Positive circulating tumor cell enrichment method
A tumor cell positive technology, applied in the field of positive enrichment of circulating tumor cells, can solve problems such as difficult to ensure specificity and recovery rate at the same time, analysis troubles, affecting the accuracy, sensitivity and specificity of tumor cell detection, to avoid Effects of irreversible damage, maintenance of cell viability, and broadening of utility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0058] In order to verify the capture ability of this enrichment method for different cell lines, the MCF-7 and NCI-H226 cell lines pre-labeled with Cell Tracker GreenCMFDA were respectively selected, and after accurate counting, they were mixed into the peripheral blood of healthy volunteers and carried out according to this method. Enrichment, statistical recovery, the specific operation method is as follows:
[0059] 1. PBMC blood separation: Before the experiment, take out the sample density separation solution and equilibrate to room temperature, add 3mL sample density separation solution to the lymphatic separation tube in advance, then add 3mL PBS and 3mL peripheral blood of healthy volunteers in order, and add accurately counted Cell Tracker Green CMFDA pre-labeled MCF-7 and NCI-H226 cells, 2000rpm, centrifuge for 20min; use a Pasteur pipette to extend into the buffy coat (mononuclear cell enrichment area) to absorb, transfer to a new centrifuge tube, discard The red b...
Embodiment 2
[0072] In order to verify the possibility of using this method to isolate and obtain target cells in vitro, the peripheral blood samples of lung cancer patients were used to separate circulating tumor cells, and relevant technical means were used to obtain stable proliferating cell lines. The specific operation methods are as follows:
[0073]1. PBMC blood separation: Before the experiment, take out the sample density separation solution and equilibrate to room temperature, add 3mL sample density separation solution to the lymphatic separation tube in advance, then add 3mL PBS and 3mL peripheral blood of lung cancer patients in sequence, centrifuge at 2000rpm for 20min; use Insert the Pasteur pipette into the buffy coat (the mononuclear cell-enriched area) to absorb and transfer to a new centrifuge tube, and discard the red blood cell layer at the bottom of the lymphatic separation tube. Centrifuge at 300g for 10min, discard the supernatant;
[0074] 2. According to 1 volume o...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com