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Preparation method of solid probiotic preparation and solid probiotic preparation

A probiotic preparation and probiotic technology, applied in the field of preparation of solid probiotic preparations, can solve problems such as high cell death rate, short processing time, and hidden safety hazards in finished products

Active Publication Date: 2018-11-16
广州富诺营养科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the shortcomings of high cell death rate and hidden safety hazards in the finished product in the freeze-drying process in the prior art, the invention provides a preparation method of a solid probiotic preparation and its preparation, which includes adding betaine to the probiotic suspension and hydroxyproline, and improve the antifreeze ability of probiotics by gradually lowering the temperature, and then add glucose and sorbitol to it and freeze-dry it. % or more, it is significantly better than the survival rate of probiotics obtained according to the prior art of probiotic solid preparations, the steps are simple, the processing time is short, and it is suitable for popularization and application in the existing process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method of a solid probiotic preparation, which specifically comprises the following steps:

[0023] 1) centrifuging the probiotic fermentation broth at a centrifugation speed of 8000 rpm and a centrifugation time of 3 minutes to obtain probiotic cells, suspending the probiotic cells in 0.9% normal saline to obtain a probiotic cell suspension;

[0024] 2) Add betaine and hydroxyproline to the probiotic cell suspension at room temperature so that the final concentration of betaine is 0.1%, the final concentration of hydroxyproline is 0.06%, and the temperature of the control cell suspension is 0 ℃ for 2 hours, adjust the temperature to -15 ℃ for 40 minutes; adjust the temperature to -30 ℃ for 4 hours; restore the temperature to room temperature;

[0025] 3) Add 4% by weight sorbitol and 4% by weight glucose to the probiotic cell suspension, put it into a vacuum freeze dryer and freeze-dry, and control the freeze-drying temperature to -25°C-- 35°C, freeze-dr...

Embodiment 2

[0028] A preparation method of a solid probiotic preparation, which specifically comprises the following steps:

[0029] 1) centrifuging the probiotic fermentation broth at a centrifugation speed of 10,000 rpm and a centrifugation time of 5 minutes to obtain probiotic cells, suspending the probiotic cells in 0.9% normal saline to obtain a probiotic cell suspension;

[0030] 2) Add betaine and hydroxyproline to the probiotic cell suspension at room temperature so that the final concentration of betaine is 0.2%, the final concentration of hydroxyproline is 0.12%, and the temperature of the control cell suspension is - 5°C for 4 hours, adjust the temperature to -20°C for 60 minutes; adjust the temperature to -40°C for 6 hours; return to room temperature;

[0031] 3) Add 6% by weight sorbitol and 6% by weight glucose of the probiotic cell suspension to the probiotic cell suspension, put it into a vacuum freeze dryer for freeze-drying, control the freeze-drying temperature to be -3...

Embodiment 3

[0034] A preparation method of a solid probiotic preparation, which specifically comprises the following steps:

[0035] 1) Centrifuge the probiotic fermentation broth at a centrifugation speed of 9000 rpm and a centrifugation time of 4 minutes to obtain probiotic cells, suspend the probiotic cells in 0.9% normal saline to obtain a probiotic cell suspension;

[0036] 2) Add betaine and hydroxyproline to the probiotic cell suspension at room temperature so that the final concentration of betaine is 0.15%, the final concentration of hydroxyproline is 0.09%, and the temperature of the control cell suspension is - 5°C for 3 hours, adjust the temperature to -18°C for 50 minutes; adjust the temperature to -35°C for 5 hours; return to room temperature;

[0037] 3) Add 5% by weight sorbitol and 5% by weight glucose to the probiotic cell suspension, put it into a vacuum freeze dryer for freeze-drying, control the freeze-drying temperature to -30°C, freeze The drying time is 22 hours. ...

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Abstract

The invention relates to a preparation method of a solid probiotic preparation, and belongs to the field of dairy product manufacturing. In order to overcome the shortages that in the prior art, the death rate of cells in a conventional freeze-drying technology is high and the finished products have safety hazard, the invention provides a preparation method of a solid probiotic preparation and a solid probiotic preparation. According to the preparation method, betaine and hydroxyproline are added into probiotic suspension, then the temperature of the probiotic suspension is gradually reduced so as to enhance the anti-freeze performance of probiotics; then glucose and sorbitol are added, and finally freeze-drying is performed to obtain the solid probiotic preparation. The survival rate of probiotics in the prepared solid probiotic preparation can reach 85% or more and is obviously better than that of probiotics in a solid probiotic preparation prepared by a conventional technology. Thepreparation method has the advantages of simple steps and short processing time, and is suitable for popularization and application.

Description

technical field [0001] The invention relates to a preparation method of a solid probiotic preparation, which belongs to the field of dairy product manufacturing. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting beneficial effects. Beneficial bacteria or fungi in humans and animals mainly include Clostridium butyricum, Lactobacillus, Bifidobacterium, Actinomycetes, and yeast. At present, the most powerful products researched in the world are mainly compound active probiotics composed of the above-mentioned various microorganisms, which are widely used in the fields of bioengineering, industry and agriculture, food safety, and life and health. [0003] Reasonable and effective probioti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 程彦
Owner 广州富诺营养科技有限公司
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