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Kit and method for detecting heparin binding protein by dry-type quantitative immunofluorescence technology

A heparin-binding protein and immunofluorescence technology, which is applied in the field of heparin-binding protein assay kits and heparin-binding protein assays, can solve the problems of no high-tech, etc., and achieve good stability and repeatability

Inactive Publication Date: 2018-12-07
HANGZHOU JOINSTAR BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] Heparin-binding protein is superior to procalcitonin and C-reactive protein in the diagnosis of infectious diseases, especially for the diagnosis and prognosis of sepsis. Most of the varieties, no high-tech

Method used

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  • Kit and method for detecting heparin binding protein by dry-type quantitative immunofluorescence technology
  • Kit and method for detecting heparin binding protein by dry-type quantitative immunofluorescence technology
  • Kit and method for detecting heparin binding protein by dry-type quantitative immunofluorescence technology

Examples

Experimental program
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Effect test

Embodiment 1

[0050] A heparin-binding protein assay kit, comprising: a detection reaction plate and a detection buffer;

[0051] The detection reaction plate includes: detection test strip, blood filter pad and absorbent paper;

[0052]The detection test strip includes: a nitrocellulose membrane and a carrier bottom plate coated with heparin-binding protein monoclonal antibody, goat anti-rabbit monoclonal antibody;

[0053] Detection buffer solution includes: 8.00g / L NaCl, 0.1g / L Na 2 HPO4, 0.1g / L KH 2 PO4, 0.5% wt PVP, 0.5% wt BSA, 0.05% wt Tween-20, 0.01% wt sodium azide, 0.05% wt proclin300, 0.0008 mg / mL fluorescently labeled heparin-binding protein monoclonal antibody, and 0.00005 mg / mL fluorescent Solution of labeled rabbit monoclonal antibody; assay buffer pH 7.2.

Embodiment 2

[0055] A heparin-binding protein assay kit, comprising: a detection reaction plate and a detection buffer;

[0056] The detection reaction plate includes: detection test strip, blood filter pad and absorbent paper;

[0057] The detection test strip includes: a nitrocellulose membrane and a carrier bottom plate coated with heparin-binding protein monoclonal antibody, goat anti-rabbit monoclonal antibody;

[0058] Detection buffer solution includes: 60.00g / L NaCl, 5.5g / L Na 2 HPO4, 5.5g / L KH 2 Solution of PO4, 5% wt PVP, 5% wt BSA, 1.0% wt Tween-20, 1.0% wt sodium azide, 1.0% wt proclin300, 0.0015 fluorescently labeled heparin-binding protein monoclonal antibody and 0.00015 mg / mL fluorescent Labeled rabbit monoclonal antibody; assay buffer pH 7.2.

Embodiment 3

[0060] A heparin-binding protein assay kit, comprising: a detection reaction plate and a detection buffer;

[0061] The detection reaction plate includes: detection test strip, blood filter pad and absorbent paper;

[0062] The detection test strip includes: a nitrocellulose membrane and a carrier bottom plate coated with heparin-binding protein monoclonal antibody, goat anti-rabbit monoclonal antibody;

[0063] Detection buffer solution includes: 30.00g / L NaCl, 2.75g / L Na 2 HPO4, 0.75g / L KH 2 PO4, 2.5% wt PVP, 2.5% wt BSA, 0.5% wt Tween-20, 0.5% wt sodium azide, 0.5% wt proclin300 solution, 0.0010 mg / mL fluorescently labeled heparin-binding protein monoclonal antibody and 0.00010 mg / mL mL of fluorescently labeled rabbit monoclonal antibody; assay buffer pH 7.2.

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Abstract

The invention relates to the technical field of immunoassay, and especially relates to a kit for detecting a heparin binding protein by a dry-type quantitative immunofluorescence technology, and a method for detecting the heparin binding protein. The kit uses the dry-type quantitative immunofluorescence technology to in vitro quantitatively detect the heparin binding protein in human blood plasma,is a novel immunoassay kit, overcomes personal errors of an EIA technology in the technical aspects (such as skill, temperature, time and result judgment) of an operator, can provide a powerful experiment diagnosis basis for clinic diagnosis, curative effect observation and prognosis judgment by quantitatively and objectively reflecting the existence of the HBP, and is deeply widely accepted by doctors and patients. The method for detecting the heparin binding protein has the characteristics of high specificity, good repeatability, high detection sensitivity, wide linear range, high automation degree, and simplicity and rapidness in operation; and compared with the EIA method, the method in the invention has the advantages of rapidness, simplicity, accuracy, and small interference.

Description

technical field [0001] The invention relates to the technical field of immunoassays, in particular to a heparin-binding protein assay kit using an immunofluorescence dry quantitative method and a heparin-binding protein assay method. Background technique [0002] Infectious diseases are a class of diseases that seriously threaten human health. Sepsis is one of the infectious diseases with a high mortality rate. Early diagnosis and treatment play an important role in improving the efficacy and prognosis of sepsis. Therefore, the biggest clinical challenge now is how to diagnose sepsis patients as early as possible. In recent years, heparin-binding protein (HBP) has been confirmed as one of the early diagnostic markers of sepsis. [0003] Sepsis refers to a systemic inflammatory response syndrome (SIRS) caused by infection, with clinically confirmed infection or highly suspected infection. Although sepsis is caused by infection, once it occurs, its occurrence and development...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/533
CPCG01N33/533G01N33/68G01N2333/47
Inventor 王涛洪龙斌龚庆姚静岚潘慧慧陈莹菲刘文春周旭一
Owner HANGZHOU JOINSTAR BIOTECH
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