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InDel molecular marker of rice blast-resistant gene Pid3-A4, and detection method and application thereof

A molecular marker, pid3-a4 technology, applied in the field of rice blast resistance gene screening, can solve the problems of high cost, low accuracy, complex molecular marker detection procedures, etc., and achieve high accuracy, good repeatability, and improved selection. effect on reproductive efficiency

Active Publication Date: 2018-11-30
HUNAN HYBRID RICE RES CENT
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Problems solved by technology

[0006] The present invention provides an InDel molecular marker, detection method and application of the rice blast gene Pid3-A4, in order to solve the problem of complex molecular marker detection procedures, low accuracy and high cost in the prior art, which is only applicable to hybrid offspring between specific parents technical issues of auxiliary detection

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  • InDel molecular marker of rice blast-resistant gene Pid3-A4, and detection method and application thereof
  • InDel molecular marker of rice blast-resistant gene Pid3-A4, and detection method and application thereof
  • InDel molecular marker of rice blast-resistant gene Pid3-A4, and detection method and application thereof

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Embodiment

[0052] (1) Functional gene sequence marker structure screening of Pid3

[0053] Download the Pid3 gene (LOC_Os06g22460) coding region from the database Rice SNP-Seek Database (http: / / snp-seek.irri.org / ) and RiceVariation Map v2.0 (http: / / ricevarmap.ncpgr.cn / v2 / ) And all the SNP and InDel information contained in the front and back 2000bp.

[0054] Such as figure 1 with figure 2 As shown, by analyzing the sequence variation of the Pid3 site in 2621 rice materials, it was found that the gene coding region of this site did not contain insertion / deletion mutations, and only a few SNP variations existed. Therefore, the target segment was extended to the sequence around the Pid3 gene coding region, and the physical location of the Pid3 gene coding sequence was 13055253-13058027 on rice chromosome 6. Through sequence comparison, it was found that there was a 9bp deletion in the Pid3-A4 functional gene at 1815 bases before the start codon at the 5' end of the Pid3 coding region co...

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Abstract

The invention discloses an InDel molecular marker of a rice blast-resistant functional gene Pid3-A4, wherein the InDel molecular marker includes an InDel molecular marker PA4-C, and the InDel molecular marker PA4-C is used for identifying and screening a rice material with a functional gene Pid3-A4 having 9 bp deletion at first 1815 bases of a coding region from rice materials; a 9 bp deletion sequence is CCCTGAAGA. The functional molecular marker can accurately detect whether a Pid3-A4 functional gene is contained in genomes of different rice varieties and detect the homozygous status, can beapplied for screening rice hybridized and transferred offspring plants, improves the breeding efficiency of the rice blast-resistant material of rice, and obtains rice blast-resistant rice varietiescontaining the functional gene Pid3-A4.

Description

technical field [0001] The invention relates to the field of rice blast resistance gene screening, in particular to an InDel molecular marker of the rice blast resistance functional gene Pid3-A4. In addition, the present invention also relates to a detection method and application of the InDel molecular marker including the rice blast resistance functional gene Pid3-A4. Background technique [0002] Rice is one of the most important food crops in the world, and more than half of the world's population uses rice as a staple food. Rice blast is the main disease that affects rice production, ranking first among fungal diseases. Rice blast causes a 10% to 30% reduction in rice production worldwide every year, and in severe cases, a 40% to 50% reduction in production, or even no harvest. Compared with chemical control of rice blast, it is economical and environmentally friendly to promote rice varieties containing disease-resistant genes. However, since a single resistance gene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/04
CPCA01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 吕启明黄志远唐丽辛业芸朱立煌
Owner HUNAN HYBRID RICE RES CENT
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