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Staining groove staining method

A dyeing method and dyeing tank technology, applied in the field of dyeing tank dyeing, can solve the problems that the internal structure is not clear enough, do not see the use of hydrochloric acid alcohol differentiation operation, etc., and achieve the effect of clear internal structure of the nucleus

Inactive Publication Date: 2018-11-06
泰普生物科学(中国)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The existing dyeing vat staining technology generally includes the hydrochloric acid alcohol differentiation step, but the existing staining tank staining technology has not seen the use of hydrochloric acid alcohol differentiation operation, especially for small cells, the internal structure of the cell nucleus is not clear enough after staining The problem

Method used

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Experimental program
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Embodiment 1

[0035] In the cell staining method of this embodiment, the original staining vat staining technique is improved to form a new staining tank staining technique. The specific feature is to use the staining tank to fix the staining area, the inner diameter of the staining tank is less than 25 mm, the height is variable, and the slide is still. After not moving, the cell preparations were stained with hematoxylin, and then differentiated with hydrochloric acid and alcohol. Specifically: add washing solution, buffer solution, hematoxylin, buffer solution, hydrochloric acid alcohol solution, and buffer solution sequentially by manual or mechanical means, that is, the first washing solution treatment, the first buffer solution treatment, hematoxylin treatment, and the second treatment are performed in sequence. Secondary buffer treatment, hydrochloric acid alcohol solution treatment and third buffer treatment, the specific process and time parameters are as follows:

[0036] Washing ...

Embodiment 2

[0041] The cell staining method of this embodiment is only different from the first embodiment in the following process, and the others are the same as the first embodiment.

[0042] The processing times of the first washing liquid treatment, the first buffer treatment, the hematoxylin treatment, the second buffer treatment, the hydrochloric acid alcohol solution treatment and the third buffer treatment are 49s, 46s, 115s, 46s, 3s and 38s. The processing times of the fourth buffer treatment, the second washing solution treatment, the EA / OG staining solution treatment, the third washing solution treatment and the fourth washing solution treatment are 38s, 46s, 115s, 37s and 47s in sequence .

Embodiment 3

[0044] The cell staining method of this embodiment is only different from the first embodiment in the following process, and the others are the same as the first embodiment.

[0045] The processing time of the first washing liquid treatment, the first buffer treatment, the hematoxylin treatment, the second buffer treatment, the hydrochloric acid alcohol solution treatment and the third buffer treatment is 52s, 49s, 125s, 50s, 7s and 42s. The processing time of the fourth buffer solution treatment, the second washing solution treatment, the EA / OG staining solution treatment, the third washing solution treatment and the fourth washing solution treatment is 42s, 50s, 125s, 41s and 51s in sequence .

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Abstract

The invention relates to a staining groove staining method. staining operation is conducted on a static cell preparation in a staining groove, and the staining operation specifically comprises the steps that first washing liquid processing, first buffering liquid processing, hematoxylin staining liquid processing, secondary buffering liquid processing, acid alcohol processing and third buffering liquid processing are sequentially conducted on the cell preparation. The staining groove staining method has the advantage that clear internal structure of a cell nucleus after the cell nucleus is subjected to hematoxylin staining can be guaranteed.

Description

technical field [0001] The invention relates to exfoliated cell staining technology, wherein hematoxylin is used for nuclear staining, and EAOG (abbreviated as Papanicolaou staining) or eosin (abbreviated as HE staining) is used for patina staining, in particular to a dyeing tank dyeing method carried out in a fixed dyeing tank. Background technique [0002] The existing liquid-based cytology detection technology is to make slices of exfoliated cells in clinical specimens, and then use Pap or HE staining to observe the fine structure of the nucleus and the morphology of the cytoplasm under an optical microscope, so as to make a morphological analysis of the cells. Check and distinguish abnormalities, which requires that the internal structure of the cell nucleus must be clear after staining. [0003] At present, there are two types of dyeing techniques, which are referred to as vat dyeing technology and slot dyeing technology respectively. Existing staining vat staining tec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 周晓强谢洪文王贤
Owner 泰普生物科学(中国)有限公司
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