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Determination method of ellagic acid content in Cortex Moutan

A method of determination, the technology of Moutan Cortex, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple steps and easy operation

Active Publication Date: 2021-07-23
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, there is no report about containing ellagic acid in Cortex Moutan

Method used

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  • Determination method of ellagic acid content in Cortex Moutan
  • Determination method of ellagic acid content in Cortex Moutan
  • Determination method of ellagic acid content in Cortex Moutan

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Experimental program
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Effect test

Embodiment 1

[0033] 1. Instruments and reagents

[0034] Instruments: Sartorius CP225D electronic balance; Waters2695 high performance liquid chromatography.

[0035] The reference substance was provided by China National Institutes for Food and Drug Control, batch number: 111959-201602, and the content was 89.3%.

[0036] Reagents: acetonitrile and methanol are chromatographically pure, water is purified water prepared by Millipore, and other reagents are analytically pure.

[0037] 2. Chromatographic conditions

[0038] 2.1 Preparation of reference substance solution: Accurately weigh 12.09mg of ellagic acid reference substance, put it in a 100ml measuring bottle, add methanol to dissolve and dilute to the mark, shake well, use it as the reference substance stock solution, then precisely measure 1ml, put it in 25ml volume In the bottle, add methanol to dilute to the mark and shake well to obtain a reference substance solution with a concentration of 4.3 μg / ml.

[0039] 2.2 Preparation...

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Abstract

The invention relates to a method for determining the content of ellagic acid in Moutan Cortex in the technical field of traditional Chinese medicine: take Moutan Cortex medicinal material, use hydrochloric acid methanol solution to extract, filter, and take the subsequent filtrate; the subsequent filtrate is injected into a liquid chromatograph, and the detection wavelength is 253nm. The content of ellagic acid in the test solution was determined by external standard method or standard curve method. The method of the invention is easy to operate and has simple steps, and provides a certain theoretical basis for the further development and utilization of the traditional Chinese medicine Cortex Moutan.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a method for determining the content of ellagic acid in Cortex Moutan. Background technique [0002] Cortex Moutan is the dried root bark of Paeonia suffruticosa Andr., a plant in the Ranunculaceae family. The roots are excavated in autumn, fine roots and silt are removed, the root bark is stripped, dried in the sun or the rough bark is scraped off, the wood core is removed, and dried in the sun. Bitter, pungent, slightly cold, has the effects of clearing away heat and cooling blood, promoting blood circulation and removing blood stasis, clearing liver and purging fire, used for hot blood entering the camp, warming and poisoning spots, vomiting blood and epistaxis, hot night and early cooling, bone steaming without sweat, Amenorrhea dysmenorrhea, tumbling pain, carbuncle sore. [0003] Cortex Moutan has complex chemical components. Zhao Wenjun et al. used HP...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/14
CPCG01N30/02G01N30/14
Inventor 林林林永强刘广桢于凤蕊徐丽华
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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