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Processing method of cattle hide

A processing method and cowhide technology, which is applied in the field of extraction, can solve the problems of amino group molecular structure damage, material residue, product function reduction, etc., and achieve the effect of improving leather structure, increasing yield and purity, and improving purity

Pending Publication Date: 2018-11-06
广西山水牛畜牧业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantages of the acid and alkali methods are that the process is simple, the cost is low, and it can solve the fishy smell problem of collagen, but it will cause denaturation of collagen, damage the molecular structure of amino groups, and change from a tripeptide structure suitable for human absorption to a messy peptide chain. structure, the absorption rate decreases, and the product function decreases; the enzymatic process is an ideal and safe technology at present, and will not destroy the molecular structure, but the corresponding extraction cost is also high, and the above extraction methods all have material residues that destroy the extracted collagen The disadvantages of structure, low product yield, and insufficient purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for processing cowhide, comprising: (1) steaming; (2) alkali leaching; (3) acid leaching; (4) filtering; (5) enzymatic hydrolysis; (6) solid-liquid separation; Enzymolysis treatment was carried out at a microwave temperature of 20°C for 60 minutes. The enzymolysis preparation included the following raw materials: 2 kg of cellulase, 1 kg of bacterial marine enzyme, 1 kg of banana peel protease, 3 kg of trypsin, 1 kg of pectinase, and 3 kg of grapefruit peel protease ; The dosage of the enzymolysis preparation is 0.8% of the cowhide quality;

[0026] The steaming is to heat-treat the cowhide at a steam temperature of 120°C until it is half-cooked;

[0027] The alkali immersion is to soak the cowhide in an alkaline solution at a temperature of 80°C for 15 minutes;

[0028] The alkaline solution is composed of the following substances in mass fraction: 5% of theophylline, 0.7% of matrine, 0.% of ephedrine, and the balance is food-grade ethanol solution;

[0029] ...

Embodiment 2

[0037] A method for processing cowhide, comprising: (1) steaming; (2) alkali leaching; (3) acid leaching; (4) filtering; (5) enzymatic hydrolysis; (6) solid-liquid separation; Under the condition of microwave temperature of 25°C, the enzymolysis treatment was carried out for 90 minutes. The enzymolysis preparation included the following raw materials: 6 kg of cellulase, 3 kg of bacterial marine enzyme, 4 kg of banana peel protease, 7 kg of trypsin, 7 kg of pectinase, and 5 kg of grapefruit peel protease ; The consumption of the enzymolysis preparation is 4% of cowhide quality;

[0038] The steaming is to heat-treat the cowhide at a steam temperature of 128°C until it is half-cooked;

[0039] The alkali immersion is to soak the cowhide in an alkaline solution at a temperature of 95°C for 20 minutes;

[0040] The alkaline solution is composed of the following substances in mass fraction: 13% of theophylline, 1.1% of matrine, 0.7% of ephedrine, and the balance is food-grade etha...

Embodiment 3

[0049] A method for processing cowhide, comprising: (1) steaming; (2) alkali leaching; (3) acid leaching; (4) filtering; (5) enzymatic hydrolysis; (6) solid-liquid separation; The enzymolysis treatment was carried out at a microwave temperature of 25°C for 90 minutes. The enzymolysis preparation included the following raw materials: 4 kg of cellulase, 2 kg of bacterial marine enzyme, 2.5 kg of banana peel protease, 5 kg of trypsin, 4 kg of pectinase, and 4 kg of grapefruit peel protease. 4kg; the consumption of the enzymolysis preparation is 2.5% of cowhide quality;

[0050] The steaming is to heat-treat the cowhide at a steam temperature of 125°C until it is half-cooked;

[0051] The alkali immersion is to soak the cowhide in an alkaline solution at a temperature of 90°C for 18 minutes;

[0052] The alkaline solution is composed of the following substances in mass fraction: 7% of theophylline, 1% of matrine, 0.5% of ephedrine, and the balance is food-grade ethanol solution; ...

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Abstract

The invention relates to the technical field of extraction and especially relates to a processing method of cattle hide. The processing method comprises the following steps: (1) steaming, (2) alkali dipping, (3) acid dipping, (4) filtering, (5) enzymolysis and (6) solid-liquid separation, wherein enzymolysis treatment is performed at a microwave temperature of 20-25 DEG C for 60-90min; an enzymolysis preparation contains the following raw materials in parts by weight: 2-6 parts of cellulase, 1-3 parts of marine bacterial enzymes, 1-4 parts of banana peel protease, 3-7 parts of trypsin, 1-7 parts of pectinase and 3-5 parts of pomelo peel protease; and the usage of the enzymolysis preparation is 0.8-4% of the mass of the cattle hide. The influence of impurities is reduced and collagen is extracted to the greatest extent by the method disclosed by the invention, thereby being helpful to further improve the yield of collagen.

Description

technical field [0001] The invention relates to the technical field of extraction, in particular to a method for processing cowhide. Background technique [0002] Collagen is the main component of the extracellular matrix, which widely exists in the connective tissue of animals, accounting for 25% to 30% of the total protein in the animal body. The structural feature of collagen is a triple helix structure, the molecular structure is very stable, and it has low immunogenicity and good biodegradability. As a natural biological resource, collagen has unique biocompatibility and biodegradability , as a biological material, widely used in medicine, food, cosmetics and other fields. [0003] In the prior art, methods for extracting collagen include processes such as acid, alkali, and enzymatic methods. The advantages of acid and alkali methods are that the process is simple, the cost is low, and it can solve the fishy smell problem of collagen, but it will cause denaturation of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/10A23L13/20A23L29/00
CPCA23L5/10A23L5/13A23L13/20A23L29/06
Inventor 谭春萍杨远澄周贵金陆海龙杨远广
Owner 广西山水牛畜牧业有限责任公司
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