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High temperature-resistant bacterium for promoting ammonia assimilation function in sludge composting process

An assimilation, high temperature-resistant technology, applied in bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as nitrogen loss, limiting the application and promotion of sludge composting, affecting the air quality of production workshops, etc.

Active Publication Date: 2018-11-06
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sludge composting is one of the mainstream technologies for sludge stabilization and disposal. However, during the sludge composting process, a large amount of nitrogen leaves the material system in the form of ammonia and enters the atmosphere, which not only leads to the loss of nitrogen in the composting process, but also reduces the quality of composting products. , It also affects the air quality of the production workshop and the surrounding environment of the factory area, so the volatilization of ammonia gas limits the application and promotion of sludge composting

Method used

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  • High temperature-resistant bacterium for promoting ammonia assimilation function in sludge composting process
  • High temperature-resistant bacterium for promoting ammonia assimilation function in sludge composting process
  • High temperature-resistant bacterium for promoting ammonia assimilation function in sludge composting process

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specific Embodiment approach 1

[0014] Specific Embodiment 1: In this embodiment, Bacillus mojavensis P2 is isolated from the sewage treatment sludge of Harbin Wenchang Sewage Plant.

[0015] Isolation and purification of Bacillus mojavensis P2:

[0016] Take 1.0g sample of sewage plant sludge composting in the high temperature period, put it in 100mL sterile water, add glass beads and shake it well, take the supernatant to dilute in sequence, and then take 10 -6 、10 -7 、10 -8 Spread 100 μL of the diluted solution on the separation medium plate, and incubate in a 50°C incubator for 72 hours. After colonies are visible to the naked eye, pick a single colony for further purification;

[0017] The purified single colony was picked and inserted into the sludge extract medium to detect the ability to assimilate ammonia nitrogen, and the strain with the strongest ammonia assimilation ability was selected as P2. (The same inoculum size, the same culture time and different strains on the NH in the medium 4 + Th...

Embodiment 1

[0024] compost:

[0025] One, Bacillus mojavensis (Bacillus mojavensis) P2 is inoculated into liquid medium A in test tube (liquid medium A comprises 0.25% sodium acetate, 0.05% ammonium nitrate, 0.025% ferric ammonium citrate, 0.1 % sodium chloride, 0.25% dipotassium hydrogen phosphate, 0.05% dipotassium hydrogen phosphate, 0.025% magnesium sulfate and 0.005% manganese sulfate) for 24 hours, and then insert it into the Erlenmeyer flask with 1% inoculum Continue to expand the culture for 24 hours to form a bacterial suspension.

[0026] Wherein, all the culture medium for the expanded culture in the Erlenmeyer flask is liquid medium A.

[0027] 2. Add pumice with 50% fresh sludge quality to the fresh sludge of Harbin Wenchang Sewage Treatment Plant (taken from July 4, 2016) with a humidity of 80%, mix well, compost and ferment and force ventilation, the ventilation rate is 0.25 L / min·L, ferment for 48 ± 2 hours, add the Bacillus mojavensis P2 expanded in step 1 according to ...

Embodiment 2

[0030] compost:

[0031] Add pumice with 50% fresh sludge quality to the fresh sludge of Harbin Wenchang Sewage Treatment Plant (taken on July 4, 2016) with a humidity of 80%, mix well, compost and ferment, and force ventilation with a ventilation rate of 0.25L / min·L, 20 days of fermentation to complete the composting and fermentation.

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Abstract

The invention relates to high temperature-resistant bacterium for promoting ammonia assimilation function in the sludge composting process. The high temperature-resistant bacterium is bacilluse mojavensis P2, and is preserved in China General Microbiological Culture Collection Center; the collection number is CGMCC NO. 14980. The high temperature-resistant bacterium has the advantages that the ammonium ions in sludge can be assimilated, the generation amount and volatilization amount of ammonia gas in the sludge are reduced, and the pollution to the environment in the sludge composting processis reduced; the high temperature of 65 DEG C can be resisted.

Description

technical field [0001] The invention relates to a high temperature resistant bacterium. Background technique [0002] With the rapid development of my country's urbanization and the continuous improvement of sewage treatment capacity, the sludge output of urban sewage treatment plants is increasing day by day. Sludge composting is one of the mainstream technologies for sludge stabilization and disposal. However, during the sludge composting process, a large amount of nitrogen leaves the material system in the form of ammonia and enters the atmosphere, which not only leads to the loss of nitrogen in the composting process, but also reduces the quality of composting products. , It also affects the air quality of the production workshop and the surrounding environment of the factory area, so the volatilization of ammonia gas limits the application and promotion of sludge composting. Contents of the invention [0003] The invention provides a high-temperature-resistant bacter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07
CPCC12R2001/07C12N1/205Y02W30/40
Inventor 孟利强张淑梅张先成李晶宫海楠曹旭胡基华姜威刘宇帅陈镜宇
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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