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Aged grain fermentation mash for removing aflatoxin, and preparation method thereof

A technology of aflatoxin and fermented mash, applied in the field of food technology, can solve problems such as affecting product quality, threatening feed safety, animal development and human health, economic losses, etc., and achieves the effect of huge economic value and social benefit.

Inactive Publication Date: 2018-12-14
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because aflatoxin is difficult to decompose and remove during ethanol fermentation and distillation, there is still a relatively high concentration of aflatoxin in the final feed solids, which seriously affects product quality, threatens feed safety, animal development and human health, and has caused significant damage to enterprises. Economic losses

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Aged Grain Fermented Mash from Aflatoxin Removal and Preparation Method thereof

[0021] Aged grain fermentation mash for removing aflatoxin and its preparation method, using yeast to ferment aged grains to obtain initial mash, and then undergoing Arthrobacter luteus anaerobic fermentation, ultrasonic wave, high-pressure homogenization, etc. The mash can effectively remove the aflatoxin in it.

[0022] An aged grain fermented liquor from which aflatoxin has been removed and a preparation method thereof comprise the following steps.

[0023] (1) Prepare the starting mash of aged grain

[0024] The aged grain is a mixture of aged corn, aged rice and aged wheat according to the ratio of 1:1:1. After testing, the aflatoxin content is 380 μg / kg.

[0025] Preparation of aged grain liquefaction solution. Dehulling aged grains, crushing, and sieving 55 meshes to obtain aged grain powders; stirring the aged grain powders, water, and potassium chloride according to ...

Embodiment 2

[0040] Embodiment two: A kind of compound enzyme preparation and its preparation method for removing aflatoxin

[0041] A method for preparing aged grain fermented liquor for removing aflatoxin, comprising the following steps:

[0042] The first step: dehulling, crushing, and passing through a 40-mesh sieve to obtain aged grain powder; mix the aged grain powder, water, and potassium chloride evenly in a mass ratio of 100:420:0.3 to obtain starch Suspension, add 2 parts by weight of α-amylase with an activity of 8000 U / g to 1000 parts by weight of starch suspension, then heat to 90°C, stir for 35min, cool to 62°C, and use a concentration of 2 mol / L hydrochloric acid to adjust the pH value to 4 to obtain the aged grain liquefaction solution.

[0043] Step 2: Add 2 parts by weight of glucoamylase with an activity of 45,000 U / g to 1,000 parts by weight of the liquefied liquid of aged grain, and incubate at 55° C. for 40 minutes to obtain the saccharified liquid of aged grain. Ad...

Embodiment 3

[0049] Embodiment three: A kind of compound enzyme preparation and its preparation method for removing aflatoxin

[0050] A method for preparing aged grain fermented liquor for removing aflatoxin, comprising the following steps:

[0051] The first step: dehulling, crushing, and passing through a 50-mesh sieve to obtain aged grain powder; mix the aged grain powder, water, and potassium chloride evenly in a mass ratio of 100:435:0.4 to obtain starch Suspension, add 2.5 parts by weight of α-amylase with an activity of 9000 U / g to 1000 parts by weight of starch suspension, then heat to 2°C, stir for 38min, cool to 64°C, and use a concentration of 3.5 mol / L hydrochloric acid to adjust the pH value to 4.2 to obtain the liquefied liquid of aged grain.

[0052] Step 2: Add 2.5 parts by weight of glucoamylase with an activity of 47,000 U / g to 1,000 parts by weight of the liquefied liquid of aged grain, and incubate at 60°C for 45 minutes to obtain the saccharified liquid of aged grain...

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PUM

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Abstract

The invention relates to an aged grain fermentation mash for removing aflatoxin, and a preparation method thereof. Aged grains are fermented by yeast to obtain a starting mash, and then Arthrobacter luteus anaerobic fermentation, ultrasonic treatment and high pressure homogenization treatment of the starting mash are carried out to effectively remove the aflatoxin. Microbial fermentation, lysozymeexpression, cell wall destroying, catalysis, ultrasonic treatment and high pressure homogenization technologies are integrated against the technologic characteristics of production of ethanol from the aged grains in order to obtain yeast cell wall fragments for aflatoxin, the produced yeast cell wall fragments can efficiently adsorb aflatoxin residual in the mash, and the content of biologicallytoxic aflatoxin in the treated fermentation mash is greatly reduced.

Description

technical field [0001] The invention relates to an aged grain fermented mash from which aflatoxin has been removed and a preparation method thereof, belonging to the technical field of food technicians. Background technique [0002] Aspergillus flavus and Aspergillus parasiticus secrete aflatoxins with dihydrofuranocoumarin structural analogs, which are highly toxic and highly carcinogenic, and belong to food-borne secondary mycotoxins. Aflatoxin poses a huge threat to animal health. After eating feed containing aflatoxin, livestock and poultry will be mildly poisoned chronically, leading to symptoms of decreased immunity, infertility, and digestive mechanism disorders; severe cases will cause symptoms of acute poisoning, and even sudden die. In addition, aflatoxins also pose a huge threat to human health and can easily cause various cancers. Aspergillus flavus and Aspergillus parasiticus are found in the natural environment, stored in soil, animals and plants, and various...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/14A23L5/20C12P7/06C12R1/865
CPCA23K10/12A23K10/14A23L5/273C12P7/06Y02E50/10
Inventor 杨培周姜绍通郑志赵二永肖伟吴芸
Owner HEFEI UNIV OF TECH
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