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PM2.5 ventilator-induced lung injury antagonist and preparation method thereof

An antagonist and lung injury technology, applied in food science, application, respiratory diseases, etc., to achieve the effect of reducing cell apoptosis, high efficiency, and broad application prospects

Inactive Publication Date: 2018-11-16
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

FMP-1, a homogeneous polysaccharide component extracted from Morchella fruiting bodies in the laboratory, has good antioxidant activity, and it has good anti-oxidative activity for antagonizing PM 2.5 Lung injurious activity has not been reported
In addition, studies have shown that chemical modification of polysaccharides can improve their biological activity and generate new activities by introducing new chemical groups. The common chemical modification methods of polysaccharides are sulfation, carboxymethylation, acetylation, phosphorylation and Alkylation, etc., through appropriate molecular modification to obtain high-efficiency polysaccharide-based PM 2.5 Antagonists have not been reported

Method used

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  • PM2.5 ventilator-induced lung injury antagonist and preparation method thereof
  • PM2.5 ventilator-induced lung injury antagonist and preparation method thereof
  • PM2.5 ventilator-induced lung injury antagonist and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] PM in this example 2.5 The preparation method of the lung injury antagonist is as follows:

[0033] 1. Take 100mg of dried morel polysaccharide FMP-1 in a clean and dry three-necked flask, add 5mL formamide, water bath at 25℃ for 30min, then add 200mg SO 3 -Py, stirred in an oil bath at 70°C for 3h;

[0034] 2. Adjust the pH value of the reaction solution obtained in step 1 with 4mol / L NaOH solution to neutral, place it in a 3500Da dialysis bag, and dialyze for 48h under running water. The resulting dialysate is concentrated, and four times the volume of 95vt% ethanol is added at 4℃. After standing for 12h, centrifuging and collecting the precipitate, the obtained precipitate was freeze-dried (temperature -50°C, air pressure 0.1mbar, time 72h) to obtain the morel sulfated polysaccharide SFMP-1. The results of SFMP-1 see figure 1 (HPLC detection chart) and figure 2 (Infrared spectra).

Embodiment 2

[0035] Example 2: Antagonize PM 2.5 Damage and protection of alveolar cells macrophages experimental test

[0036] 1. Establishment of NR8383 cell injury model

[0037] Wait until the cells grow to 60~80%, adjust the cell suspension to 1×10 5 Plant the density of cells / well into a 96-well plate, add 50μL of medium to each well, culture for 24h; weigh 15mg of PM 2.5 Sample, suspended in 3mL PBS, made 5mg / mL PM 2.5 The suspension is uniformly dispersed by ultrasound, and sterilized at 120°C for 1 hour in a sterilizer, and PM is used each time 2.5 Disperse the sample ultrasonically for 10 minutes before; separate the PM 2.5 The mother liquor was diluted to a concentration of 50, 100, 200, 400, 600, 800 μg / mL and added to a 96-well plate for 4 hours; 4 hours later, 10 μL of MTT solution at a concentration of 5 mg / mL was added to each well for 4 hours; after 4 hours, 100 μL of acidification was added to each well Isopropanol (SDS, isopropanol, 10mM hydrochloric acid) dissolved the produc...

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PUM

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Abstract

The invention discloses a PM2.5 ventilator-induced lung injury antagonist and a preparation method thereof. The antagonist is acquired by performing sulfation modification on Morchella sporocarp polysaccharide, and mainly comprises mannose, glucose and galactose, the molecular weight is 9.0kDa, and the sulfate radical substitution degree is 1.36. The polysaccharide ingredient SFMP-1 acquired by the invention has a good protection effect for the alveolar macrophages injured by PM2.5, and is free from obvious toxic and side effects; the antagonist has a good PM2.5 injury antagonistic effect, andhas huge application prospects in medicine and health care products and other fields.

Description

Technical field [0001] The present invention relates to a PM 2.5 A lung injury antagonist and a preparation method thereof belong to the field of biotechnology. Background technique [0002] In recent years, with the rapid development of my country's economy and the large-scale use of fossil fuels, we are facing and suffering a severe test-air pollution. Which contains PM 2.5 . PM 2.5 , Also known as fine particles, it refers to a particle with a diameter less than or equal to 2.5μm. my country's current PM 2.5 The level is eight times higher than the normal value recommended by the World Health Organization and is considered to be the world’s PM 2.5 One of the highest level countries. Especially in the North China Plain, frequent haze weather has brought serious disasters to people's lives and travel. Due to PM 2.5 The size of the particle, it can enter the human lungs and blood, cause serious effects on the biological system, and even destroy human health. According to the ...

Claims

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Application Information

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IPC IPC(8): A61K31/737A61P11/00C08B37/00A23L33/125
CPCA23L33/125A23V2002/00A61K31/737A61P11/00C08B37/006A23V2200/314A23V2250/51
Inventor 陆永明李婉蔡正楠陈彦
Owner ANHUI UNIVERSITY
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