Enzyme-free glucose electrochemical sensor and detection method thereof

Abstract

The invention discloses an enzyme-free glucose electrochemical sensor and a detection method thereof. The enzyme-free glucose electrochemical sensor comprises a three-electrode system consisting of areference electrode, a counter electrode and a working electrode, wherein the working electrode is made of a material with electrocatalytic oxidation performance for glucose; the method for detectingglucose by using the enzyme-free glucose electrochemical sensor comprises the following steps: (1) electrochemical pretreatment of the working electrode: applying high negative potential; (2) electrochemical oxidation of glucose: applying the potential required for glucose oxidation; (3) electrochemical cleaning of the working electrode: applying positive potential. The influence of pH of a sampleon a detection result can be removed, enzyme-free glucose detection originally required under the alkaline condition can be performed in neutral and acidic samples, and the sensor has the advantagesof good electrode stability, high sensitivity, good repeatability and the like, and has a very broad application prospect.

Description

technical field [0001] The invention relates to a glucose detection device and a detection method thereof, in particular to an enzyme-free glucose electrochemical sensor and a detection method thereof. Background technique [0002] Glucose is the most widely distributed and most important monosaccharide in nature, and it plays an important role in the field of biology. The development of high-sensitivity glucose detection technology is of great significance to the study of biological metabolism and diagnosis of diseases. Electrochemical detection method has attracted more and more attention due to its high sensitivity, simple operation, easy miniaturization and real-time online. Glucose detection based on electrochemical technology has also become a hot research topic for researchers. [0003] Glucose detection usually includes two methods: enzyme detection and non-enzyme detection. Among them, enzyme detection is the main method for in vitro detection of glucose. By adding...

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Problems solved by technology

[0003] Glucose detection usually includes two methods: enzyme detection and non-enzyme detection. Among them, enzyme detection is the main method for in vitro detection of glucose. By adding highly specific glucose oxidase or dehydrogenase during the detection process, a large amount of glucose can be eliminated. However, glucose oxidase is not stable, its catalytic activity is affected by ambient temperature, humidity and pH, and its stability is tested with the increase of storage time, although it has been Many methods have been reported to improve the stability of enzymes, but on the one hand, these operations are tedious and time-consuming, on the other hand, the repeatability of the experiment is reduced, and they are still limited by pH and temperature; non-enzyme detection is the process of glucose and catalysis in the detection process. Electron transfer occurs directly between electrodes, thus getting rid of the dependence on enzymes, compared with enzyme detection, enzyme-free detection has higher sensitivity and long-term stability

However, the enzyme-free electrodes in the prior art must be used under strong alkaline conditions to obtain high sensitivity. Under neutral and acidic conditions, the electrodes are easily adsorbed by oxidation products and poisoned by environmental ions, which greatly limits its practical application.

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