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A method for identification of chemical constituents in guava leaves

A guava leaf and identification method technology, applied in scientific instruments, material separation, analysis of materials and other directions, can solve problems such as inability to quickly and accurately determine objects, and achieve improved purity and identification efficiency, high sensitivity, and strong accuracy. Effect

Active Publication Date: 2021-04-27
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods cannot quickly and accurately determine all objects, and all have certain limitations.

Method used

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  • A method for identification of chemical constituents in guava leaves

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Effect test

Embodiment 1

[0027] A kind of identification method of chemical composition in guava leaf of the present invention, comprises the steps:

[0028] (1) Take the guava leaf powder and place it in a 150m L conical flask respectively, and use a volume concentration of 60% ethanol solution to extract the guava leaf powder, and the liquid-solid ratio of the ethanol solution and the guava leaf powder is 20mL : 1g, the extraction temperature is 85°C, the extraction time is 2.0h, and the extract is collected after filtering the residue with a microporous membrane with a filter aperture of 0.22mm; under this process condition, polyphenols, flavonoids in guava leaves and the extraction rates of terpenes were 22.20%, 6.54% and 1.04% respectively;

[0029] (2) the extract obtained after the step (1) is separated by ultra-high performance liquid chromatography, and the ultra-high performance liquid chromatography adopts Agilent SB-C8 chromatographic column (specification 150mm * 4.6mm, 3.5 μ m), Carry o...

Embodiment 2

[0034] A kind of identification method of chemical composition in guava leaf of the present invention, comprises the steps:

[0035] (1) Take the guava leaf powder and place it in a 150m L conical flask respectively, and use a volume concentration of 50% ethanol solution to extract the guava leaf powder, and the liquid-solid ratio of the ethanol solution and the guava leaf powder is 10mL : 1g, the extraction temperature is 95°C, the extraction time is 3.0h, and the extract is collected after filtering the residue with a microporous membrane with a filter aperture of 0.22mm; under this process condition, polyphenols, flavonoids in guava leaves The extraction rates of terpenoids and terpenoids were 19.34%, 4.59% and 0.83% respectively; too high temperature would increase the viscosity of the extract and hinder the dissolution of the active substances, thereby slightly reducing the extraction rate.

[0036] (2) the extract obtained after the step (1) is separated by ultra-high pe...

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Abstract

The invention discloses a method for identifying chemical components in guava leaves, which comprises the following steps: using ethanol solution to leach guava leaf powder, filtering the residue with a microporous membrane and collecting the extract; the obtained extract is obtained by Ultra-high performance liquid chromatography was used to separate and obtain different fractions; time-of-flight mass spectrometry was used to collect data, and the chemical composition was identified by combining the primary mass spectrum, secondary mass spectrometry, isotope distribution and compound cracking rules of the target compound. The identification method of the present invention adopts an organic solvent precipitation method to extract the chemical components in the guava leaves, which is simple to handle and easy to operate, and does not require complicated extraction steps; the chemical components of the guava leaves are separated by ultra-high performance liquid chromatography, The purity of chemical components and the efficiency of identification are greatly improved, the detection time is short, and the detection limit, precision and recovery are excellent; the data is collected by mass spectrometry, with high sensitivity and high accuracy.

Description

technical field [0001] The invention belongs to the field of guava leaf processing, in particular to a method for identifying chemical components in guava leaves. Background technique [0002] Guava leaves are the dried leaves of Guava, a plant of the genus Guava in the family Myrtaceae, and its chemical components are mainly polyphenols, flavonoids and triterpenes. and other active effects. These chemically active ingredients have the physiological effects of anti-oxidation, preventing or inhibiting tumors, anti-bacteria, anti-atherosclerosis, lowering blood fat, preventing or treating cardiovascular and cerebrovascular diseases such as coronary heart disease and stroke. Therefore, extracting and identifying biologically active substances from guava leaves with the help of various technical means has become a current research hotspot. [0003] At present, the determination methods of plant polyphenols, flavonoids and triterpenoids mainly include spectrophotometry (UV), hi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 胡芳曾伟民彭玙萍周智广吴学玲李交昆申丽余润兰刘元东谢志国邱冠周
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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