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Method for detecting blending of dairy cow milk into buffalo milk and buffalo milk dairy products by duplex polymerase chain reaction

A technology of chain reaction and detection method is applied in the field of double polymerase chain reaction detection of buffalo milk and dairy products incorporating dairy milk sources, which can solve the problem of inability to detect dairy products, reduced sensitivity of milk and dairy products, etc. problem with low cost and good repeatability

Inactive Publication Date: 2012-12-05
GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional immunological methods and electrophoresis techniques mainly identify proteins, so the sensitivity of these two methods is greatly reduced in heat-treated milk and dairy products, and they cannot be used for dairy products that have added a variety of unknown exogenous protein additives. carry out testing

Method used

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  • Method for detecting blending of dairy cow milk into buffalo milk and buffalo milk dairy products by duplex polymerase chain reaction
  • Method for detecting blending of dairy cow milk into buffalo milk and buffalo milk dairy products by duplex polymerase chain reaction
  • Method for detecting blending of dairy cow milk into buffalo milk and buffalo milk dairy products by duplex polymerase chain reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Detection of fresh buffalo milk samples mixed with different proportions of cow milk

[0040] Such as figure 1As shown, buffalo-specific genes were detected in fresh buffalo milk spiked with 0%, 0.5%, 5%, 20 and 50% cow milk (lanes 1-5), but not in the 100% spiked group ; Cow-specific genes were detected in fresh buffalo milk spiked with 0.5%, 5%, 20%, 50% and 100% of cow's milk, but not detected in buffalo milk spiked with 0% (Tracks 2-6), indicating that the PCR method can detect 0.5% cow milk incorporation in fresh buffalo milk.

Embodiment 2

[0042] Detection of buffalo milk mozzarella cheese samples mixed with different proportions of cow milk

[0043] Such as figure 2 As shown, buffalo-specific genes were detected in cheeses spiked with 0%, 0.5%, 5%, 20, and 50% cow’s milk (lanes 1-5), but not in the 100% spiked group; Specific genes were detected in buffalo milk mozzarella cheese mixed with 0.5%, 5%, 20%, 50% and 100% cow milk, but not in buffalo milk mixed with 0% ( Tracks 2-6), showing that the PCR method can detect 0.5% cow milk incorporation in buffalo milk mozzarella cheese.

Embodiment 3

[0045] Detection of buffalo milk yogurt samples mixed with different proportions of cow milk

[0046] Depend on image 3 As can be seen, buffalo-specific genes were detected in cheeses spiked with 0%, 0.5%, 5%, 20, and 50% cow's milk (lanes 1-5), but not in the 100% spiked group; cow-specific Sex genes were detected in buffalo milk yoghurt mixed with 0.5%, 5%, 20%, 50% and 100% cow milk, but not in buffalo milk mixed with 0% milk (No. 2 -6 track), indicating that the PCR method can detect 0.5% cow milk incorporation in buffalo milk yoghurt.

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Abstract

A method for detecting blending of dairy cow milk into buffalo milk and buffalo milk dairy products by duplex polymerase chain reaction includes the steps of PCR (polymerase chain reaction) amplification of buffalo milk genome DNA (deoxyribonucleic acid) samples to be detected and electrophoretic analysis on PCR products, three primers C1, B1 and B2 are adopted for forming two pairs of PCR reaction, and the primer design scheme of a PCR system includes: the primer C1 and the primer B1 form a pair of primers for amplification of buffalo specificity gene segments, and amplification products are the buffalo specificity gene segments; and the primer C1 and the primer B2 form a pair of primers for amplification of dairy cow specificity gene segments, and amplification products are the dairy cow specificity gene segments. The method has the advantages of rapidness, accuracy and sensitivity and can complete detection by one-time PCR amplification.

Description

technical field [0001] The invention relates to a PCR method for detecting buffalo milk and its dairy products mixed with common cow milk. Background technique [0002] At present, the quality of buffalo milk and its dairy products sold in my country’s market is uneven, and there are many phenomena of passing off ordinary milk as buffalo milk. For example, it was recently revealed that a quarter of the Italian famous brand mozzarella buffalo cheese is not pure buffalo milk. Some domestic buffalo milk production companies were exposed that the sales volume of buffalo milk products did not match the actual raw milk production. All of the above have affected the stable development of my country's buffalo milk market. Italy and other countries have established standard detection methods for buffalo milk and its dairy products mixed with ordinary cow milk, which plays an important role in protecting their characteristic buffalo milk products. The reported detection techniques for...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 林波曾庆坤杨炳壮李玲唐艳农皓如
Owner GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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