Preparation method of double-layer artificial small-diameter blood vessel with modified inner layer

A small-diameter, inner-layer technology, applied in the manufacture of rayon, single-component polyurethane rayon, conjugated synthetic polymer rayon, etc. Compatibility and degradation performance, no inflammatory reaction, good blood compatibility

Active Publication Date: 2021-01-29
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report of using electrospinning technology to prepare inner and outer layers with different degradation rates, and the inner layer is modified with MSN-PEG-Heparin.

Method used

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  • Preparation method of double-layer artificial small-diameter blood vessel with modified inner layer
  • Preparation method of double-layer artificial small-diameter blood vessel with modified inner layer
  • Preparation method of double-layer artificial small-diameter blood vessel with modified inner layer

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Effect test

Embodiment 1

[0028] A method for preparing a composite double-layer artificial small-caliber blood vessel with an inner layer modified by MSN-PEG-Heparin:

[0029] (1) Ultrasonic disperse 40 mg of carboxylated mesoporous silicon (MSNs-COOH) into 20 mL of 0.01N NaOH, stir for 10 minutes and centrifuge for 20 minutes, and wash the MSNs with water for 3 times; then, MSNs are dispersed in 10 mL of 0.05M In the mixed solution of MES and 0.5M NaCl, after stirring for 1 hour, 0.12M EDC, 2mg / mL PEG and 0.06M NHS were added. The mixture solution was stirred at room temperature for 16 hours. The final product was washed with water for 3 times and centrifuged to obtain MSN-PEG;

[0030] (2) Disperse 40 mg of the prepared MSN-PEG in 10 mL of 15% heparin solution and stir for 24 hours. MSN-PEG-Heparin was obtained after centrifugation;

[0031] (3) Disperse the MSN-PEG-Heparin prepared in (2) in HFIP (3mg / mL), then sonicate for 30 minutes to ensure uniform dispersion;

[0032] (4) Prepare the inner...

Embodiment 2

[0036] A method for preparing a composite double-layer artificial small-caliber blood vessel with an inner layer modified by MSN-PEG-Heparin:

[0037] (1) Ultrasonic disperse 40 mg of carboxylated mesoporous silicon (MSNs-COOH) into 20 mL of 0.01N NaOH, stir for 10 minutes and centrifuge for 20 minutes, and wash the MSNs with water for 3 times; then, MSNs are dispersed in 10 mL of 0.05M In the mixed solution of MES and 0.5M NaCl, after stirring for 1 hour, 0.12M EDC, 2mg / mL PEG and 0.06M NHS were added. The mixture solution was stirred at room temperature for 16 hours. The final product was washed with water for 3 times and centrifuged to obtain MSN-PEG;

[0038] (2) Disperse 40 mg of the prepared MSN-PEG in 10 mL of 15% heparin solution and stir for 24 hours. MSN-PEG-Heparin was obtained after centrifugation;

[0039](3) Disperse the MSN-PEG-Heparin prepared in (2) in HFIP (3mg / mL), then sonicate for 30 minutes to ensure uniform dispersion;

[0040] (4) Prepare the inner ...

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Abstract

The invention discloses a preparation method for an inner layer-modified double-layer artificial small-diameter vessel. The preparation method is characterized by comprising the following steps: dispersing carboxylated meso-porous silicon into NaOH to obtain MSN; dispersing the MSN in a mixed solution of MES and NaCl, and adding EDC, PEG and NHS after stirring to obtain MSN-PEG; dispersing the MSN-PEG in heparin solution to obtain MSN-PEG-Heparin; dispersing the MSN-PEG-Heparin in HFIP to obtain dispersion liquid; respectively preparing inner spinning solution and outer spinning solution, andspinning to prepare a vessel stent. The inner layer-modified double-layer artificial small-diameter vessel can be used for revascularization treatment; the preparation method is simple; meanwhile, theinner layer is modified by the MSN-PEG-Heparin, is high in degradation speed, promotes the growth and adhesion of endothelial cells, and is matched with the regenerating speed of neointima; the outerPU is low in degradation speed, so that the prepared tubular stent maintains good mechanical property in vivo for a long time.

Description

technical field [0001] The invention relates to a preparation method of an artificial blood vessel with inner and outer layers with different degradation rates, in particular to a preparation method of an artificial blood vessel with an inner layer modified by MSN-PEG-Heparin. Background technique [0002] Cardiovascular disease is a common disease leading to high mortality. Small-caliber (<6mm) artificial blood vessels have a wide demand in the treatment of coronary and peripheral vascular diseases, but there are still many problems in the application of small-caliber blood vessels. The failure of rapid endothelialization of artificial small blood vessel grafts is likely to cause intimal thickening, vascular stenosis and thrombosis, resulting in low long-term patency rate of small-caliber artificial blood vessels. Research on small-diameter artificial blood vessels mainly focuses on two aspects: one is to improve the hemocompatibility and biocompatibility of stent mater...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/58A61L27/50A61L27/42A61L27/18D01F8/14D01F8/02D01F6/70D01F1/10
CPCA61L27/18A61L27/427A61L27/50A61L27/507A61L27/58D01F1/10D01F6/70D01F8/02D01F8/14C08L67/04C08L5/10C08L89/00C08L75/04
Inventor 莫秀梅匡海珠蒋舟易志文
Owner DONGHUA UNIV
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