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Method for preserving chlorella

A preservation method and chlorella technology, applied in microorganism-based methods, biochemical equipment and methods, preservation of microorganisms, etc., can solve problems such as inconvenience in aquaculture, avoid low-temperature energy consumption and transportation inconvenience, accelerate release, The effect of reducing processing time

Inactive Publication Date: 2018-11-06
兰溪市沉默生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Marine microalgae are rich in nutrients and contain a large amount of protein, polysaccharides, unsaturated fatty acids, multivitamins and other nutrients, and are widely used in aquaculture. However, the growth of microalgae is easily affected by environmental, climate and other factors, and often It brings great inconvenience to the aquaculture industry, so the research and development of the application of cryobiology in microalgae is getting more and more attention
At present, there is a lack of a preservation method for chlorella with good preservation effect

Method used

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  • Method for preserving chlorella

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Effect test

Embodiment 1

[0022] A method for preserving chlorella, specifically comprising the following steps:

[0023] S1: Inoculate Chlorella in the culture medium for closed culture, the culture light cycle is 10L:14D, the temperature is 20°C, after inoculation and after adding water to expand the species, apply inorganic fertilizer ammonium bicarbonate and superphosphate with a mass ratio of 3: 1. Sprinkle 25g per square meter of water surface evenly in the whole pool, fertilize once a day, monitor the algae cell density and water chemical indicators in the test pool every day, cultivate the microalgae to the logarithmic growth phase, and wait for use;

[0024] S2: Take the chlorella algae liquid in the logarithmic phase, centrifuge it at 500r / min for 15min, remove the supernatant to get the chlorella concentrate, then put it in a microwave oven, adjust the power of the microwave oven to 20% of the maximum power, and dry it to Constant weight, to obtain microwave-dried algae powder;

[0025]S3: ...

Embodiment 2

[0032] A method for preserving chlorella, specifically comprising the following steps:

[0033] Cultivation: inoculate Chlorella in the culture medium for closed culture, the cultivation light cycle is 12L:12D, the temperature is 22°C, after inoculation and after adding water to expand the species, apply the inorganic fertilizer ammonium bicarbonate and superphosphate in a mass ratio of 4: 1. Sprinkle 35g per square meter of water surface evenly in the whole pond, fertilize once a day, monitor the algae cell density and water chemical indicators in the test pond every day, and cultivate the microalgae to the logarithmic growth phase for use; under suitable cultivation conditions Cultivating microalgae strains can promote the healthy growth and reproduction of microalgae strains. Concentration and preservation after cultivation to the logarithmic growth phase is conducive to maintaining the relatively strong vitality of chlorella, and it is easy to improve its activity and survi...

Embodiment 3

[0041] A method for preserving chlorella, comprising: cultivating, concentrating, and preserving, specifically comprising the following steps:

[0042] Cultivation: inoculate Chlorella in the culture medium for closed culture, the culture light cycle is 12L:12D, the temperature is 20°C, after inoculation and after adding water to expand the species, apply inorganic fertilizer with a mass ratio of ammonium bicarbonate to superphosphate of 3.5: 1. Sprinkle 32g per square meter of water surface evenly in the whole pool, fertilize once a day, monitor the algae cell density and water chemical indicators in the test pool every day, and cultivate the microalgae to the logarithmic growth phase for use; under suitable culture conditions Cultivating microalgae strains can promote the healthy growth and reproduction of microalgae strains. Concentration and preservation after cultivation to the logarithmic growth phase is conducive to maintaining the relatively strong vitality of chlorella...

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Abstract

The invention discloses a method for preserving chlorella. The method comprises the following steps of 1, culturing: inoculating the chlorella into a culture solution, and culturing for the logarithmic growth period in a sealing way for further use; 2, concentrating: centrifuging the chlorella solution to obtain a concentrated solution, putting into a microwave oven, and drying to the constant weight, so as to obtain the dried chlorella powder; 3, preserving: adding the dried chlorella powder and distilled water into a cell culture bottle, adding glycerin, golden camellia extract and aloe extract, completely coating the cell culture bottle by tin foil paper, and preserving. The method has the beneficial effects that the operation is simple, and the purities and contents of the preserved mediums of golden camellia extract and aloe extract are higher; the biology activity is higher, the chlorella can maintain the complete cell structure and high survival rate, and the important indexes of biomass, chlorophyll, active polysaccharide and the like are not obviously degraded after reviving; the valid time is long, the activity is high, and the method is sustained.

Description

technical field [0001] The invention relates to the field of algae preservation, in particular to a method for preserving chlorella. technical background [0002] Chlorella ( Chlorella vulgaris ) Chlorophyceae, Chlorellaceae. Unicellular algae, often solitary, but also multicellular aggregates. The cells are spherical and oval, with a perigene, cup-shaped or sheet-shaped pigment body inside. Asexual reproduction, each cell can produce 2, 4, 8 or 16 spore-like spores, when mature, the mother cell ruptures, spores escape, and become new individuals after growing up. It is distributed all over the world, and mostly lives in small shallow water, and there are also seafood species. There are few individuals under natural conditions, and they are reproduced in large numbers in artificial culture. The protein, fat and carbohydrate content in the cells are very high, and there are many kinds of vitamins, which can be eaten and used as bait. [0003] In terms of industry, Japan...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/04C12R1/89
CPCC12N1/04C12N1/12
Inventor 周建波
Owner 兰溪市沉默生物科技有限公司
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