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Method of enriching short-cut denitrifying bacteria by substrate feeding and intermittent operation

A short-range denitrification, intermittent technology, applied in chemical instruments and methods, water pollutants, biological treatment devices, etc., can solve the problems of reducing the denitrification rate, reducing the nitrite reaction rate, and increasing the release amount.

Inactive Publication Date: 2018-11-27
TSINGHUA UNIV
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Problems solved by technology

DO has an inhibitory effect on denitrification, the presence of DO reduces the denitrification rate, and the micro-aerobic environment causes N 2 O production increase
In addition, the presence of high concentrations of nitrite inhibits the activity of nitrite reductase (Nir), leading to a decrease in the reaction rate of nitrite, and also inhibits the activity of nitrous oxide reductase (Nos), resulting in system N 2 O release increased

Method used

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  • Method of enriching short-cut denitrifying bacteria by substrate feeding and intermittent operation
  • Method of enriching short-cut denitrifying bacteria by substrate feeding and intermittent operation
  • Method of enriching short-cut denitrifying bacteria by substrate feeding and intermittent operation

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Embodiment

[0089] 1. Denitrification rate determination of inoculated sludge

[0090] Take sewage treatment plant A 2 / O process return sludge is inoculated sludge (the preferred sewage treatment plant A of the present invention 2 / O process reflux sludge), with a fully automatic bacterial fermentation tank as the test device, with an effective volume of 4L, such as figure 1 shown. figure 1 Among them, 1 is a display screen with buttons on it for adjusting parameters; 2 is a stirrer; 3 is a heat transfer / cooling layer; 4 is a pH electrode for monitoring the pH of the reaction solution; 5 is a temperature electrode for monitoring the temperature of the reaction solution; 6 is the DO electrode for monitoring DO in the reaction solution; 7 is the sampling port and cover; 8 is the HCl stock solution reservoir, 9 is the HCl inlet pump, used to adjust the pH and supplement the acidity; 10 is NaNO 2 and CH 3 COONa feeding liquid reserve, 11 is NaNO 2 and CH 3 COONa liquid inlet pump, used...

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Abstract

A method of enriching short-cut denitrifying bacteria by substrate feeding and intermittent operation belongs to the technical field of water treatment. Activated sludge containing short-cut denitrifying bacteria is used as inoculating sludge; a bacterial fermenting tank is utilized to allow enrichment culture of short-cut denitrifying bacterial colonies within a short term by means of substrate (nitrites and organics) feeding and intermittent operation. The short-cut denitrifying bacterial colonies attained herein are of numerical dominance in total bacteria, high nitrite-denitrifying rate can be achieved, and the capacity to carry out denitrifying by using nitrates as electron receptors is imparted.

Description

technical field [0001] The invention belongs to the field of water treatment, in particular to a method for enriching short-range denitrifying bacteria by substrate flow-intermittent operation. Background technique [0002] At present, domestic and foreign sewage treatment plants generally use activated sludge process to remove nitrogen and phosphorus. The short-cut nitrification-denitrification technology can effectively save carbon sources, aeration energy consumption, reduce sludge production and reactor footprint, and is extremely useful. It has become a research hotspot in the field of sewage treatment in the world. The concentration of functional bacteria involved in nitrogen and phosphorus removal in activated sludge of sewage treatment system is directly proportional to the reaction rate, which directly affects the effect of nitrogen and phosphorus removal in sewage treatment process. Increase the concentration of short-range denitrification bacteria to promote the ...

Claims

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Application Information

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IPC IPC(8): C02F3/30C02F101/30
CPCC02F3/301C02F3/305C02F3/307C02F3/308C02F2101/30C02F2203/004
Inventor 王凯军姚仁达
Owner TSINGHUA UNIV
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