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Cancer treatment drug and application

A cancer treatment and drug technology, applied in drug combinations, antineoplastic drugs, pharmaceutical formulations, etc., can solve the problems of cisplatin resistance, toxic and side effects, etc., and achieve the effects of reducing toxic and side effects, enhancing activity, and improving therapeutic effect

Inactive Publication Date: 2018-11-06
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] An object of the present invention is to provide a combined drug regimen, that is, a combined application of ascidian polypeptide and cisplatin, in order to solve the defects of existing cisplatin resistance and toxic side effects

Method used

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  • Cancer treatment drug and application
  • Cancer treatment drug and application
  • Cancer treatment drug and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 The combined application of cisplatin and sea squirt polypeptide significantly inhibits the growth of liver cancer cells

[0032] Ascidian polypeptide is protein dry powder preparation, which can be Ciona polypeptide or Ascidian polypeptide CS5931.

[0033] Step 1: Cultivate HepG2 liver cancer cells, digest the cells with trypsin after the cells are stable, then take appropriate cells and mix them evenly, inoculate them into 96-well plates, 6000 cells per well, and place them at 37°C, 5% CO 2 in a constant temperature and humidity carbon dioxide incubator. After 24 hours, they were treated with a single drug, and the drugs used were cyclophosphamide, cisplatin, 5-FU (fluorouracil), gemcitabine, methotrexate, paclitaxel, and ascidian polypeptide; for combined drug treatment, the drugs used were fluorouracil + Ascidian polypeptide, paclitaxel + ascidian polypeptide, cyclophosphamide + ascidian polypeptide, cisplatin + ascidian polypeptide, gemcitabine + ascidia...

Embodiment 2

[0036] Example 2 Effects of ascidian polypeptide, cisplatin, and combined drugs on the growth, clone formation, and apoptosis of liver cancer cells

[0037] Step 1: After taking an appropriate amount of cells and mixing them evenly, inoculate them into a 96-well plate. The inoculation volume of HepG2 was 6000 per well, and the inoculation volume of Bel-7402 was 4000 per well. Placed at 37°C, 5% CO 2 in a constant temperature and humidity carbon dioxide incubator. After 24 hours, add to each well according to the control group (without any chemotherapeutic drugs), cisplatin group (16.67 μM), ascidian polypeptide group (14.29 μM), cisplatin (16.67 μM) + ascidian polypeptide (14.29 μM) group, Three replicate wells were made for each group of drugs, and the culture was continued under this condition. Measure the OD value of each well every 24 hours, replace the new culture medium every 48 hours, the new culture medium can be 10% serum at 1640, and draw the growth curve of each ...

Embodiment 3

[0043] Example 3 Effect of Ascidian Polypeptide and Cisplatin Combination Medication Scheme on Subcutaneous Transplanted Tumor Model of Liver Cancer

[0044] HepG2 cells were inoculated subcutaneously in the armpit of Balb / c nude nude mice. After the tumor size reached the administration conditions, the four groups of nude mice were injected with solvent (10% DMSO solution) and cisplatin + solvent (10% DMSO solution) in the abdomen respectively. , Ascidian polypeptide+solvent (10% DMSO solution) and combined drug+solvent (10% DMSO solution). The injection doses were: 5 mg / kg cisplatin plus 10% DMSO of the same volume, 50 mg / kg of ascidian polypeptide plus 10% DMSO of the same volume, 5 mg / kg of cisplatin + 50 mg / kg of ascidian polypeptide plus 10% DMSO of the same volume, and the control Group was the same volume of 10% DMSO. Doses were administered once daily, and tumor volumes were measured and analyzed. After two weeks of drug treatment, the nude mice were euthanized, the...

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Abstract

The invention discloses a cancer treatment drug which comprises sea squirt polypeptide and cis-platinum. The invention further discloses an application of a combination of the sea squirt polypeptide and the cis-platinum in preparation of the cancer treatment drug. The combination of the sea squirt polypeptide and the cis-platinum shows an obvious synergetic effect and can improve treatment efficacy of the cis-platinum on a liver cancer patient and life quality of the liver cancer patient. The combined medication scheme provides a new treatment clue and a scheme for clinical liver cancer treatment and has great development potentials and application prospects for the clinical liver cancer treatment.

Description

technical field [0001] The invention belongs to the field of biochemistry and biomedicine, in particular to a medicine for treating cancer and its application. Background technique [0002] Chinese patients with liver cancer account for 1 / 2 of the global liver cancer patients, and liver cancer is one of the top three malignant tumors in terms of proportion and death rate among cancer patients in China. Due to the large number of hepatitis B and C virus carriers in China, the number of liver cancer patients in China will further increase in the future. Cisplatin is a commonly used chemotherapeutic drug for cancer treatment, but long-term use can cause tumor drug resistance. In addition, cisplatin has side effects such as vomiting, diarrhea, and organ damage in patients. [0003] Existing treatment options for liver cancer usually use surgery, intervention, chemotherapy, and radiotherapy. Cisplatin is a commonly used drug for the treatment of liver cancer, but cisplatin has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P35/00A61P1/16A61K33/24
CPCA61K33/24A61K38/1767A61P1/16A61P35/00A61K2300/00
Inventor 林秀坤乔淦曹寿松
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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