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Calcium chelated pig bone collagen peptide and preparation method thereof

A pig bone glue and calcium chelation technology, applied in the field of bone protein processing, can solve the problems of less functional research on polypeptides, high calcium chelation of pig bone collagen peptides, etc., and achieve the effect of increasing added value

Inactive Publication Date: 2018-12-07
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on porcine bone protein is mainly focused on the optimization of protein enzymatic hydrolysis process, while there are few functional studies on polypeptides, and there is no research on high calcium chelated porcine bone collagen peptides.

Method used

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  • Calcium chelated pig bone collagen peptide and preparation method thereof
  • Calcium chelated pig bone collagen peptide and preparation method thereof
  • Calcium chelated pig bone collagen peptide and preparation method thereof

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preparation example Construction

[0020] The present invention provides a preparation method of calcium-chelating pig bone collagen peptide, which comprises the following steps: 1) taking fresh pig bones and crushing them, defatting, decalcifying, removing impurities and pulverizing to make pig bone powder; 2) making pig bone powder After being dissolved in water, mixed with alkaline protease and / or neutral protease for enzymatic hydrolysis to obtain a porcine collagen polypeptide liquid, that is, calcium-chelating porcine collagen peptide.

[0021] In the present invention, the fresh pork bones are taken and crushed to be degreasing, decalcified, protein-removed, and crushed to make pork bone meal. In the present invention, the degreasing agent for degreasing is preferably n-hexane, ether, petroleum ether or acetone, and the degreasing time is preferably 24-36 h; the decalcifying agent for decalcification is preferably citric acid, hydrochloric acid or EDTA, the decalcification time is preferably 24-48h; the ...

Embodiment 1

[0036] Pork leg bones were purchased from the farmers market of Huazhong Agricultural University. The minced meat, periosteum and bone marrow were removed, washed and crushed with a bone crusher and a small traditional Chinese medicine crusher. The bone meal is dried for later use after defatting, decalcifying and removing impurities.

[0037] Pork bone meal → add water and mix until the substrate concentration is 6% → adjust the pH value to 9.0 → add alkaline protease (the amount of enzyme added is 6000u / g) for enzymolysis in a water bath at 45°C for 5h → 100°C to kill the enzyme, cool, and centrifuge at 10000r / min 10min→ Take the supernatant to obtain the enzymatic hydrolyzate, namely calcium-chelating porcine collagen peptide.

Embodiment 2

[0039] Enzymatic hydrolysis solution in Example 1→adjust pH to 7.5, add calcium chloride (final concentration is 1g / L)→40°C for 60min reaction→9 times volume of absolute ethanol precipitation for 3h→10000r / min refrigerated centrifugation for 10min, take the precipitate → Freeze-dry to obtain calcium peptide chelate.

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Abstract

The present invention provides calcium chelated pig bone collagen peptide and a preparation method thereof and a calcium peptide chelate, and belongs to the technical field of bone protein processing.The preparation method of the calcium peptide chelate comprises the following steps: 1) pig bone powder is dissolved in water; the dissolved pig bone powder is mixed with an enzyme; the mixture is subjected to enzymatic hydrolysis to obtain a pig bone collagen polypeptide solution; and 2) pH of the pig bone collagen polypeptide solution is adjusted to be 7.2-7.8; then a calcium chloride solutionis mixed with the pig bone collagen polypeptide solution for reaction; and after ethanol precipitation reaction is conducted, a solid phase component, that is the calcium peptide chelate, is collected. The calcium chelation rate of the obtained calcium chelated pig bone collagen peptide is as high as 70.24%. A scanning electron microscopy shows that after the calcium chelated pig bone collagen peptide and calcium are chelated, the structure becomes tight granular aggregates. The calcium chelated pig bone collagen peptide opens up a new way for comprehensive utilization of pig bones and also provides a theoretical basis for a development of a novel calcium supplement preparation of polypeptide chelated calcium.

Description

technical field [0001] The invention belongs to the technical field of bone protein processing, and particularly relates to a preparation method of calcium chelated porcine collagen peptide, calcium chelated porcine collagen peptide, a preparation method of calcium peptide chelate and calcium peptide chelate. Background technique [0002] Calcium is an essential nutrient for the human body, accounting for about 1.5% to 2.2% of the total body weight. Calcium deficiency can cause diseases such as osteoporosis, hypertension, colon cancer, obesity and kidney stones. Because Chinese residents are mainly plant-based diets, calcium deficiency is particularly serious. Therefore, it is very important to find a good calcium supplement. At present, the calcium supplement preparations on the market are mainly based on inorganic calcium or organic calcium, such as calcium carbonate, calcium lactate and calcium gluconate. However, ionized calcium is prone to form calcium precipitates in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/10A23J3/34A23L33/165A23L33/18
CPCA23J1/10A23J3/341A23L33/165A23L33/18A23V2002/00A23V2200/306A23V2250/1578A23V2250/55A23V2250/204A23V2300/38A23V2300/14
Inventor 靳国锋吴文敏何立超金永国马美湖
Owner HUAZHONG AGRI UNIV
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