Method for preparing marine animal ink melanin

Abstract

The invention relates to a method for preparing marine animal ink melanin. The method for preparing the marine animal ink melanin includes the steps of extracting a cephalopod ink sac for ultrasonic treatment to obtain ink, adding the ink to a protease solution for hydrolysis, and performing ultrafiltration membrane filtration of the hydrolyzed protease solution after heating to obtain of ink melanin finished products of different molecular weights. The method for preparing the marine animal ink melanin has various advantages such as that with the above-mentioned technical scheme of adopting ultrasonic and enzymatic extraction of the soluble ink melanin and separation of high, medium and low molecular weight ink melanin by ultrafiltration membrane, the method is simple and easy to operate;with ultrasound and directional thermal enzymatic hydrolysis used to extract the soluble ink melanin, ultrasound is helpful for directional separation of proteins and ink melanin from an ink sac; with the entire ink sac placed in the ultrasound for processing, wall hanging phenomenon is reduced, and the yield of ink is increased when the ink is being squeezed out; enzymatic extraction conditionsare mild, which is conductive to maintaining the stability of the morphological structure of natural ink melanin; ultrafiltration membrane technology can be adopted to obtain the soluble ink melanin of different molecular weights with short time consuming and high extraction rate; the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method of black pigment, in particular to a preparation process of marine animal black pigment. Background technique [0002] The word melanin comes from Greece and was first proposed by the Swiss chemist Berzelius. It is a water-insoluble biomacromolecule polymer and the only known natural endogenous organism that can protect organisms from radiation damage. Polymers, which are widely present in animals and plants. Marine animal ink melanin is mainly distributed in the mollusca (Mollusca) cephalopod (Cephalopoda). Ink melanin is a synonym for the biomacromolecular polymer contained in the ink of marine cephalopods such as cuttlefish, squid, and octopus. It is the only known natural endogenous biopolymer that can protect organisms from radiation damage. Contains various bioactive ingredients such as polypeptide fragments, acidic polysaccharides, tyrosinase, etc. With the characteristics of scavenging free rad...

AI Technical Summary

Problems solved by technology

At present, the extraction methods of marine cephalopod melanin mainly include high-speed centrifugation, acid / alkali hydrolysis, enzymatic hydrolysis, alkali-dissolving acid precipitation, etc., but there are some shortcomings and deficiencies in the existing extraction methods: high-speed centrifugation The purity of the black melanin obtained is not high; the purity of the black melanin prepared by the acid-base hydrolysis method is higher, but the hydrolysis process is too severe, which will destroy the melanin, which is not conducive to maintaining the completeness and biological activity of the melanin; the traditional enzymatic hydrolysis method The structure of melanin is complete, but it is impossible to separate melanin from other impurities that are not decomposed by proteins; the melanin extracted by alkali-soluble acid precipitation method has a complete structure and high purity, but it cannot extract melanin combined with polysaccharides and proteins, and the extraction rate is relatively low. Low