Multi-index detection device based on localized surface plasmon resonance sensing

A plasma resonance and localized surface technology, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as mobile medical care, remote monitoring and food hygiene source monitoring, single sensor detection index, and inconvenient integration of detection terminals. , to achieve the effect of improving test efficiency, improving detection efficiency, and high integration

Inactive Publication Date: 2018-12-04
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional sensor detection devices on the market are bulky and inconvenient to integrate in the detection terminal, making it difficult to meet the needs of mobile medical care, remote monitoring and food hygiene source monitoring
In addition, the existing sensors have single detection indicators, low detection efficiency, and insufficient detection throughput
Although high-throughput detection can be achieved by using existing biochips, there are still many problems, such as the high manufacturing cost of biochip equipment, the need for additional signal detection components in the detection process, cumbersome operation steps, and the inability to monitor samples in real time. low precision

Method used

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  • Multi-index detection device based on localized surface plasmon resonance sensing
  • Multi-index detection device based on localized surface plasmon resonance sensing
  • Multi-index detection device based on localized surface plasmon resonance sensing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] like image 3 and Figure 4 As shown, this embodiment provides a multi-index detection device based on local surface plasmon resonance sensing, including a first soft rubber layer 2, a second soft rubber layer 6, a light source 1, an ordered LSPR sensor 3, a micro The fluidic chip 4 and the signal detection element 5, the first soft rubber layer 2 is provided with a number of first installation holes, the light source 1 is installed in the first installation hole, and the first soft rubber layer 2 is loaded in order The LSPR sensor 3 and the microfluidic chip 4, the microfluidic chip 4 is covered with a second soft rubber layer 6, and the second soft rubber layer 6 is provided with a second mounting hole corresponding to the position of the first mounting hole , the signal detection element 5 is installed in the second installation hole. Wherein, the light source 1 is an LED light source or an OLED light source, preferably an LED light source; the signal detection ele...

Embodiment 2

[0044] This implementation provides a method for preparing a multi-index detection device based on localized surface plasmon resonance sensing, based on a multi-index detection device based on localized surface plasmon resonance sensing in Example 1, specifically including the following steps :

[0045] S1. Preparation of ordered LSPR sensor 3;

[0046] S2. Preparation of the microfluidic chip 4;

[0047] S3. Integration of light source 1 , ordered LSPR sensor 3 , microfluidic chip 4 and signal detection element 5 .

[0048] Wherein, the step S1 includes the following sub-steps:

[0049] S11. Preparation of a structurally uniform aluminum nanocone array master by nano-indentation technology and electrochemical etching;

[0050] S12. Pour the polydimethylsiloxane (PDMS) prepolymer into the master plate, cure at 70-90 °C for 25 min-35 min, and then peel off to obtain a PDMS nano-elastic stamp. In this embodiment, the curing temperature is preferably 80 ℃, the curing time is ...

Embodiment 3

[0065] This embodiment is similar to Embodiment 2. Further, the first soft rubber layer 2 is provided with four first installation holes, and the second soft rubber layer 6 is provided with four first installation holes corresponding to the positions of the first installation holes. Two mounting holes, that is, the obtained multi-indicator detection device can simultaneously detect four indicators of the sample, which not only increases the detection channel, but also improves the throughput of biological detection.

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Abstract

The invention relates to a multi-index detection device based on local surface plasmon resonance sensing. The multi-index detection device comprises a first soft rubber layer, a second soft rubber layer, light sources, an ordered LSPR sensor, microfluidic chips and signal detection components, wherein the first soft rubber layer is provided with a plurality of first mounting holes; the interior ofeach first mounting hole is provided with a light source; the ordered LSPR sensor and the microfluidic chips are successively loaded on the first soft rubber layer; the second soft rubber layer is laid on the microfluidic chips and provided with second mounting holes corresponding to the positions of the first mounting holes; and the interior of each second mounting hole is provided with a signaldetection component. The above-mentioned multi-index detection device provided by the invention can set a plurality of detection channels as needed to facilitate multi-index high-throughput detectionof a sample and improve testing efficiency; in addition, the above-mentioned multi-index detection device only needs to simply track testing light intensity; and once a detection marker is bind to the sample, light intensity changes, and thus, the content of the sample can be detected through detection of the light intensity.

Description

technical field [0001] The invention relates to a detection device, in particular to a multi-index simultaneous detection device based on local surface plasmon resonance sensing, which can be applied to drug research, food safety detection, disease diagnosis, cell labeling, fixed-point diagnosis and molecular interaction Identification of dynamics research and other fields. Background technique [0002] At present, in the fields of disease diagnosis, food safety and biological research, multi-index comprehensive evaluation of safety and effectiveness is used to improve the accuracy of evaluation. The use of sensors to quickly detect various indicators is one of the effective methods for evaluation. Conventional sensor detection devices on the market are bulky and inconvenient to integrate in the detection terminal, making it difficult to meet the needs of mobile medical care, remote monitoring and food hygiene source monitoring. In addition, the existing sensors have singl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/552
CPCG01N21/554
Inventor 周建华曾伟霞谭雅尹付荃莹李雪萌
Owner SUN YAT SEN UNIV
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