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Electrochemical biosensor for detecting ampicillin and preparation method thereof

A biosensor, ampicillin technology, which is applied in the field of electrochemical analysis, can solve the problems of weak detection specificity, expensive operation of the instrument, and high detection cost, and achieves the effects of realizing miniaturization of the instrument, improving detection sensitivity, and simple detection method.

Inactive Publication Date: 2018-11-20
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have the disadvantages of low detection sensitivity, low detection specificity, high detection cost, expensive instruments, complicated operation, and the need for professional operators. Therefore, it is necessary to establish a simple, fast, high sensitivity and high selectivity method. , it is very necessary and urgent to realize the detection of ampicillin in food

Method used

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  • Electrochemical biosensor for detecting ampicillin and preparation method thereof
  • Electrochemical biosensor for detecting ampicillin and preparation method thereof
  • Electrochemical biosensor for detecting ampicillin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1: glassy carbon electrode pretreatment

[0063] The glassy carbon electrode was polished with 30nm aluminum oxide slurry, and then polished with 200 mesh aluminum oxide slurry. Then, the glassy carbon electrode was ultrasonically cleaned in deionized water and ethanol for 15 min, respectively. Blow dry with nitrogen.

Embodiment 2

[0064] Embodiment 2: the modification of nano gold

[0065] Immerse the pretreated glassy carbon electrode in 10 mL of 3 mM HAuCl 4 and 0.1M KNO 3 In the mixed solution, use the constant potential deposition technique (potential is -0.2V), and electrodeposit for 200 seconds under magnetic stirring. Then, the electrodes were washed three times with a washing solution. Blow dry with nitrogen. The prepared electrode is labeled as AuNPs / GCE.

Embodiment 3

[0066] Embodiment 3: the modification of probe DNA

[0067] Add 10 μL of probe immobilization buffer (10 mM Tris-HCl, 1.0 mM EDTA, 1.0 M NaCl, 1.0 mM TCEP, pH 7.4) containing 1.0 μM probe DNA dropwise onto the surface of the AuNPs / GCE electrode at room temperature and under humid conditions After reacting for 12 hours, wash with washing solution 3 times. The electrodes are labeled: ssDNA / AuNPs / GCE.

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Abstract

The invention discloses an electrochemical biosensor for detecting ampicillin and a preparation method thereof. The electrochemical biosensor includes an electrode, and nanogold, a probe DNA and an aptamer DNA that are sequentially modified on the surface of the electrode. A sequence of the probe DNA is shown as SEQ ID NO.1; and the sequence of the aptamer DNA is shown as SEQ ID NO.2. The electrochemical biosensor utilizes good biocompatibility and conductivity of the nanogold, so that amplification of a photoelectric signal is achieved, and the detection sensitivity of the ampicillin is improved. A detection method of the electrochemical biosensor is simple, an instrument is miniaturized, and an operation is easy; and the ampicillin can be detected only by simply processing the surface ofa glassy carbon electrode.

Description

technical field [0001] The invention relates to the technical field of electrochemical analysis, in particular to an electrochemical biosensor for detecting ampicillin and a preparation method thereof. Background technique [0002] Ampicillin is a semi-synthetic broad-spectrum penicillin. The antibacterial mechanism is to prevent the synthesis of bacterial cell walls, so it can not only inhibit its proliferation, but also directly kill bacteria. The effect on Gram-positive bacteria is similar to that of penicillin, the effect on Streptococcus viridans and Enterococcus is better, the effect on other bacteria is poor, and it is invalid against penicillin-resistant Staphylococcus aureus. Among Gram-negative bacteria, Neisseria gonorrhoeae, meningococcus, influenza bacillus, pertussis bacillus, Escherichia coli, typhoid and paratyphoid bacillus, Shigella, Proteus mirabilis, Brucella, etc. are sensitive to this product, but are prone to drug resistance sex. Klebsiella pneumonia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/3277G01N27/3278G01N27/48
Inventor 殷焕顺周云雷艾仕云
Owner SHANDONG AGRICULTURAL UNIVERSITY
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