A bacterial strain capable of degrading discarded feathers, a screening method and its application

A technology for strains and feathers, applied in the field of microorganisms, can solve the problems of damage to the nutritional value of feather meal, loss of nutrients, and pollution of the environment, and achieve the effects of improving absorbability, avoiding the loss of amino acids, and high degradation efficiency.

Active Publication Date: 2021-08-27
XINYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can promote the digestion and utilization of feathers by animals to a certain extent, there are inherent defects in these processes, such as high energy consumption and environmental pollution.
More importantly, after these processes, the nutritional value of the obtained feather meal has been destroyed to a certain extent, because those unstable components will deteriorate during the process, resulting in the loss of nutrients

Method used

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  • A bacterial strain capable of degrading discarded feathers, a screening method and its application
  • A bacterial strain capable of degrading discarded feathers, a screening method and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Screening and molecular identification of bacterial strains that can efficiently degrade discarded feathers

[0030] Taking tea garden soil air-dried for 4 months as a sample, the high-efficiency feather-degrading strain Bacillus cereus FDB-18B was obtained through the steps of primary screening, secondary screening, obtaining pure culture, quantitative determination of feather degradation ability, molecular identification, and strain preservation. The specific screening steps are as follows:

[0031] (1) Sampling: Take 4 g of tea garden soil samples air-dried for 4 months, dissolve them in 40 ml of sterilized sterile water, vortex and oscillate fully, and let stand for 5 minutes to obtain a soil suspension;

[0032] (2) Primary screening: take 1ml of the soil suspension, add it to the primary screening medium (just after sterilization, the temperature is not lower than 90°C), and cultivate it at 37°C and 150rpm for 2 days to obtain a mixed solution;

[0033...

Embodiment 2

[0044] Embodiment 2: the application of the bacterial strain Bacillus cereus FDB-18B that can efficiently degrade discarded feathers

[0045] Take out the frozen strain tube from the refrigerator at -80°C, place it in warm water at 37°C immediately, let it melt rapidly, take 10 μl and inoculate it into 10ml of sterilized LB liquid medium, at 37°C (150rpm) Incubate overnight (about 15 h) in a shaker. According to the ratio of 1:50-100, inoculate the bacterial solution into a new Erlenmeyer flask containing 50ml of LB liquid medium, and cultivate it in a shaker at 37°C (150rpm) to the logarithmic phase. Inoculate 0.007-0.06 (OD600) into the fermentation medium containing intact feathers, continue culturing at 37°C and 150 rpm to degrade the feathers, and take pictures to record the changes of the intact feathers after 24 hours of degradation. The results are shown in figure 1 .

[0046] The specific components of the feather fermentation medium are: sodium chloride 1.7mM, dipo...

Embodiment 3

[0048] Embodiment 3: Optimization of the application of the bacterial strain Bacillus cereus FDB-18B that can efficiently degrade discarded feathers

[0049] Take out the frozen strain tube from the refrigerator at -80°C, place it in warm water at 37°C immediately to make it melt quickly, take 10 μl and inoculate it into 10 ml of sterilized LB liquid medium, and inoculate at 37°C, 150rpm Cultivate overnight (about 15h) in a shaker. According to the ratio of 1:50-100, inoculate the bacterial solution into a new Erlenmeyer flask containing 50ml of LB liquid medium, and cultivate it in a shaker at 37°C (150rpm) to the logarithmic phase. 0.015 (OD600) was inoculated into the fermentation medium containing complete feathers (the concentration of added soluble starch was 0, 1.25%, 2.5%, 5%, 10%, weight / volume ratio, wherein, the concentration of added soluble starch was 0 As the control group and the rest as the experimental group), the culture was continued at 37° C. and 150 rpm t...

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Abstract

The invention relates to a bacterial strain capable of degrading discarded feathers, belonging to the technical field of microorganisms. The strain is Bacillus cereus FDB‑18B, which was deposited in the China Center for Type Culture Collection with the date of preservation on June 13, 2018, and the preservation number is CCTCC NO.2018365. The bacterial strain of the present invention is obtained from air-dried tea garden soil, which can efficiently degrade discarded feathers and obtain amino acids and polypeptides that can be used as feed additives or farmland irrigation, realize comprehensive utilization of biomass resources, and reduce poultry breeding at the same time. pollution of the environment. In addition, the strain can produce amylase, which can simultaneously realize the comprehensive treatment of waste feathers and waste water produced in starchy food processing. Due to the strong degradation ability of the bacteria and the short fermentation cycle, the protein, polypeptide and amino acid components are kept to the greatest extent, and the nutritional value and utilization rate as feed additives can be improved.

Description

technical field [0001] The invention relates to a bacterial strain capable of degrading discarded feathers, belonging to the technical field of microorganisms. Background technique [0002] Feather waste from poultry production is considerable. It is estimated that in the case of China, the annual output can also be as high as 1 million tons. Although the main component of feathers is protein, the content of keratin is as high as about 90%, so it is difficult to be degraded, and it is not easy to be digested by the digestive enzymes in the intestinal tract of animals. Therefore, it has not been effectively used for a long time. This not only causes the waste of these available biological resources, but also brings about the problem of environmental pollution. [0003] With the improvement of Chinese people's living standards in recent years, the proportion of animal food has been increasing, and the scale of poultry farming has also been further expanded, making the resul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/12C12R1/085
CPCA23K10/12C12N1/20C12N1/205C12R2001/085
Inventor 王天文梁辰高婉茹高倩罗欣琪
Owner XINYANG NORMAL UNIVERSITY
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