A microfluidic chip for detecting blood-related proteins in Alzheimer's disease associated with depression, its preparation method and application

Abstract

The invention discloses a microfluidic chip for detecting blood related proteins of Alzheimer's disease accompanied by depression and a preparation method and application thereof. The microfluidic chip aims at the deficiencies of existing detection methods, and the microfluidic chip comprises a white blood cell filter pool, a red blood cell filter pool and a platelet filter pool. The microfluidicchip can be used to directly test a blood sample without the need of blood centrifuging to obtain serum for detection. In addition, by setting an independent microfluidic detection unit, detected related proteins do not interfere with each other. At the same time, a magnetic control valve is arranged in the chip, and the synchronization and automation of the detection can be realized by controlling a switch of an electromagnetic conversion instrument. The microfluidic chip has the advantages such as simple sample processing, short detection time, low price, controllable operation, and automatic detection.

Description

technical field [0001] The invention relates to a microfluidic chip and its preparation method and application, in particular to a microfluidic chip for detecting blood-related proteins in Alzheimer's disease associated with depression, its preparation method and application. The invention belongs to the technical field of medicine. Background technique [0002] Alzheimer's disease (Alzheimer's disease) is one of the common senile, neurological, and degenerative diseases in our country. Its incidence rate is increasing year by year, and the incidence rate of women is significantly higher than that of men. Alzheimer's disease in our country is prone to complications when treated with western medicine, which is the same as Taipale et al.'s study in 2017 (Taipale H, Tolppanen AM, Koponen M, et al. Risk of pneumonia associated within incident benzodiazepine use among community dwelling adults with Alzheimerdisease [J]. CMAJ, 2017, 189(14): E519-E529.). [0003] We know that cl...

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Problems solved by technology

[0006] In fact, the specific diagnostic markers for Alzheimer's disease patients with depression are mainly concentrated in the brain tissue of the body, but the light microscope morphology and electron microscope ultramicroscopic structure of Alzheimer's disease patients with depression brain tissue The structure is relatively difficult (because the brain tissue of such patients cannot be obtained), and some large-scale nuclear magnetic resonance imaging, cerebral angiography and other technologies are expensive and the operation process is complicated, and professional technicians are required to perform the operation to display Alzheimer's disease The basic structure of the brain tissue of patients with depression. At the same time, most of the previous microfluidic chips needed to centrifuge blood to prepare serum before testing, which would increase the detection time of microfluidic chips and affect the microfluidics. In addition, the previous microfluidic chips were not able to synchronize and automate the detection of multiple blood-related proteins. These shortcomings have seriously affected the primary screening and disease evolution of Alzheimer's disease patients with depression. , drug efficacy determination, etc., for this reason, a rapid diagnosis, simple operation, low price, and strong practicability have been developed for the treatment of Alzheimer's disease patients with depression. 1-40 , Aβ 1-42 , histone H1, α2-macroglobulin, apolipoprotein 4, and brain-derived nerve growth factor detection microfluidic chip is particularly important

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