Kit and detection method for quickly detecting aluminium phosphide in edible mushroom and cereals

A technology of aluminum phosphide and edible fungi, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve problems such as toxic and harmful, easy to pollute the environment, and complicated operation process , to achieve the effect of easy judgment, cheap and easy to obtain reagents, and simple and convenient use

Inactive Publication Date: 2018-11-16
郑州华洲集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of most of the reagents in the existing aluminum phosphide determination method, which are poisonous and harmful, easy to pollute the environment, and the operation process is complic...

Method used

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  • Kit and detection method for quickly detecting aluminium phosphide in edible mushroom and cereals
  • Kit and detection method for quickly detecting aluminium phosphide in edible mushroom and cereals
  • Kit and detection method for quickly detecting aluminium phosphide in edible mushroom and cereals

Examples

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Embodiment 1

[0020] A kit for rapidly detecting aluminum phosphide in edible fungi and grains, the kit includes a bottle of detection solution A, a bottle of detection solution B, a 1.5ml centrifuge tube and a 40mL sample cup;

[0021] The concentration in the detection solution A bottle is 1mol / L sodium acetate-acetic acid buffer solution, the solvent is distilled water, and the pH value of the solution is 5.0;

[0022] The test solution B bottle is filled with catechol violet with a concentration of 1.5g / L, and the solvent is distilled water.

[0023] The specific steps of using the kit for detection are as follows:

[0024] Step 01) Cut the dried fungus sample into pieces, weigh 1g into a sample cup, add 10mL of distilled water, soak for 5-10min, oscillate several times during the period, and take the supernatant as the test solution after standing still.

[0025] Step 02) Set up the control group and the experimental group, in which the experimental group: take 0.5mL of the test solut...

Embodiment 2

[0032] A kit for rapidly detecting aluminum phosphide in edible fungi and grains, the kit includes a bottle of detection solution A, a bottle of detection solution B, a 1.5ml centrifuge tube and a 40mL sample cup;

[0033] The concentration in the detection solution A bottle is 0.5mol / L sodium acetate-acetic acid buffer solution, the solvent is distilled water, and the pH value of the solution is 5.0;

[0034] The test solution B bottle is filled with catechol violet with a concentration of 0.8g / L, and the solvent is distilled water.

[0035] The specific steps of using the kit for detection are as follows:

[0036] Step 01) Take a grain (dried wheat) sample and cut it into pieces, weigh 1g into a sample cup, add 10mL of distilled water, soak for 5-10min, oscillate several times during the period, and take the supernatant as the test solution after standing still.

[0037]Step 02) Set up the control group and the experimental group, where the experimental group: take 0.5mL of...

Embodiment 3

[0044] A kit for rapidly detecting aluminum phosphide in edible fungi and grains, the kit includes a bottle of detection solution A, a bottle of detection solution B, a 1.5ml centrifuge tube and a 40mL sample cup;

[0045] The concentration in the test solution A bottle is 2mol / L sodium acetate-acetic acid buffer solution, the solvent is distilled water, and the pH value of the solution is 5.0;

[0046] The test solution B bottle is equipped with catechol violet with a concentration of 3g / L, and the solvent is distilled water.

[0047] The specific steps of using the kit for detection are as follows:

[0048] Step 01) Take an appropriate amount of dried shiitake mushrooms and chop them into pieces, weigh 1g into a sample cup, add 10mL of distilled water, soak for 5-10min, shake several times during the period, and take the supernatant as the test solution after standing still.

[0049] Step 02) Set up the control group and the experimental group, in which the experimental gro...

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Abstract

The invention discloses a kit and a detection method for quickly detecting aluminium phosphide in edible mushroom and cereals. The kit comprises: a detection solution A bottle filled with a 0.2-4 mol/L sodium acetate-acetic acid buffer solution, a detection solution B bottle filled with a 0.2-5 g/L pyrocatechol violet liquid, a centrifugal tube, and a sample cup. The detection method includes thesteps of: extracting the aluminium phosphide with water to decompose the aluminium phosphide into hydrogen phosphide and Al(OH)3, wherein the Al(OH)3 can form a violet complex with the pyrocatechol violet when pH is 5.0, and according to different concentrations, the violet complex shows a red color, a purple color or a violet color in a solution; and determining whether the sample contains the aluminium phosphide or not by observing the color of the solution after the reaction. The kit for quickly detecting aluminium phosphide in edible mushroom and cereals employs low-cost and accessible consumable reagents, is free of environmental pollution, is easy and convenient to use, and has high sensitivity and intuitive results.

Description

technical field [0001] The invention relates to the field of food safety, in particular to a kit and a detection method for rapidly detecting aluminum phosphide in edible fungi and grains. Background technique [0002] Aluminum phosphide is a phosphide pesticide, mainly used in granary storage, tobacco leaf storage, and epidemic prevention departments. Aluminum phosphide decomposes with water to produce phosphine, a highly toxic gas. When the air contains 0.1mg / L phosphine, it has a lethal effect on pests. After the aluminum phosphide in the food enters the human body, it acts on cell enzymes, affects cell metabolism, and in severe cases, internal suffocation will occur. In addition, it also stimulates and corrodes the gastrointestinal tract, and causes damage to the central nervous system, cardiovascular system, respiratory system, liver and kidney. In order to prevent the fungus from being damaged by pests, aluminum phosphide is routinely used for fumigation to remove pe...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 何招华何贞龙郭水超申战宾陈军丽张欣赵晋晋
Owner 郑州华洲集团股份有限公司
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