Water-soluble fluorescent silicon point as well as preparation method and application thereof

A water-soluble and fluorescent technology, applied in the field of analysis and detection, can solve the problems of distinguishing and quantifying flavonoids, difficult to distinguish and quantify, and achieve the effects of overcoming poor sensitivity, simple preparation method and stable performance.

Active Publication Date: 2018-11-20
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

However, flavonoids are very similar in spectral behavior. Generally, it is difficult to distinguish and quantify rutin from other flavonoids by spectral methods. In particular, there is only one glycoside difference between quercetin and rutin in structure. The properties are very close, and it is very difficult to distinguish and quantify the two with conventional spectral methods

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  • Water-soluble fluorescent silicon point as well as preparation method and application thereof
  • Water-soluble fluorescent silicon point as well as preparation method and application thereof
  • Water-soluble fluorescent silicon point as well as preparation method and application thereof

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preparation example Construction

[0026] The preparation method of the water-soluble fluorescent silicon dots comprises dissolving ferulic acid and (3-aminopropyl)trimethoxysilane (APTMS) in high-purity water, mixing them thoroughly and reacting at 150-180°C for 60-180min, After the reaction is completed, the mixture solution is obtained by cooling, and the obtained mixture solution is dialyzed to obtain water-soluble fluorescent silicon dots.

[0027] In the synthesis of nanoparticles based on the hydrothermal method, commonly used reducing agents include sodium borohydride, ascorbic acid, and sodium citrate. And the used ferulic acid another name of the present invention is 3-methoxy-4-hydroxycinnamic acid, is a kind of phenolic acid widely present in plants, has powerful antioxidant activity, and antiradiation, antioxidation and antibacterial antiviral and many other pharmacological effects, and low toxicity, so it has a wide range of uses in medicine, health care products, cosmetic raw materials and food a...

Embodiment 1

[0046] Embodiment one (best synthesis condition):

[0047] A kind of water-soluble fluorescent silicon point, its preparation method comprises the following steps:

[0048] (1) 0.8g of ferulic acid and 5mLAPTMS were mixed and dissolved in 10mL of high-purity water, and evenly stirred in a magnetic stirrer for 10min to obtain an initial mixed solution;

[0049] (2) Transfer the initial mixed solution obtained in step (1) into a polytetrafluoroethylene autoclave, heat up to 180° C. and react for 90 minutes, and cool down after the reaction to obtain a colorless and transparent mixture solution;

[0050] (3) Transfer the mixture solution obtained in step (2) to a 1KDa dialysis bag, wash with ultrapure water for 48 hours to remove excess precursors and impurities, and obtain the water-soluble fluorescent silicon dots.

Embodiment 2

[0052] A kind of water-soluble fluorescent silicon point, its preparation method comprises the following steps:

[0053] (1) 0.5g of ferulic acid and 5mLAPTMS were mixed and dissolved in 10mL of high-purity water, and evenly stirred in a magnetic stirrer for 10min to obtain an initial mixed solution;

[0054] (2) Transfer the primary mixed solution obtained in step (1) into a polytetrafluoroethylene autoclave, heat up to 150° C. and react for 60 minutes, then cool to obtain a colorless and transparent mixture solution;

[0055] (3) Transfer the mixture solution obtained in step (2) to a 1KDa dialysis bag, wash with ultrapure water for 48 hours to remove excess precursors and impurities, and obtain the water-soluble fluorescent silicon dots.

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Abstract

The invention relates to a water-soluble fluorescent silicon point as well as a preparation method and an application thereof. The water-soluble fluorescent silicon point is synthesized from (3-aminopropyl) trimethoxy silane as a precursor and ferulic acid as a reducer by a hydrothermal reaction. The fluorescent silicon point can be applied to detection of rutin content and identification of rutinand quercetin. The fluorescent silicon point has the advantages of being stable in performance, green and environmentally friendly, and the preparation method is simple. The fluorescent silicon pointas a spectral signal probe can realize rutin detection in a serum system or a phosphate buffer system containing a small amount of BSA, and other common traditional Chinese medicine active substancesincluding quercetin have no obvious interference effect on a detection result. The detection method has the advantages of being fast, simple, good in detection sensitivity, high in selectivity and the like, defects of poor sensitivity, high interference, complicated pretreatment or expensive instruments of a conventional detection method are overcome, and the detection method is a simple and practical pharmaceutical analysis and quantification technology.

Description

technical field [0001] The invention relates to the field of analysis and detection, in particular to a water-soluble fluorescent silicon dot and its preparation method and application. Background technique [0002] As a representative zero-dimensional silicon nanomaterial, fluorescent silicon dots have great prospects in life analysis and food and drug detection applications due to their good fluorescence stability, excellent biocompatibility and low toxicity. However, fluorescent silicon dots usually have poor water dispersibility due to the surface-covered hydrophobic functional groups. Some methods for synthesizing water-soluble fluorescent silicon dots (SiNCs) have been reported in recent years, but these methods usually require at least two independent steps: first, large-scale Si-based materials are used as Si sources (e.g., crystalline Si particles, SiOx powder or SiNCs). nanowires, etc.) to prepare hydrophobic silicon dots; after that, the hydrophobic silicon dots ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 许惠凤张诗琪朱希余丽双王丽丽叶蕻芝
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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