Influence of WS6 on actin formation of trabecular meshwork cells

A technology of actin and trabecular meshwork, applied in the medical field, can solve problems such as high intraocular pressure and achieve the effect of reducing intraocular pressure

Active Publication Date: 2018-11-20
QINGDAO UNIV
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Problems solved by technology

The resistance to aqueous humor efflux mainly comes from the trabecular meshwork efflux pathway, and the aqueous humor efflux pathway is usually abnormal in patients with primary open-angle glaucoma, resulting in high intraocular pressure

Method used

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  • Influence of WS6 on actin formation of trabecular meshwork cells
  • Influence of WS6 on actin formation of trabecular meshwork cells

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Experimental program
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Embodiment Construction

[0013] The present invention will be described in detail below in combination with specific embodiments.

[0014] Cytotoxicity of 1WS6 to trabecular meshwork cells

[0015] First, we selected WS6 at four gradient concentrations of 10μM, 100μM, 200μM, and 300μM to test its toxicity to trabecular meshwork cells. The results showed that after 24 hours, with the increase of WS6 concentration, the death rate of trabecular meshwork cells gradually increased, and the death rate reached about 50% at 200 μM. Therefore, we first selected 10μM WS6 for pharmacodynamic experiments.

[0016] 2WS6 inhibits actin formation in trabecular meshwork cells

[0017] We studied the inhibitory effect of WS6 on actin formation in trabecular meshwork cells by phalloidin specific fluorescent staining technique and laser confocal technique. Such as figure 1 , figure 2 The results shown showed that the TRPV4 agonist GSK101 could significantly enhance the fluorescence intensity of actin in trabecular...

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Abstract

The invention discloses influence of WS6 on actin formation of trabecular meshwork cells. WS6 refers to anti-diabetic medicine glibenclamide, sodium orthovanadate and an active compound capable of promoting pancreatic beta cell proliferation. WS6 has cytotoxicity on the trabecular meshwork cells and inhibits actin formation of the trabecular meshwork cells. The influence of WS6 has the beneficialeffect that the fact that WS6 can remarkably inhibit actin formation, caused by TRPV4 agonist GSK101, of the trabecular meshwork cells is revealed. Intraocular pressure can be reduced, and the influence of WS6 has potential treatment significance in glaucoma caused by increase of intraocular pressure.

Description

technical field [0001] The invention belongs to the field of medical technology, and relates to the influence of WS6 (insulin secretion-stimulating drug glibenclamide, sodium orthovanadate and islet cell proliferation-promoting drug) on ​​actin formation of trabecular meshwork cells. Background technique [0002] Glaucoma (Glaucomas) is a disease of the optic nerve caused by the degeneration of retinal ganglion cells, which can lead to sunken optic discs, visual field defects, and eventually blindness. [0003] Studies have shown that high intraocular pressure is an important therapeutic factor for glaucoma. Every 1mm Hg reduction in intraocular pressure can reduce the progression of glaucoma by about 10%. High intraocular pressure is mainly caused by an imbalance in the aqueous humor circulation in the anterior segment. Aqueous humor is produced in the ciliary body, enters the aqueous humor circulation in the anterior segment through the narrow gap between the iris and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/64A61K33/00A61K45/00A61P27/06G01N21/64
CPCA61K31/64A61K33/00A61K45/00A61P27/06G01N21/6486
Inventor 张从晓王克威朱玮于红霞
Owner QINGDAO UNIV
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