Preparation method and application of fluorescent platinum nanoclusters for optical imaging of suspended cells in blood system
A suspension cell and blood system technology, applied in the field of new materials, can solve the problems of difficult calibration and detection of suspension cells, and achieve the effects of easy operation, small size and simple preparation method
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Embodiment 1
[0017]Preparation method of fluorescent platinum nanoclusters: add H in 3.0 mL water2PtCl6And polyvinyl imine PEI, finally H2PtCl6The concentrations of and PEI were 0.1 mmol / L and 2 mmol / L, respectively. After mixing thoroughly at 25 ℃ for 30 min, add 0.1 mmol L-ascorbic acid, heat the solution to 90 ℃, react for 2 h and then cool naturally to obtain a light yellow solution. After ultracentrifugation of the crude product and dialysis with a dialysis bag for 1 d, the final fluorescent platinum nanoclusters were obtained, and the clusters emitted light blue fluorescence under ultraviolet light irradiation.
[0018]Fluorescence imaging of suspended cells in the blood system: Take the fluorescent platinum nanoclusters finally obtained in the above step and add them to sterile RPMI-1640 culture medium (containing 10% serum) containing K562 cells. The final fluorescent platinum nanocluster concentration is 0.001mmol / L. Pure air (5% CO2), after co-cultivation for 4 h, fix the suspended cells ...
Embodiment 2
[0020]Preparation method of fluorescent platinum nanoclusters: add H in 3.0 mL water2PtCl6And polyvinyl imine PEI, finally H2PtCl6The and PEI concentrations were 0.6 mmol / L and 2 mmol / L, respectively. After mixing well at -4 ℃ for 24 h, add 0.1 mmolL-ascorbic acid, heat the solution to 90 ℃, react for 4 days and then cool naturally to obtain a yellow solution. After ultracentrifugation of the above crude product and dialysis with a dialysis bag for 2 days, the final fluorescent platinum nanoclusters were obtained, and the clusters emitted yellow fluorescence under ultraviolet light irradiation.
[0021]Fluorescence imaging of suspended cells in the blood system: Take the fluorescent platinum nanoclusters finally obtained in the above step and add them to sterile RPMI-1640 culture medium (containing 10% serum) containing K562 cells. The final fluorescent platinum nanocluster concentration is 0.001mmol / L. Pure air (5% CO2), after co-cultivation for 4 h, fix the suspended cells with 4% pa...
Embodiment 3
[0023]Preparation method of fluorescent platinum nanoclusters: add H in 3.0 mL water2PtCl6And polyvinyl imine PEI, finally H2PtCl6The and PEI concentrations were 0.6 mmol / L and 2 mmol / L, respectively. After fully mixing for 1.5 h at 60 ℃, add 0.1 mmol L-ascorbic acid, heat the solution to 90 ℃, react for 4 days and then cool naturally to obtain a yellow solution. After ultracentrifugation of the above crude product and dialysis with a dialysis bag for 2 days, the final fluorescent platinum nanoclusters were obtained, and the clusters emitted yellow fluorescence under ultraviolet light irradiation.
[0024]Fluorescence imaging of suspended cells in the blood system: Take the final fluorescent platinum nanocluster obtained in the above step and add it to sterile RPMI-1640 culture medium (containing 10% serum) containing BV173 cells. The final fluorescent platinum nanocluster concentration is 0.005 mmol / L. Pure air (5% CO2), after co-cultivation for 4 h, fix the suspended cells with 4% pa...
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