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Basic medium for plant tissue culture

A technology for plant tissue culture and basic medium, which is applied in plant regeneration, botanical equipment and methods, horticulture, etc., can solve problems such as being unsuitable for promotion and lack of versatility, and is convenient for large-scale promotion and use with good safety. Effect

Active Publication Date: 2018-11-16
SHANDONG AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, CN102224802A strawberry proliferation medium discloses a medium that can replace ammonium nitrate, but this medium is only suitable for strawberry proliferation medium, does not have versatility, and is not suitable for promotion

Method used

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  • Basic medium for plant tissue culture
  • Basic medium for plant tissue culture
  • Basic medium for plant tissue culture

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1: Tobacco leaf callus induction and bud regeneration culture

[0034] Tobacco leaf callus induction and shoot regeneration medium is: ZS basic medium + 6-BA 3mg / L + NAA0.2mg / L + sucrose 30g / L + agar powder 8g / L, pH 5.8. The composition of ZS basic medium is shown in Table 5 (the rest is distilled water, pH 5.5-6.0)

[0035] The production method is as follows: Based on the ZS basic medium, add 6-benzyladenine (BA), naphthaleneacetic acid (NAA), sucrose and agar powder respectively, mix well, and use 1mol / L KOH or 1mol / L HCl Adjust the pH to 5.8; add 3 mg 6-BA, 0.2 mg NAA, 30 g sucrose and 8 g agar powder to each 1 L of ZS basic medium.

[0036] Utilize above-mentioned plant tissue culture medium of the present invention to carry out tobacco callus induction and bud differentiation, specifically: take tobacco leaves, sterilize with 70% ethanol for 30 seconds, then use 0.1% HgCl 2 Disinfect for 8-10 minutes, rinse with sterile water for 4 times, and cut into sm...

Embodiment 2

[0040] Embodiment 2: Tobacco tissue culture seedling takes root

[0041] The rooting medium of tobacco tissue culture seedlings is: 1 / 2ZS basic medium + 20g / L sucrose + 8g / L agar powder, pH is 5.8.

[0042] The production method is as follows: based on 1 / 2ZS basic medium, add sucrose and agar powder respectively, mix well, adjust the pH to 5.8 with 1mol / L KOH or 1mol / L HCl; every 1L of 1 / 2ZS basic medium Add 20g sucrose and 8g agar powder. The composition of ZS basic medium is shown in Table 5 (the rest is distilled water, pH 5.5-6.0)

[0043] Utilize above-mentioned plant tissue culture medium of the present invention to carry out the rooting of tobacco tissue culture seedling, specifically: when the regenerated bud of tobacco grows to 1cm high, cut off and inoculate in the above-mentioned rooting medium and cultivate; culture condition is: 16 hours light , the light intensity is 2000-3000LX, the temperature is 25-26°C; 8 hours of darkness, the temperature is 22-23°C; light...

Embodiment 3

[0047] Embodiment 3: Potato test-tube plantlet cultivation

[0048] Potato test-tube seedling medium is: ZS basic medium + white sugar 30g / L + agar powder 6g / L, pH 5.8.

[0049]The production method is as follows: based on the ZS basic medium, add sugar and agar powder respectively, mix well, adjust the pH to 5.8 with 1mol / L KOH or 1mol / L HCl; add 30g white sugar to every 1L of ZS basic medium and 6g agar powder. The composition of ZS basic medium is shown in Table 5 (the rest is distilled water, pH 5.5-6.0)

[0050] Utilize above-mentioned plant tissue culture medium of the present invention to carry out the cultivation of potato plantlet in test tube, specifically:

[0051] Select the virus-free potato seedlings that are growing vigorously. On the ultra-clean workbench, cut the stem segments to be relatively consistent in size, each stem segment has 1-2 leaf buds, insert them into the medium, and connect 15 stem segments to each culture bottle. Placed in a culture room (i...

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Abstract

The invention discloses a basic medium ZS for plant tissue culture and relates to the field of plant propagation. Every 1000 mL of the basic medium ZS contains 3075 mg of potassium nitrate, 578 mg ofammonium phosphate, 491 mg of calcium nitrate, 160 mg of ammonium chloride, 222 mg of magnesium nitrate, 396 mg of ammonium sulfate, 0.83 mg of potassium iodide, 6.2 mg of boric acid, 22.3 mg of manganese sulfate, 8.6 mg of zinc sulfate, 0.25 mg of sodium molybdate, 0.025 mg of copper sulfate, 0.025 mg of cobalt chloride, 100 mg of inositol, 0.1 mg of thiamine hydrochloride (VB1), 0.5 mg of pyridoxine hydrochloride (VB6), 0.5 mg of nicotinic acid, 37.3 mg of ethylenediaminetetraacetic acid disodium salt and 27.8 mg of ferrous sulfate. By means of the basic medium, the same tissue culture effect as an MS medium can be obtained without ammonium nitrate.

Description

technical field [0001] The invention relates to the field of plant tissue culture rapid propagation, in particular to a basic medium for plant tissue culture. Background technique [0002] Murshige and Skoog medium (MS, 1962) is a plant tissue culture medium widely used at present, and ammonium nitrate is an important component of the medium. Ammonium nitrate is a strong oxidizing agent, which can fuel the fire when combustibles catch fire; when mixed with combustible powder, it can react violently and explode; it can also explode when subjected to strong vibration or rapid heating. It can form an explosive mixture when mixed with reducing agents, organic substances, flammable substances such as sulfur, phosphorus or metal powder, and is the raw material for making explosives. Because of the huge potential safety hazards, in recent years, the national supervision of such items has become more and more strict, and the market circulation has been restricted by the relevant st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 朱常香宋云枝张晓英王洪凤曹伟琳
Owner SHANDONG AGRICULTURAL UNIVERSITY
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