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Anaerobic biodegradation method for azo dye gold orange II and treatment method for wastewater

A technology of anaerobic organisms and azo dyes, which is applied in biological water/sewage treatment, anaerobic digestion treatment, water treatment parameter control, etc., and can solve problems such as increasing the cost of biological methods

Active Publication Date: 2018-12-04
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first step in the degradation of azo dyes is the breaking of the azo bond, which is easily accomplished by anaerobic microorganisms. Acetic acid, ethanol or glucose are commonly used electron donors in the anaerobic biodegradation of azo dyes, and often Additional dosing is required, which increases the cost of biological methods to a certain extent

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  • Anaerobic biodegradation method for azo dye gold orange II and treatment method for wastewater
  • Anaerobic biodegradation method for azo dye gold orange II and treatment method for wastewater

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Embodiment 1

[0046] The medium composition that this embodiment adopts is:

[0047] The composition of major elements is as follows (per L): KHCO 3 ,0.5g; KH 2 PO 4 ,0.05g; MgSO 4 ·7H 2 O, 0.2g; CaCl 2 ,0.2265g; Acidic trace elements, 0.2mL; Basic trace elements, 0.5mL. The composition of acidic trace elements is as follows (per L): FeSO 4 ·7H 2 O, 2.085g; ZnSO 4 ·7H 2 O, 0.068g; CoCl 2 ·6H 2 O, 0.12g; MnCl 2 4H 2 O, 0.5g; CuSO 4 ,0.32g; NiCl 2 ·6H 2 O, 0.095g; H 3 BO 3 , 0.014g; HCl, 100mmol. The composition of alkaline trace elements is as follows (per L): Na 2 WO 4 2H 2 O, 0.05g; Na 2 MoO 4 , 0.242g; NaOH, 0.4g.

[0048] Prepare 8g / L stock solution of the azo dye Golden Orange II: weigh 2g of the azo dye Golden Orange II and dissolve it in a beaker filled with 150mL of deionized water, then stir to dissolve, and use a 250mL volumetric flask to make it to volume. Finally, transfer to a 250mL blue bottle for later use.

[0049] According to the following method, ...

Embodiment 2

[0060] The relationship between isotope methane and concentration change of azo dye Golden Orange II

[0061] The medium composition that this embodiment adopts is:

[0062] Prepare 2g / L stock solution of azo dye Golden Orange II: Weigh 0.2g of azo dye Golden Orange II and dissolve it in a beaker filled with 50mL of deionized water, then stir to dissolve, and use a 100mL volumetric flask to volume , and finally transferred to a 100mL blue-necked bottle for later use.

[0063] Take 2 clean serum bottles with a volume of 60mL, and seal them with butyl rubber stoppers. Vacuum each for 5 minutes to ensure an anaerobic environment.

[0064] Then, take 10 mL of the inoculum slurry containing denitrifying anaerobic methane oxidizing archaea and put it into the glove box, dissolve it with 90 mL of fresh anaerobic medium and dilute it in a 150 mL beaker, mix well under a magnetic stirrer, and then transfer them separately with a 50 mL syringe Put 30mL into 2 vials (to ensure the sam...

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Abstract

The invention provides an anaerobic biodegradation method for the azo dye gold orange II. According to the anaerobic biodegradation method, a sequencing batch mode is employed; methane is used as an electron donor; azo dye gold orange II is used as an electron acceptor; and denitrification anaerobic methane oxidation (DAMO) archaea is adopted for anaerobic biodegradation of the azo dye gold orangeII. When the DAMO archaea oxidizes methane into carbon dioxide, generated electrons are transfered to the azo dye gold orange II so as to reduce the azo dye gold orange II into p-aminobenzenesulfonicacid and 1-amino-2-naphthol, and thus, the anaerobic microbial degradation of the azo dye by the DAMO archaea is realized. The anaerobic biodegradation method of the invention has great advantages ineconomical performance and environmental protection; and the DAMO archaea widely exists in nature, which is beneficial for large-scale application of the DAMO archaea in anaerobic microbial treatmentof azo dyes and even other organic substances.

Description

technical field [0001] The invention belongs to the technical field of organic wastewater treatment, and in particular relates to an anaerobic biodegradation method of azo dye Golden Orange II and a wastewater treatment method. Background technique [0002] Due to the advantages of easy synthesis, low price and good dyeing performance, azo dyes have become widely used industrial dyes in industries such as textiles, paper, leather, food, cosmetics and pharmaceuticals. Although azo dyes have the advantages of high chroma and strong stability, their molecular structure is very stable and difficult to degrade. Moreover, the degradation products of azo dyes, such as aromatic amines, can change the structure of DNA and cause lesions or even cancer. Therefore, the discharge of azo dye-containing wastewater into the environment is very harmful to organisms and human health. In addition, the discharge of wastewater containing azo dyes can also cause discoloration of the water envir...

Claims

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Application Information

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IPC IPC(8): C02F3/28C02F3/34C02F101/30
CPCC02F3/28C02F3/282C02F3/34C02F2101/308C02F2209/02C02F2209/06
Inventor 曾建雄王秀宁白亚南张伟
Owner UNIV OF SCI & TECH OF CHINA
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