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Specific molecular marker PCMI-M001 for rapid identification of male seedlings of populus cathayana

A molecular marker and specific technology, which is applied in the field of molecular identification of economic forest trees, can solve the problems of inaccurate detection results and large influence of detection results, and achieve good economic value, shorten the breeding cycle, and simple methods

Active Publication Date: 2018-11-13
HANGZHOU NORMAL UNIVERSITY
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Problems solved by technology

Traditional identification methods are mainly through morphological observation, physiological and biochemical detection, and amino acid content difference detection, etc., mainly for the detection of adult plants, and human factors have a greater impact on the detection results, resulting in inaccurate detection results

Method used

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  • Specific molecular marker PCMI-M001 for rapid identification of male seedlings of populus cathayana
  • Specific molecular marker PCMI-M001 for rapid identification of male seedlings of populus cathayana
  • Specific molecular marker PCMI-M001 for rapid identification of male seedlings of populus cathayana

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Experimental program
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Embodiment 1

[0023] 1. Genomic DNA extraction of Poplar to be tested

[0024] Take 0.2-0.5g of the fresh leaves of Populus pubescens to be tested, add liquid nitrogen and grind them into powder in a mortar, and then use the Ezup column plant genome DNA extraction kit of Shanghai Bioengineering Technology Co., Ltd. to extract the samples of Populus pubescens to be tested Genomic DNA, use 1% agarose gel to electrophoresis the obtained DNA to check the purity, and use the NanoDropTM 1000 ultra-micro ultraviolet spectrophotometer of Thermo Fisher Corporation of the United States to detect the DNA concentration, and then dilute to 20-50ng / μL , 4 degrees refrigerator for later use.

[0025] 2. Synthesis of Specific Molecular Marker Primers

[0026] Design specific molecular marker primers based on the sex difference sequence of Populus pubescens,

[0027] Upstream primer PCMI-F001-F: 5'-TACCACTGCGGGGAAGGAAA-3';

[0028] Downstream primer PCMI-F001-R: 5'-CACTGCGCAAGAAGCAGTAA-3';

[0029] Synt...

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Abstract

The invention discloses a specific molecular marker PCMI-M001 for rapid identification of male seedlings of populus cathayana. A nucleotide sequence of a forward primer PCMI-M001-F of the molecular marker PCMI-M001 is shown as SEQ ID NO:1; a nucleotide sequence of a reverse primer PCMI-M001-R of the molecular marker PCMI-M001 is shown as SEQ ID NO:2; a nucleotide sequence of a specific band obtained by amplification of the marker in a male individual is shown as SEQ ID NO:3. The specific molecular marker (PCMI-M001-F / R) can be used for rapid molecular identification of male and female populuscathayana, and a method is simple, accurate and high in sensitivity and superior to other existing identification methods.

Description

technical field [0001] The invention belongs to the technical field of molecular identification of economic forest trees, and in particular relates to a specific molecular marker PCMI-M001 for rapid identification of poplar male seedlings. Background technique [0002] Populus cathayana is a dioecious plant of the genus Populus in the family Salicaceae. As a common economic and ecological restoration tree species in China, it is mainly distributed in North China, Northwest China, Liaoning, Sichuan and other regions of China. Strong and easy to reproduce, and its wood is light and soft, the wood texture is linear, and the structure is fine. It is suitable for economical materials such as furniture, wooden boxes, and buildings, as well as windproof and sand-fixing, greening environment, etc. It has both economic development and ecological environment. Significance. At present, researchers have found that the female P. chinensis grows adjacent to the female P. chinensis, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6879C12N15/11
CPCC12Q1/6879C12Q1/6895C12Q2600/156
Inventor 卢江杰亢介玉刘玉洋刘朋丽李春阳
Owner HANGZHOU NORMAL UNIVERSITY
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