA with an intrinsic viscosity of 0.8 g / dL in chloroform to form a solution with a concentration of 10 g / L. Sodium carboxymethyl cellulose with a molecular weight of 100,000 Da and mannitol (mass ratio: 1:10) were dissolved in distilled water to prepare a solution with a concentration of 2.5 wt%. The chloroform solution of PLLA was slowly added dropwise into the aqueous solution of sodium carboxymethylcellulose / mannitol under stirring, and the volume ratio of the two solutions was 1:20. Stirring is continued for 48 hours, the mixed solution is vacuumed by an oil pump for 0.5 hour, and the solution is freeze-dried to obtain injectable poly-L-lactic acid microspheres; the yield of the injectable poly-L-lactic acid microspheres is 90%.

[0026] After it was freeze-dried, it was dissolved in water, and the obtained scanning electron micrograph and figure 1 similar.

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Abstract

The invention discloses a preparation method for an injectable poly(L-lactic acid) microsphere. The preparation method comprises the following steps: (1) preparing an organic solution of poly(L-lacticacid); (2) preparing an aqueous solution of sodium carboxymethylcellulose / mannitol; (3) adding the organic solution of poly(L-lactic acid) into the aqueous solution of sodium carboxymethylcellulose / mannitol drop by drop, carrying out uniform mixing, and then removing an organic solvent in the mixed solution; and (4) lyophilizing the solution obtained in the step (3) so as to obtain the injectablepoly(L-lactic acid) microsphere. According to the preparation method of the invention, a mixed solvent of sodium carboxymethylcellulose and mannitol is used as a stabilizer; when the freeze-dried mixture is redissolved, the poly(L-lactic acid) microsphere can be uniformly dispersed in the solution, and good injectable effect is obtained; and the preparation method of the invention is simple in process steps and suitable for mass preparation and industrial application.

Description

technical field [0001] The invention relates to a preparation method of injectable L-lactic acid microspheres, belonging to the technical field of medical cosmetic materials. Background technique [0002] Biodegradable polymer materials represented by polylactic acid and its copolymers have been widely used in the fields of absorbable medical devices, tissue engineering, drug controlled release, and medical cosmetology due to their good biocompatibility and biodegradability. The degradation product of L-lactic acid in the body is L-lactic acid, which is an intermediate product of the tricarboxylic acid cycle in the process of carbohydrate metabolism in the body, and can be completely metabolized by the enzymes in the body into carbon dioxide and water to be excreted from the body. Microspheres based on polylactic acid biodegradable polymer materials and their copolymers, as drug controlled release carriers, have been widely used in targeted nano-drug delivery and sustained a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/58
Inventor 何斌蒲雨吉张译心张曦丹谢家银
Owner 普丽妍(南京)医疗科技有限公司
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