Application of sophora tonkinensis gapnep lupanol

A technology of stigmastanol and kale, which is applied in the field of medical application of the kale root compound lupinol, can solve the problems of increasing cancer burden and shortage of drugs for nasopharyngeal carcinoma, achieving significant effects, inhibiting metastasis, and good application foreground effect

Inactive Publication Date: 2018-11-20
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the future, as my country's population aging continues to increase, the incidence and mortality of nasopharyngeal carcinoma may continue to rise, and the resulting cancer burden will continue to increase, and the prevention and control situation is severe. Currently, the effective treatment of nasopharyngeal carcinoma medicines are in short supply

Method used

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  • Application of sophora tonkinensis gapnep lupanol
  • Application of sophora tonkinensis gapnep lupanol
  • Application of sophora tonkinensis gapnep lupanol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation method of bean root lupine alkanol, it may further comprise the steps:

[0028] Step 1, in parts by weight, place 55 parts by weight of the kale root powder in 75% ethanol with a volume percentage of 5 times the weight of the yaman root powder, soak it in 75% ethanol, filter after 7 days, collect the filtrate, repeatedly soak and filter Three times, the three filtrates were combined and concentrated under reduced pressure to recover ethanol to obtain the extract;

[0029] Step 2, acidifying the extract with concentrated hydrochloric acid to pH = 3-4, then using ethyl acetate as a solvent for extraction, collecting the upper layer solution and recovering the ethyl acetate under pressure to obtain a crude extract;

[0030] Step 3. After mixing the crude extract with 1 times the weight of silica gel, add the upper part of the silica gel column. The weight of the silica gel used in the silica gel column is 10 times the weight of the crude extract after mixin...

Embodiment 2

[0043] Such as figure 1 As shown, through the MTT experiment, it is verified that lupine alcohol has a particularly obvious growth inhibitory effect on nasopharyngeal carcinoma cells:

[0044] Experimental materials: MTT and DMSO were purchased from Sigma, USA.

[0045] experimental method:

[0046] Blank control group: 100 μL with a density of 2×10 4 cells / ml of cell suspension and 100 μL of RPMI-1640 medium.

[0047] Negative control group: 100 μL at a density of 2 x 10 4 cells / ml of cell suspension and 100 μL of RPMI-1640 medium containing 0.1% DMSO.

[0048] Experimental group: 100 μL with a density of 2 x 10 4 cells / ml of cell suspension and 100 μL of lupeol at a concentration of 50 μg / ml were added.

[0049] Nasopharyngeal carcinoma cells CNE-2 in the logarithmic growth phase were taken to make a concentration of 2×10 4 cells / ml single cell suspension and inoculated in a 96-well plate, add 100 μL of cell suspension to each well, and place the 96-well plate at 37°C...

Embodiment 3

[0058] Lupine alcohols have a particularly pronounced growth-inhibitory effect on esophageal cancer cells:

[0059] Experimental materials: MTT and DMSO were purchased from Sigma, USA.

[0060] experimental method:

[0061] Blank control group: 100 μL with a density of 2×10 4 cells / ml of cell suspension and 100 μL of RPMI-1640 medium.

[0062] Negative control group: 100 μL at a density of 2 x 10 4 cells / ml of cell suspension and 100 μL of RPMI-1640 medium containing 0.1% DMSO.

[0063] Experimental group: 100 μL with a density of 2 x 10 4 cells / ml of cell suspension and 100 μL of lupeol at a concentration of 50 μg / ml were added.

[0064] Esophageal cancer cells Eca-109 in the logarithmic growth phase were taken to make a concentration of 2×10 4 cells / ml single cell suspension and inoculated in a 96-well plate, add 100 μL of cell suspension to each well, and place the 96-well plate at 37°C, 5% CO 2 After 12 hours, add 100 μL of drugs with a concentration of 50 μg / ml to ...

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Abstract

The invention discloses application of sophora tonkinensis gapnep lupanol, and particularly discloses application of the sophora tonkinensis gapnep lupanol to preparation of medicinal preparations fortreating tumors. The sophora tonkinensis gapnep lupanol provided by the invention has very good antitumor activity, can significantly inhibit growth of nasopharyngeal cancer cells, cervical cancer cells, esophageal cancer cells, liver cancer cells, laryngeal cancer cells, breast cancer cells and prostate cancer cells and inhibit metastasis of the nasopharyngeal cancer cells, and has a good application prospect in development into medicines for treating nasopharyngeal cancer and other diseases.

Description

technical field [0001] The invention relates to the field of medicine, food and health care products, in particular to the medical use of a bean root compound lupine alkanol. Background technique [0002] Tumor refers to the new organism formed by the proliferation of local tissue cells under the action of various tumorigenic factors. With the improvement of people's material living standards, people are exposed to more and more types of food and beverages in their daily lives. While people are enjoying it, they also increase the probability of suffering from tumors. Big. [0003] Nasopharyngeal carcinoma has become the main cancer and cause of death in malignant tumors of the ear, nose, and throat. The incidence rate in my country decreases from south to north, especially in Guangdong and Guangxi. The incidence rate increases with age in people over 20 years old and reaches a peak at around 45 to 60 years old. The etiology of nasopharyngeal carcinoma may be related to fa...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61P35/00A61P3/06A61P3/04A61P3/10A61P21/04
CPCA61K31/56A61P3/04A61P3/06A61P3/10A61P21/04A61P35/00
Inventor 缪剑华姚彩云宋志军王硕龚小妹
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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