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Oxygen-rich and temperature-sensitive hydrogel and preparation method thereof

A hydrogel, temperature-sensitive technology, used in medical science, prosthesis, tissue regeneration, etc., to achieve the effect of promoting bone defect healing, good tissue compatibility, and beneficial to bone healing

Active Publication Date: 2018-11-23
中国人民解放军联勤保障部队第九八〇医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on oxygen-enriched bone filling materials.

Method used

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  • Oxygen-rich and temperature-sensitive hydrogel and preparation method thereof
  • Oxygen-rich and temperature-sensitive hydrogel and preparation method thereof
  • Oxygen-rich and temperature-sensitive hydrogel and preparation method thereof

Examples

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preparation example Construction

[0068] The preparation method of this oxygen-enriched temperature-sensitive hydrogel is as follows: (1) Prepare chitosan (CS) acid solution: weigh CS powder and hydrochloric acid solution or The concentration of acetic acid solution, hydrochloric acid solution or acetic acid solution is 0.05~0.15mol / L; after mixing, keep stirring at room temperature at a speed of 800~1500r / min for 2~5h until the CS solution is almost clear, refrigerate overnight at 4°C or below, and then Impurities were filtered through a filter membrane with a diameter of 0.5-1.2 μm to obtain a CS solution, which was then packaged in kraft paper, sterilized in a pressure cooker at 100-150°C for 5-15 minutes, and refrigerated at or below 4°C for later use.

[0069] (2) Prepare β-sodium glycerophosphate (β-GP) solution: weigh β-GP powder and water according to the β-GP content of 10%-25% g / ml; Stir for 10 to 20 minutes until clarified, sterilize by filtration with a 0.15 to 0.22 μm filter membrane, and store in...

Embodiment 1

[0073] Embodiment 1: The preparation method of this oxygen-enriched temperature-sensitive hydrogel adopts the following specific processes.

[0074] (1) Prepare CS acid solution: weigh 0.75g of CS powder, measure 25ml of hydrochloric acid solution with a concentration of 0.1mol / L in a beaker; mix CS powder with hydrochloric acid solution, and use a magnetic stirrer at room temperature to The stirring was continued for 3 h at a speed of min until the CS solution was almost clear. Refrigerate overnight at 4°C, and then filter impurities with a 1.2 μm filter membrane to obtain a 3% g / ml CS acid solution, which is then packaged in kraft paper, sterilized in a 120°C pressure cooker for 10 minutes, and refrigerated at 4°C for later use.

[0075] (2) Preparation of β-GP solution: weigh 1.725g of β-GP powder; measure 10ml of deionized water into a beaker, add β-GP powder; stir with a magnetic stirrer at 1000r / min for 15min at room temperature Until clarification, 0.22 μm filter membr...

Embodiment 2

[0079] Embodiment 2: The preparation method of this oxygen-enriched temperature-sensitive hydrogel adopts the following specific processes.

[0080] Steps (1)-(3) are the same as in Example 1, and steps (4) and (5) are the same as in Example 1 except for the following differences, except that:

[0081] (4) Mix 95 mg egg yolk lecithin with 700 μL Tyrode salt buffer and 300 μL panflon stock solution to prepare a panfluon emulsion with a concentration of 30% g / ml.

[0082] (5) Prepare oxygen-enriched thermosensitive hydrogel: take 8ml, 3%g / ml CS solution, 2ml, 17.25%g / ml β-GP solution, 2.5ml, 3.75%g / ml HEC solution, 6. 25ml, 30%g / ml of Penflon emulsion was mixed and sealed, and fully mixed with a vibrator in an ice bath environment to prepare the oxygen-enriched hydrogel glue, which was divided into several cryopreservation tubes and stored in a sealed container at 4°C . The content of each component in each milliliter of the hydrogel in the obtained oxygen-enriched hydrogel so...

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Abstract

The invention discloses oxygen-rich and temperature-sensitive hydrogel and a preparation method thereof. The oxygen-rich and temperature-sensitive hydrogel is prepared by mixing a CS acid solution, abeta-G solution, an HEC solution and perflubron emulsion; the content of perflubron in the hydrogel is 5.0-15.0vol%. The oxygen-rich and temperature-sensitive hydrogel provided by the invention is free from toxic and side effects, has good physical and chemical properties, oxygen release performance and microstructure, is good in histocompatibility, can be completely removed by metabolism, can promote bone defect healing, and is suitable for being used in the field of bone tissue engineering as a sustained-release carrier of oxygen. The oxygen-rich and temperature-sensitive hydrogel has the basic biological characteristics of CS, is experimentally proved to have good histocompatibility and degradability, has no obvious stimulation and toxic and side effects on surrounding tissues, and canbe gradually and completely eliminated by means of metabolism; the degradation process can provide a growth space for the new bone tissues, so that bone healing is promoted.

Description

technical field [0001] The invention relates to an oxygen-enriched bone filling material, in particular to an oxygen-enriched temperature-sensitive hydrogel and a preparation method thereof. Background technique [0002] Bone injury caused by high-energy trauma has become one of the main diseases in the field of trauma. How to efficiently repair and reconstruct bone defects, nonunions and even osteonecrosis caused by such injuries has become a key topic in orthopedic research. The application of bone tissue engineering principles to repair such injuries has become a new hope in the field of bone defect repair research. After bone tissue injury, due to the interruption of blood flow or the formation of hematoma at the injury site, the local oxygen partial pressure decreases. Studies have shown that after fracture blood flow is interrupted, the partial pressure of oxygen in the central part even drops to 0% to 2%. Hypoxia can lead to abnormal bone metabolism, and hypoxia in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/20A61L27/12
CPCA61L27/12A61L27/20A61L27/52A61L2430/02C08L5/08C08L1/286
Inventor 王宇清刘国辉刘长安康强军王凌云
Owner 中国人民解放军联勤保障部队第九八〇医院
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