A micro-chamber microfluidic system for microbial growth image detection

Abstract

A micro-chamber microfluidic system for microbial growth image detection is disclosed. The system includes a microscope, a constant current syringe pump, a syringe, a constant-temperature stage, a micro-chamber microfluidic chip, a collector and a CMOS video detection component. The constant-temperature stage is disposed on a microscope stage. The micro-chamber microfluidic chip is arranged on theconstant-temperature stage. A micro-chamber structure having an independent design can be used for real-time microbial culture. The CMOS video detection component is connected and fixed above the microscope, and is used for measuring morphological changes of bacteria in each micro-chamber in the micro-chamber microfluidic chip. The CMOS video detection component also includes a computation unit,and the computation unit is used for acquiring clear gray level image information of microbes based on a linear spatial filtering implementing principle and a corresponding segmentation equation and coefficients. The system effectively overcomes a technical defect that traditional microbial image detection methods are time-consuming, labor-consuming, expensive, single in function, and incapable ofacquiring long-time real-time real microbial growth image information, and has a great application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and more specifically relates to a microchamber microfluidic system for detecting microorganism growth images. Background technique [0002] The cultivation of microorganisms is the basic technology in the fields of life science and medical research, as well as industrial fermentation and food testing. However, in the current field of bacterial culture and detection, only the growth curve of bacteria can be obtained as a detection index, that is, the use of turbidity detection devices, such as Spectrophotometer, microplate reader, etc. to obtain the change curve of bacterial cell density and number. However, with the development of science, technology and economy, this detection technology can no longer meet the requirements of today's scientific research and the detection field of food fermentation industry. Therefore, there is an urgent need for a detection device with high thr...

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Problems solved by technology

[0006] Although microfluidic chips have made some progress in the field of bacteria and cells, there are still many technical defects in the cultivation of microorganisms. For example, the channel culture mode is often used, and the cultivation of a single channel will limit the growth and movement of microorganisms; In addition, the double-layer restricted culture mode is often used, that is, the bottom layer is inoculated with microorganisms, and then the upper layer is covered with a layer of polymer film, and the osmotic culture is carried out by circulating the medium in the upper layer. This method severely limits the movement of microorganisms and the interaction between microorganisms and the medium. Contact is not conducive to the growth of microorganisms, let alone the study of the motility of microorganisms

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