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Artificial dermis

A dermal and artificial technology, applied in coatings, medical science, prostheses, etc., can solve the problems of no antibacterial and antibacterial effects, achieve low cost of use, facilitate clinical application, and reduce infection rates

Inactive Publication Date: 2018-11-23
SHANGHAI CHANGHAI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention is carried out to solve the above problems. By providing an artificial dermis with a sandwich antibacterial and antibacterial layer, endowing the artificial dermis with continuous antibacterial ability, it solves the defect that the current clinical artificial dermis has no antibacterial and bacteriostatic effect.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Disinfectant Screening

[0043] 1. Experimental materials

[0044] (1) Alternative antibacterial ingredients

[0045] Including povidone iodine, chlorhexidine, bromogeramine, PHMB, compound lysozyme, and nitrofurazone. The main components, initial concentrations (that is, the concentrations routinely used in wound disinfection) and suppliers of each disinfectant are shown in Table 1.

[0046] Table 1 Information table of alternative antibacterial ingredients

[0047]

[0048] (2) Source of bacteria

[0049] The bacterial strains used in the experiment include sau, aba, and kpn. The standard strains were sau ATCC19606, abaATCC6538, and kpn CMCC(B)46117, all of which were purchased from China Common Microorganism Culture Collection Center. The clinical strains were all from Shanghai Changhai Hospital, all the strains were collected during 2015.01-2017.06, and repeated samples from the same patient at different times were removed. All strains were ident...

Embodiment 2

[0074] Embodiment 2: artificial dermis preparation

[0075] 1. Selection of artificial leather form

[0076] (1) Experimental materials

[0077] artificial leather, optional The double-layer artificial dermis repair material was provided by Shenzhen Tsinghua University Research Institute. It has a double-layer structure, a collagen layer and a silica gel layer. The collagen layer is made of bovine tendon collagen (mainly type I collagen) and has a pore size of 50-200 μm. A silicone layer and a collagen layer without adhesion were provided, respectively.

[0078] Bacteria are the aforementioned standard bacterial strains, which are made into a bacterial suspension with a concentration of 106cfu / mL, and the preparation method is the same as before.

[0079] Cell source, normal human dermal fibroblasts, supplied by PromoCell. Human vascular endothelial cells were supplied by Shanghai Bogu Biotechnology Co., Ltd.

[0080] 2. Preparation of PHMB slow-release hydrogel and gel...

Embodiment 3

[0109] Embodiment 3: Cytotoxicity and degradation experiment of artificial dermis

[0110] 1. Cytotoxicity experiment

[0111] Fibroblasts and vascular endothelial cells are one of the most important cells in the process of wound healing, so this study measured the in vitro cytotoxicity of experimental samples and artificial dermis without antibacterial ability to human dermal fibroblasts and human vascular endothelial cells. The target cells were subcultured in cell culture medium supplemented with 10% fetal bovine serum. The experimental samples and the artificial dermis without antibacterial ability were made into circles with a diameter of 16 mm, and placed in 24-well plates respectively. The artificial dermis without antibacterial ability was added as a positive control group, and a blank control group was set up in addition. For other treatments, add 500 μL containing 1×10 6 cell / L cell culture medium. 35°C, 5% CO 2 After incubation for 3, 7, 14, and 21 days, the num...

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Abstract

The invention relates to the technical field of biological wound treatment products and provides artificial dermis with the effects of bacteria resistance and bacteriostasis. The artificial dermis isof a three-layer structure and is sequentially provided with a silica gel layer, a bacteria resistance and bacteriostasis layer and a collagen layer from top to bottom, wherein the bacteria resistanceand bacteriostasis layer is a PHMB hydrogel layer with the thickness being 1-1.5mm; and as PHMB has relatively strong bacteria resistance ability, and a clinical strain is relatively high in sensibility to the PHMB and is a proper bacteria resistance component endowing the artificial dermis with continuous bacteria resistance. Verified through experiments, after 1 percent PHMB hydrogel layer is added between the collagen layer and the silica gel layer of the artificial dermis, the biological toxicity of an experimental sample is relatively low, and the structure of the collagen layer of the artificial dermis is not obviously influenced, so that after the experimental sample of the artificial dermis with the bacteria resistance ability is transplanted to a clean wound, influence on wound healing is relatively small, bacteria can be effectively resisted, and the infection rate is lowered; and after transplanting, although infection factors continuously exist, the effect of preventing infection can be exerted within a certain time.

Description

technical field [0001] The invention relates to the technical field of biological trauma treatment products, in particular to an artificial dermis with antibacterial and antibacterial effects. Background technique [0002] As a substitute for autologous skin, artificial dermis can be used for wounds with damaged dermis, chronic wounds or burn wounds. It has the functions of promoting wound healing and reducing scar formation, and overcomes the shortage of autologous skin resources in patients with extensive burns. , so favored by clinicians. However, since the artificial dermis failed to establish a reliable blood supply with the wound in the early stage after transplantation, the antibacterial ability was poor, and the infection rate after transplantation was high. Once infection occurred, it would not only lead to graft failure, but even threaten the life of the patient. [0003] At present, the commonly used clinical measures to prevent infection after artificial skin tr...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/60A61L27/54A61L27/02A61L27/24A61L27/56
CPCA61L27/025A61L27/24A61L27/54A61L27/56A61L27/60A61L2300/206A61L2300/404A61L2300/606
Inventor 王光毅金剑汤涛周浩相阳何放徐龙夏照帆
Owner SHANGHAI CHANGHAI HOSPITAL
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